| Product Name | HiLyteᵀᴹFluor 647 acid, SEHiLyte Fluor 647 acid, NHS ester |
| Size | 1 mg |
| Catalog # | AS-81256 |
| US$ | $249 |
| Purity | ≥95% by HPLC |
HiLyte Fluor 647 acid, SE is an excellent fluorescent labeling dye that generates the protein conjugates that are slightly red-shifted compared to those of Cy5 dyes, resulting in an optimal match to filters designed for Cy5 dyes. However, total fluorescence of the secondary antibody conjugates of HiLyte Fluor 647 dyes is significantly higher than that of Cy5 conjugates supplied by other companies. Also, unlike Cy5 dyes, HiLyte Fluor 647 dyes have very little change in absorption or fluorescence spectra when conjugated to most proteins, oligonucleotides and nucleic acids, thus yielding greater total fluorescence at the same degree of substitution. Additionally, our in-house data indicated that HiLyte Fluor 647 is superior to Cy5 in fluorescence polarization-based assays.Extinction Coefficient (M-1cm-1): 250,000Fluorescence quantum yield: 0.33Fluorescence Life Time (ns): 1.0
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| Detailed Information | |
| Storage | -20°C desiccated and protected from light |
| Molecular Weight | 1302.71 |
| Spectral Properties | Abs/Em = 649/674 nm |
| Solvent System | DMF or DMSO |
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配制分三步: 1) 1 M Tris-HCl (pH 8.0) 50 ml的配制:称取Tris碱6.06 g,加超纯水40 ml溶解,滴加浓HCl约2.1 ml调pH至8.0,定容至50 ml。
电泳缓冲液的另一个作用是使溶液具有一定的导电性,以利于DNA分子的迁移,例如,一般电泳缓冲液中应含有0.01-0.04mol/L的Na+离子,Na+离子的浓度太低时电泳速度变慢;太高时就会造成过大的电流使胶发热甚至熔化。
电泳缓冲液还有一个组分是EDTA,加入浓度为1-2mmol/L,目的是螯合Mg2+ 等离子,防止电泳时激活 DNA酶,此外还可防止Mg2+离子与核酸生成沉淀。


