- Description
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Description
Details
Description:Rabbit anti-Serine 468 phosphorylated NF-κB p65 polyclonal antibody
Purification: Protein G affinity purified
Product Type:Primary antibody
Target Protein:Serine 468 phosphorylated NF-κB p65
Immunogen:A synthetic peptide (FTDLASpVDNSE) derived from Serine 468 phosphorylated NF-κB p65, conjugated with KLH for immunization
Specificity:This antibody is reactive to the synthetic NF-κB-p65 peptide (FTDLASpVDNSE).
Species Reacitvity:Mouse, human and other species with consensus serine 468 phosphorylated NF-κB-p65 peptide (FTDLASpVDNSE)
Source:Rabbit
Formulation:Lyophilized in pH 7.4, 0.01M PBS
Reconstitution:Double distilled water is recommended to adjust the final concentration to 1.00mg/mL.
Storage:Store at -20oC
Research Area:Life science research, stem cell factor signalling, cancer research
Background:
NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) is a transcription factor that controls the transcription of many genes that are associated with immuno-response, anti-apoptosis, neuroplasticity and memory. There are five NF-κB proteins in mammals: RelA (NF-κB-p65), RelB, c-Rel, NF-kB (NF-κB-p105), and NF-κB2 (NF-κB-p100). They form a variety of homodimers and heterodimers, each of which activates its own distinctive set of genes. P65 has been shown to be involved with DNA binding. The phosphorylation of p65 at Ser468 is inducible by TNF. The phosphorylation mediates p65 ubiquitination and the termination of related gene expression.
Application:
ELISA:The antibody (approx. 1:50,000 -1:120,000 diluted) can detect the serine 468 phosphorylated NF-κB p65 peptide coated on ELISA plate at 100ng/well.
Other applications have not been evaluated.
References:
If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.
Additional
Additional Information
| Product Specificity | pAb Rabbit anti-Phospho-NF-қB-p65 (Ser468) |
|---|---|
| Application | EIA |
| Size | 0.1 mg |
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小弟这个学期开学以来，电泳槽的液体因为假期无人看管就干了，我用了酒精冲洗好几遍后，电泳槽泡出来的电泳就一直有问题，当你第一次用新电泳液跑的时候还比较好，但是用了两三次之后就会摸带，需要极其频繁的更换电泳液，纯水消耗量极大，请问各位大神如何修复？
TIF格式的图片无法上传。。。。如果需要图片我再转格式发过来，谢谢各位。
师兄说每次一定要两块胶一起跑电泳才能形成回路，但是制胶因为只有一个制胶架，就只能分两次弄
想问一下一般都是这样的吗？还有加了浓缩胶插了梳子之后一定要等2h才能让胶充分凝固吗？
