| AS8351histone demethylase inhibitor |
Sample solution is provided at 25 µL, 10mM.
Nature.2017 Jan 19;541(7637):417-420.
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- Purity = 98.00%
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- NMR (Nuclear Magnetic Resonance)
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Chemical structure
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| Cas No. | 796-42-9 | SDF | Download SDF |
| Chemical Name | (E)-N"-((2-hydroxynaphthalen-1-yl)methylene)isonicotinohydrazide | ||
| Canonical SMILES | OC(C=CC1=CC=CC=C12)=C2/C([H])=N/NC(C3=CC=NC=C3)=O | ||
| Formula | C17H13N3O2 | M.Wt | 291.3 |
| Solubility | ≥29.1mg/mL in DMSO | Storage | Store at -20°C |
| Physical Appearance | A solid | Shipping Condition | Evaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
IC50: N/A
AS8351 is a histone demethylase inhibitor.
Histone methylation is considered as a more dynamic modification with the discovery of Histone demethylases. Removal of methyl groups is mediated by LSD1, PAD14 and JmjC domain containing proteins.
In vitro: In a previous study, in order to identify indispensable factors for human fibroblasts, the authors removed combined compounds one by one and treated cells with the remaining compounds. Results showed that the number of beating clusters was significantly reduced by removal of seven compounds including AS8351. Moreover, these seven compounds inlcuding AS8351 were sufficient and necessary to induce cardiac reprogramming efficiently. The authors then investigated the role of the reprogramming compound of AS8351 and the results showed that AS8351 and its functional analogs could affect epigenetic modifications by competing with α- ketoglutarate for chelating iron/Fe(II) in some epigenetic enzymes, including the JmjC-domain-containing histone demethylasesthat require α-KG and iron as co-factors. In addition, the authors abrogated each of the 22 genes in the JmjC-KDM family by small hairpin RNAs and found that only with KDM5B knockdown, or using a KDM5B inhibitor, PBIT could phenocopy AS8351 in generating ciCMs, indicating that it might be a target of AS8351 [1].
In vivo: Currently, there is no animal in vivo data reported.
Clinical trial: Up to now, AS8351 is still in the preclinical development stage.
Reference:[1] N. Cao, Y. Huang, J. Zheng,et al.Conversion of human fibroblasts into functional cardiomyocytes by small molecules.Science 352(6290),1216-1220(2016).
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师兄说每次一定要两块胶一起跑电泳才能形成回路，但是制胶因为只有一个制胶架，就只能分两次弄
想问一下一般都是这样的吗？还有加了浓缩胶插了梳子之后一定要等2h才能让胶充分凝固吗？
小弟这个学期开学以来，电泳槽的液体因为假期无人看管就干了，我用了酒精冲洗好几遍后，电泳槽泡出来的电泳就一直有问题，当你第一次用新电泳液跑的时候还比较好，但是用了两三次之后就会摸带，需要极其频繁的更换电泳液，纯水消耗量极大，请问各位大神如何修复？
TIF格式的图片无法上传。。。。如果需要图片我再转格式发过来，谢谢各位。
