Human SUMO2/3 Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.
Data Examples
View LargerDetection of Human SUMO2/3 by Western Blot.Western blot shows lysates of SUMO2/3. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human SUMO2/3 Monoclonal Antibody (Catalog # A-718) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for SUMO2/3 at approximately 16-20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
View LargerDetection of Human SUMO2/3 by Western Blot.Western blot shows lysates of Jurkat human acute T cell leukemia cell line and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human SUMO2/3 Monoclonal Antibody (Catalog # A-718) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for SUMO2/3 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
Background: SUMO2/3
Small Ubiquitinlike Modifiers (SUMOs) are a family of small, related proteins that can be enzymatically attached to a target protein by a posttranslational modification process termed SUMOylation. Unlike ubiquitination, which targets proteins for degradation, SUMOylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. All human SUMO proteins share a conserved ubiquitin-like domain and a C-terminal diglycine cleavage/attachment site. Human SUMO1, SUMO2, SUMO3, and SUMO4 are all translated as propeptides, containing C-terminal prosegments following the diglycine motif that marks the end of the mature forms. Following prosegment cleavage, SUMO1, 2, and 3 may then be enzymatically attached to a lysine on a target protein. It is not clear whether SUMO4 is processed in a similar fashion.
Product Datasheets
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsIsotype Controls
Normal Rat IgG Control(Azide Free)
Rat IgG2A Isotype Control
Rat IgG2A Isotype Control
Normal Rat IgG Control
Secondary Antibodies
Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody
Rat F(ab)2 IgG (H+L) PE-conjugated Antibody
Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody
Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody
Goat Anti-Rat F(ab)2 IgG (H+L) APC-conjugated Antibody
Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody
Goat Anti-Rat IgG Biotinylated Antibody
Rat IgG HRP-conjugated Antibody
Rat IgG VisUCyte HRP Polymer Antibody
Goat Anti-Rat IgG Antibody
Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody
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师兄说每次一定要两块胶一起跑电泳才能形成回路，但是制胶因为只有一个制胶架，就只能分两次弄
想问一下一般都是这样的吗？还有加了浓缩胶插了梳子之后一定要等2h才能让胶充分凝固吗？
