| Application | Each vial is sufficient for a volume of 100ml solution |
|---|---|
| Just add to your cell lysis buffer |
The Xpert Phosphatase Inhibitor Cocktail Solution(100X)
is a mixture of several inhibitors to prevent valuable
proteinsfrom dephospholylation. Phosphorylation and
dephosphorylation is a molecular on/off switch that regulates
a number of key biological pathways within the cell,
including signal transduction, cell division, and apoptosis.
The Xpert Phosphatase Inhibitor Cocktail preserves
phosphorylation of proteins in cell lysates and tissue
extracts.The cocktail contains a mixture of four
phosphatase inhibitors of broad specificity, including
sodium fluoride, sodium orthovanadate, sodium
pyrophosphateand sodium glycerophosphate.
Unlike other commerciallyavailable phosphatase
inhibitor cocktails which protectagainst either
Ser/Thr phosphatases or protein tyrosine
phosphatases.
The Xpert Phosphatase Inhibitor Cocktail protects
phosphoproteins from both families of phosphatases.
Conveniently packaged in a 100X format, the cocktail
is stable at 4°C and is compatible with common protein
assays, including the BCA Protein Assay and Bradford-
basedassays such as Coomassie.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
配制分三步: 1) 1 M Tris-HCl (pH 8.0) 50 ml的配制:称取Tris碱6.06 g,加超纯水40 ml溶解,滴加浓HCl约2.1 ml调pH至8.0,定容至50 ml。
电泳缓冲液的另一个作用是使溶液具有一定的导电性,以利于DNA分子的迁移,例如,一般电泳缓冲液中应含有0.01-0.04mol/L的Na+离子,Na+离子的浓度太低时电泳速度变慢;太高时就会造成过大的电流使胶发热甚至熔化。
电泳缓冲液还有一个组分是EDTA,加入浓度为1-2mmol/L,目的是螯合Mg2+ 等离子,防止电泳时激活 DNA酶,此外还可防止Mg2+离子与核酸生成沉淀。

