![StressMarq/Anti-DUX4 Antibody [P2B1]/SMC-192D-ALP/100-µg](images/StressMarq/201710/SMC-192_DUX4_Antibody_P2B1_ICC-IF_Mouse_C2C12-myoblast-cells_1-150x150.png)
Overview:
Product Name | DUX4 Antibody | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Description | Mouse Anti-Human DUX4 Monoclonal IgG1 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Species Reactivity | Human, Mouse | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Applications | WB, IHC, ICC/IF | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Antibody Dilution | WB (1:1000), ICC/IF (1:1000); optimal dilutions for assays should be determined by the user. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Host Species | Mouse | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Immunogen Species | Human | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Immunogen | C-terminal 76 amino acids of DUX4 with glutathione-s-transferase (gst) tag | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Concentration | 1 mg/ml | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Conjugates |
Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
StreptavidinProperties:
Streptavidin Datasheet BiotinProperties:
Biotin Datasheet HRP (Horseradish peroxidase)Properties:
HRP Datasheet AP (Alkaline Phosphatase)Properties:
AP Datasheet
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Properties
Storage Buffer | PBS pH7.4, 50% glycerol, 0.09% sodium azide |
Storage Temperature | -20ºC |
Shipping Temperature | Blue Ice or 4ºC |
Purification | Protein G Purified |
Clonality | Monoclonal |
Clone Number | P2B1 |
Isotype | IgG1 |
Specificity | Detects ~45kDa. No cross-reactivity with DUX4c. |
Cite This Product | StressMarq Biosciences Cat# SMC-192, RRID: AB_10964369 |
Certificate of Analysis | 1 µg/ml of SMC-192 was sufficient for detection of DUX4 in 20 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-mouse IgG: HRP as teh secondary. |
Biological Description
Alternative Names | Double homeobox 4 Antibody, Double homeobox 10 Antibody, DUX10 Antibody, Double homeobox protein 4/10 Antibody |
Research Areas | Cell Signaling |
Cellular Localization | Nucleus |
Accession Number | NP_149418.3 |
Gene ID | 22947 |
Swiss Prot | F5GZ66 |
Scientific Background | DUX4, or double homeobox4, is a human protein that is a transcriptional activator of paired-like homeodomain transcription factor 1 (1). Clinically it is a facioscapulohumeral muscular dystrophy candidate gene that appears to have a toxic gain of function (2-4). In FSHD individuals, the expression of the full-length DUX4 transcript is not completely suppressed in skeletal muscle and possibly other differentiated tissues (5). |
References |
1. Entrez Gene: “Dux4 Double Homeobox, 4” 2. Dixit M., et al., (2007) Proc. Natl Acad Sci. USA. 104(46): 18157-18162. 3. Kowaljow V., et al. (2007) Neuromuscl Disord. 17(8): 611-623. 4. Lemmers R., et al. (2010) Science Express. 329(5999): 1650-1653. 5. Snider L., et al. (2010) PLoS Genetics. 6:1-14. |
Product Images

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-DUX4 Monoclonal Antibody, Clone P2B1 (SMC-192). Tissue: C2C12 myoblast cells. Species: Mouse. Primary Antibody: Mouse Anti-DUX4 Monoclonal Antibody (SMC-192) at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: DAPI (blue) nuclear stain.

Western Blot analysis of Mouse C2C12 cell lysate showing detection of DUX4 protein using Mouse Anti-DUX4 Monoclonal Antibody, Clone P2B1 (SMC-192). Primary Antibody: Mouse Anti-DUX4 Monoclonal Antibody (SMC-192) at 1:1000. Cells transfected with pCS2+DUX4 which, contains an additional upstream start site.
Product Citations (0)
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AP (Alkaline Phosphatase)
Properties:
- Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
- Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
- Catalyzes the conversion of:
- Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
- Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
- Molecular weight: 140 kDa
- Applications: Western blot, immunohistochemistry, and ELISA
AP Datasheet
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① 上样缓冲液中包括甘油,溴酚蓝,SDS等,甘油主要作用是防止样品漂浮出点样孔,溴酚蓝作为电泳指示剂,用来观察电泳进度,SDS用于一些核酸结合蛋白的变性解离.
②核酸染料与凝胶中核酸结合,以便于在紫外光下显示核酸条带.
蛋白电泳上样缓冲液包括2%SDS , 0.1%溴酚蓝 10%甘油,SDS是保证样品中的所有蛋白带电荷一致,减少电荷对电泳结果的影响。还原剂是让二硫键处于断开状态,保证蛋白分子的线性.
溴酚蓝作用是指示上样蛋白的跑胶时标记的,甘油是增加上样重量的,以免漂浮,煮沸是使蛋白变性的永久保存
2乘SDS-PAGE上样缓冲液(20ml体系)
1.0mol/L Tris-HCL (pH 6.8) 2ml
1.0mol/L DTT 4ml
SDS 0.8g
溴芬蓝 0.04g
甘油 4ml
dd水 定容至20ml
4℃冰箱保存,可以不分装,我们实验室一般用三个月以上
先配1.0mol/L的DTT,双蒸无菌水溶解4℃冰箱保存,配上样缓冲液的时候加入就可以了,都混匀保存也没问题的
1mol/l Tirs-HCl(PH8.8) 0.6ml
10%SDS 2ml
50%甘油 5ml
2-巯基乙醇 0.5ml
1%溴酚兰 1ml
水 0.9ml
另外:SDS不好溶解建议加热溶解,如果要做非还原SDS-PAGE请将2-巯基乙醇 0.5ml换成超纯水即可
电泳缓冲液的另一个作用是使溶液具有一定的导电性,以利于DNA分子的迁移,例如,一般电泳缓冲液中应含有0.01-0.04mol/L的Na+离子,Na+离子的浓度太低时电泳速度变慢;太高时就会造成过大的电流使胶发热甚至熔化。
电泳缓冲液还有一个组分是EDTA,加入浓度为1-2mmol/L,目的是螯合Mg2+ 等离子,防止电泳时激活 DNA酶,此外还可防止Mg2+离子与核酸生成沉淀。
因为DNA和RNA链上的碱基都基本一致。而这两种上样缓冲液中含有的染料是二甲苯青和溴酚蓝,都可以与其碱基结合,跑出条带。
但是,如果使用DNA上样缓冲液,里面会夹杂一些RNA酶,这些酶有可能会影响到实验结果。使用前需要将RNA酶处理掉。

