StressMarq/Anti-EAAC1 Antibody [S180-41]/SMC-406D-A390/100-µg_蚂蚁淘，【正品极速】生物医学科研用品轻松购|ebiomall 蚂蚁淘商城

商品信息

联系客服

StressMarq/Anti-EAAC1 Antibody [S180-41]/SMC-406D-A390/100-µg

郑重提醒：

无质量问题不接受退换货，下单前请仔细核对信息。

下单后请及时联系客服核对商品价格，订单生效后再付款。

StressMarq/Anti-EAAC1 Antibody [S180-41]/SMC-406D-A390/100-µg

品牌 /

StressMarq

货号 /

SMC-406D-A390

官网地址

美元价：

登录查看

(友情提示：该价格仅为参考，欢迎联系客服询价！)

数量：

免费咨询热线

4000-520-616

商品介绍售后保障相关文章商品咨询

联系客服

Overview:

Product Name EAAC1 Antibody

Description

Mouse Anti-Human EAAC1 Monoclonal IgG1

Species Reactivity Human, Rat

Applications WB, IP, ICC/IF

Antibody Dilution WB (1:1000), ICC/IF (1:100);optimal dilutions for assays should be determined by the user.

Host Species Mouse

Immunogen Species Human

Immunogen Fusion protein amino acids 1-524 (full length) of human EAAC1

Concentration 1 mg/ml

Conjugates

Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

PerCP

Overview:

Peridinin-Chlorophyll-Protein Complex
Small phycobiliprotein
Isolated from red algae
Large stokes shift (195 nm)
Molecular Weight: 35 kDa

PerCP Datasheet

PerCP Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 482 nm

λ_em = 677 nm

ε_max = 1.96 x 10⁶

Laser = 488 nm

PE/ATTO 594

PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm.

Overview:

High fluorescence yield
High photostability
Very hydrophilic
Excellent solubility in water
Very little aggregation

PE/ATTO 594 Datasheet

PE-ATTO 594 Fluorophore Conjugate Excitation and Emission Spectra

Optical Properties:

λ_ex = 535 nm

λ_em = 627 nm

Laser = 488 to 561 nm

FITC (Fluorescein)

Overview:

Excellent fluorescence quantum yield
High rate of photobleaching
Good solubility in water
Broad emission spectrum
pH dependent spectra
Molecular formula: C₂₀H₁₂O₅
Molar mass: 332.3 g/mol

FITC-Fluorescent-conjugate

FITC Fluorescein Fluorophore Excitation and Emission Spectra

Optical Properties:

λ_ex = 494 nm

λ_em = 520 nm

ε_max = 7.3×10⁴

Φ_f = 0.92

τ_fl = 5.0 ns

Brightness = 67.2

Laser = 488 nm

Filter set = FITC

ATTO 700

Overview:

High fluorescence yield
Excellent thermal and photostability
Quenched by electron donors
Very hydrophilic
Good solubility in polar solvents
Zwitterionic dye
Molar Mass: 575 g/mol

ATTO 700 Datasheet

ATTO 700 Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 700 nm

λ_em = 719 nm

ε_max = 1.25×10⁵

Φ_f = 0.25

τ_fl = 1.6 ns

Brightness = 31.3

Laser = 676 nm

Filter set = Cy®5.5

ATTO 680

Overview:

High fluorescence yield
Excellent thermal and photostability
Quenched by electron donors
Very hydrophilic
Good solubility in polar solvents
Zwitterionic dye
Molar Mass: 631 g/mol

ATTO 680 Datasheet

ATTO 680 Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 680 nm

λ_em = 700 nm

ε_max = 1.25×10⁵

Φ_f = 0.30

τ_fl = 1.7 ns

Brightness = 37.5

Laser = 633 – 676 nm

Filter set = Cy®5.5

ATTO 655

Overview:

High fluorescence yield
High thermal and photostability
Excellent ozone resistance
Quenched by electron donors
Very hydrophilic
Good solubility in polar solvents
Zwitterionic dye
Molar Mass: 634 g/mol

ATTO 655 Datasheet

ATTO 655 Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 663 nm

λ_em = 684 nm

ε_max = 1.25×10⁵

Φ_f = 0.30

τ_fl = 1.8 ns

Brightness = 37.5

Laser = 633 – 647 nm

Filter set = Cy®5

ATTO 633

Overview:

