
Sonibeast tube with Locking Lid - 0.6 ml
Cat. No. L-502-GRD-Q, 0.6 ml polypropylene tube with locking cap, 1000 count (for SoniBeast disrupter)
Our Price : $0.00
The PCR tubes used with the SoniBeast™ Cell Disrupter require locking lids. Due to very high mixing energies of the SoniBeast, common, non-locking PCR tubes may leak during used in the SoniBeast. The Sonibeast PCR tube with Locking Lid has a small ball flexibly tethered to the vial body which engages a groove in the lid, thus providing positive confirmation that the tube is securely locked and remains tightly closed during beadbeating.
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配制分三步: 1) 1 M Tris-HCl (pH 8.0) 50 ml的配制:称取Tris碱6.06 g,加超纯水40 ml溶解,滴加浓HCl约2.1 ml调pH至8.0,定容至50 ml。
电泳缓冲液的另一个作用是使溶液具有一定的导电性,以利于DNA分子的迁移,例如,一般电泳缓冲液中应含有0.01-0.04mol/L的Na+离子,Na+离子的浓度太低时电泳速度变慢;太高时就会造成过大的电流使胶发热甚至熔化。
电泳缓冲液还有一个组分是EDTA,加入浓度为1-2mmol/L,目的是螯合Mg2+ 等离子,防止电泳时激活 DNA酶,此外还可防止Mg2+离子与核酸生成沉淀。

