
5A-ANDROSTAN-3,17-DIONE(ANDROSTANEDIONE) (2,3,4-13C3, 98%)
This is a controlled substance. All orders forthis item must be accompanied by a copy of thecustomer"s valid DEA permit (electronic copy isok). For International and Canadian orders: an enduse Statement will be required in addition to theDEA permit. If the item is not controlled bycountry of ultimate destination, CIL will need aLetter of no Objection in place of the DEA permit.
Item Number | CLM-10548-PK |
Chemical Formula | |
Unlabeled CAS# | 846-46-8 |
Labeled CAS# | |
Molecular Weight* | |
Chemical Purity | 98% |
* For isotopically labeled compounds, MW listed is for the fully enriched product. |
ebiomall.com






>
>
>
>
>
>
>
>
>
>
>
>
因为DNA和RNA链上的碱基都基本一致。而这两种上样缓冲液中含有的染料是二甲苯青和溴酚蓝,都可以与其碱基结合,跑出条带。
但是,如果使用DNA上样缓冲液,里面会夹杂一些RNA酶,这些酶有可能会影响到实验结果。使用前需要将RNA酶处理掉。
1mol/l Tirs-HCl(PH8.8) 0.6ml
10%SDS 2ml
50%甘油 5ml
2-巯基乙醇 0.5ml
1%溴酚兰 1ml
水 0.9ml
另外:SDS不好溶解建议加热溶解,如果要做非还原SDS-PAGE请将2-巯基乙醇 0.5ml换成超纯水即可
配制分三步: 1) 1 M Tris-HCl (pH 8.0) 50 ml的配制:称取Tris碱6.06 g,加超纯水40 ml溶解,滴加浓HCl约2.1 ml调pH至8.0,定容至50 ml。

