
Product Name | β - Casomorphin (1 - 7), bovineYPFPGPI |
Size | 1 mg |
Catalog # | AS-61124 |
US$ | $73 |
Purity | % Peak Area By HPLC ≥ 95% |
β-Casomorphin-7 is a milk opioid peptide derived from fragment 60-66 of bovine beta-Casein. It strongly stimulates mucin secretion in the rat jejunum through a nervous pathway and mu-opioid receptor activation. The presence of the Tyr-Pro aromatic sequence makes the peptide selective for the mu receptor while the high contents of proline residues in the sequence makes the peptide resistant to degradation by gastrointestinal enzymes. | |
Detailed Information | ![]() |
Storage | -20°C |
References | Brantl, V. et al. Hoppe Seylers Z Physiol Chem 360, 1211 (1979) Zoghbi, S. et al. Am J Physiol - Gastrointestinal Liver Physiol 290, G1105, doi: 10.1152/ajpgi.00455.2005 Trompette, A. et al. Am J Nutrition 133, 3499 (2003) |
Molecular Weight | 789.9 |
YPFPGPI | |
Sequence(Three-Letter Code) | H - Tyr - Pro - Phe - Pro - Gly - Pro - Ile - OH |
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最好是测浓度,一般不超过30-40ug,上样缓冲液一般4×或者5×,这个没太大区别。
蛋白电泳上样缓冲液包括2%SDS , 0.1%溴酚蓝 10%甘油,SDS是保证样品中的所有蛋白带电荷一致,减少电荷对电泳结果的影响。还原剂是让二硫键处于断开状态,保证蛋白分子的线性.
溴酚蓝作用是指示上样蛋白的跑胶时标记的,甘油是增加上样重量的,以免漂浮,煮沸是使蛋白变性的永久保存
加样缓冲液的主要作用是使PCR产物与其混合,
使DNA沉于加样孔的底部,防止DNA跑出来.
2乘SDS-PAGE上样缓冲液(20ml体系)
1.0mol/L Tris-HCL (pH 6.8) 2ml
1.0mol/L DTT 4ml
SDS 0.8g
溴芬蓝 0.04g
甘油 4ml
dd水 定容至20ml
4℃冰箱保存,可以不分装,我们实验室一般用三个月以上
先配1.0mol/L的DTT,双蒸无菌水溶解4℃冰箱保存,配上样缓冲液的时候加入就可以了,都混匀保存也没问题的

