5A-ANDROSTAN-3,17-DIONE(ANDROSTANEDIONE) (2,3,4-13C3, 98%)
This is a controlled substance. All orders forthis item must be accompanied by a copy of thecustomer"s valid DEA permit (electronic copy isok). For International and Canadian orders: an enduse Statement will be required in addition to theDEA permit. If the item is not controlled bycountry of ultimate destination, CIL will need aLetter of no Objection in place of the DEA permit.
Item Number | CLM-10548-PK |
Chemical Formula | |
Unlabeled CAS# | 846-46-8 |
Labeled CAS# | |
Molecular Weight* | |
Chemical Purity | 98% |
* For isotopically labeled compounds, MW listed is for the fully enriched product. | |
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加样缓冲液的主要作用是使PCR产物与其混合,
使DNA沉于加样孔的底部,防止DNA跑出来.
1mol/l Tirs-HCl(PH8.8) 0.6ml
10%SDS 2ml
50%甘油 5ml
2-巯基乙醇 0.5ml
1%溴酚兰 1ml
水 0.9ml
另外:SDS不好溶解建议加热溶解,如果要做非还原SDS-PAGE请将2-巯基乙醇 0.5ml换成超纯水即可
电泳缓冲液的另一个作用是使溶液具有一定的导电性,以利于DNA分子的迁移,例如,一般电泳缓冲液中应含有0.01-0.04mol/L的Na+离子,Na+离子的浓度太低时电泳速度变慢;太高时就会造成过大的电流使胶发热甚至熔化。
电泳缓冲液还有一个组分是EDTA,加入浓度为1-2mmol/L,目的是螯合Mg2+ 等离子,防止电泳时激活 DNA酶,此外还可防止Mg2+离子与核酸生成沉淀。

