| Product Name | β - Casomorphin (1 - 7), bovineYPFPGPI |
| Size | 1 mg |
| Catalog # | AS-61124 |
| US$ | $73 |
| Purity | % Peak Area By HPLC ≥ 95% |
β-Casomorphin-7 is a milk opioid peptide derived from fragment 60-66 of bovine beta-Casein. It strongly stimulates mucin secretion in the rat jejunum through a nervous pathway and mu-opioid receptor activation. The presence of the Tyr-Pro aromatic sequence makes the peptide selective for the mu receptor while the high contents of proline residues in the sequence makes the peptide resistant to degradation by gastrointestinal enzymes. | |
| Detailed Information | |
| Storage | -20°C |
| References | Brantl, V. et al. Hoppe Seylers Z Physiol Chem 360, 1211 (1979) Zoghbi, S. et al. Am J Physiol - Gastrointestinal Liver Physiol 290, G1105, doi: 10.1152/ajpgi.00455.2005 Trompette, A. et al. Am J Nutrition 133, 3499 (2003) |
| Molecular Weight | 789.9 |
| YPFPGPI | |
| Sequence(Three-Letter Code) | H - Tyr - Pro - Phe - Pro - Gly - Pro - Ile - OH |
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配制分三步: 1) 1 M Tris-HCl (pH 8.0) 50 ml的配制:称取Tris碱6.06 g,加超纯水40 ml溶解,滴加浓HCl约2.1 ml调pH至8.0,定容至50 ml。
电泳缓冲液的另一个作用是使溶液具有一定的导电性,以利于DNA分子的迁移,例如,一般电泳缓冲液中应含有0.01-0.04mol/L的Na+离子,Na+离子的浓度太低时电泳速度变慢;太高时就会造成过大的电流使胶发热甚至熔化。
电泳缓冲液还有一个组分是EDTA,加入浓度为1-2mmol/L,目的是螯合Mg2+ 等离子,防止电泳时激活 DNA酶,此外还可防止Mg2+离子与核酸生成沉淀。

