
Sonibeast tube with Locking Lid - 0.6 ml
Cat. No. L-502-GRD-Q, 0.6 ml polypropylene tube with locking cap, 1000 count (for SoniBeast disrupter)
Our Price : $0.00
The PCR tubes used with the SoniBeast™ Cell Disrupter require locking lids. Due to very high mixing energies of the SoniBeast, common, non-locking PCR tubes may leak during used in the SoniBeast. The Sonibeast PCR tube with Locking Lid has a small ball flexibly tethered to the vial body which engages a groove in the lid, thus providing positive confirmation that the tube is securely locked and remains tightly closed during beadbeating.
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加样缓冲液的主要作用是使PCR产物与其混合,
使DNA沉于加样孔的底部,防止DNA跑出来.
1mol/l Tirs-HCl(PH8.8) 0.6ml
10%SDS 2ml
50%甘油 5ml
2-巯基乙醇 0.5ml
1%溴酚兰 1ml
水 0.9ml
另外:SDS不好溶解建议加热溶解,如果要做非还原SDS-PAGE请将2-巯基乙醇 0.5ml换成超纯水即可
电泳缓冲液的另一个作用是使溶液具有一定的导电性,以利于DNA分子的迁移,例如,一般电泳缓冲液中应含有0.01-0.04mol/L的Na+离子,Na+离子的浓度太低时电泳速度变慢;太高时就会造成过大的电流使胶发热甚至熔化。
电泳缓冲液还有一个组分是EDTA,加入浓度为1-2mmol/L,目的是螯合Mg2+ 等离子,防止电泳时激活 DNA酶,此外还可防止Mg2+离子与核酸生成沉淀。

