TriForest Erlenmeyer Shaker Flasks are manufactured with virgin, optically clear, non leaching LEXAN® Polycarbonate resin that retains clarity after gamma radiation. The flasks are certified pyrogen free, DNase/RNase free, ideal for cell cultures, microbial cultures, plants, media preparation, storage, and all related applications. They fit all standard shaking incubator clamps and can be placed on any platform. Proudly Manufactured in the USA!
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配制分三步: 1) 1 M Tris-HCl (pH 8.0) 50 ml的配制:称取Tris碱6.06 g,加超纯水40 ml溶解,滴加浓HCl约2.1 ml调pH至8.0,定容至50 ml。
电泳缓冲液的另一个作用是使溶液具有一定的导电性,以利于DNA分子的迁移,例如,一般电泳缓冲液中应含有0.01-0.04mol/L的Na+离子,Na+离子的浓度太低时电泳速度变慢;太高时就会造成过大的电流使胶发热甚至熔化。
电泳缓冲液还有一个组分是EDTA,加入浓度为1-2mmol/L,目的是螯合Mg2+ 等离子,防止电泳时激活 DNA酶,此外还可防止Mg2+离子与核酸生成沉淀。