High fluorescence yield
High thermal and photostability
Moderately hydrophilic
Good solubility in polar solvents
Stable at pH 4 – 11
Cationic dye, perchlorate salt
Molar Mass: 652.2 g/mol

ATTO 633 Datasheet

ATTO 633 Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 629 nm

λ_em = 657 nm

ε_max = 1.3×10⁵

Φ_f = 0.64

τ_fl = 3.2 ns

Brightness = 83.2

Laser = 633 nm

Filter set = Cy®5

ATTO 594

Overview:

High fluorescence yield
High photostability
Very hydrophilic
Excellent solubility in water
Very little aggregation
New dye with net charge of -1
Molar Mass: 1137 g/mol

ATTO 594 Datasheet

ATTO 594 Fluorophore Excitation and Emission Spectrum

Optical Properties:

λ_ex = 601 nm

λ_em = 627 nm

ε_max = 1.2×10⁵

Φ_f = 0.85

τ_fl = 3.5 ns

Brightness = 102

Laser = 594 nm

Filter set = Texas Red®

ATTO 565

Overview:

High fluorescence yield
High thermal and photostability
Good solubility in polar solvents
Excellent solubility in water
Very little aggregation
Rhodamine dye derivative
Molar Mass: 611 g/mol

ATTO 565 Datasheet

ATTO 565 Fluorophore Excitation and Emission Spectra

Optical Properties:

λ_ex = 563 nm

λ_em = 592 nm

ε_max = 1.2×10⁵

Φ_f = 0.9

τ_fl = 3.4 n

Brightness = 10

Laser = 532 nm

Filter set = TRITC

ATTO 488

Overview:

High fluorescence yield
High photostability
Very hydrophilic
Excellent solubility in water
Very little aggregation
New dye with net charge of -1
Molar Mass: 804 g/mol

ATTO 488 Datasheet

ATTO 488 Fluorophore Excitation and Emission Spectra

Optical Properties:

λ_ex = 501 nm

λ_em = 523 nm

ε_max = 9.0×10⁴

Φ_f = 0.80

τ_fl = 4.1 ns

Brightness = 72

Laser = 488 nm

Filter set = FITC

ATTO 390

Overview:

High fluorescence yield
Large Stokes-shift (89 nm)
Good photostability
Moderately hydrophilic
Good solubility in polar solvents
Coumarin derivate, uncharged
Low molar mass: 343.42 g/mol

ATTO 390 Datasheet

ATTO 390 Fluorescent Dye Excitation and Emission Spectra

Optical Properties:

λ_ex = 390 nm

λ_em = 479 nm

ε_max = 2.4×10⁴

Φ_f = 0.90

τ_fl = 5.0 ns

Brightness = 21.6

Laser = 365 or 405 nm

APC (Allophycocyanin)

Overview:

High quantum yield
Large phycobiliprotein
6 chromophores per molecule
Isolated from red algae
Molecular Weight: 105 kDa

APC Datasheet

APC Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 650 nm

λ_em = 660 nm

ε_max = 7.0×10⁵

Φ_f = 0.68

Brightness = 476

Laser = 594 or 633 nm

Filter set = Cy®5

Streptavidin

Properties:

Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
Molecular weight: 53 kDa
Formula: C₁₀H₁₆N₂O₃S
Applications: Western blot, immunohistochemistry, and ELISA

Streptavidin Datasheet

Biotin

Properties:

Binds tetrameric avidin proteins including Streptavidin and neuravidin with very high affinity
Molar mass: 244.31 g/mol
Formula: C₁₀H₁₆N₂O₃S
Applications: Western blot, immunohistochemistry, and ELISA

Biotin Datasheet

HRP (Horseradish peroxidase)

Properties:

Enzymatic activity is used to amplify weak signals and increase visibility of a target
Readily combines with hydrogen peroxide (H₂O₂) to form HRP-H₂O₂ complex which can oxidize various hydrogen donors
Catalyzes the conversion of:
- Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
- Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
- Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
High turnover rate enables rapid generation of a strong signal
44 kDa glycoprotein
Extinction coefficient: 100 (403 nm)
Applications: Western blot, immunohistochemistry, and ELISA

HRP Datasheet

AP (Alkaline Phosphatase)

Properties:

Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
Catalyzes the conversion of:
- Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
- Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
Molecular weight: 140 kDa
Applications: Western blot, immunohistochemistry, and ELISA

AP Datasheet

R-PE (R-Phycoerythrin)

Overview:

Broad excitation spectrum
High quantum yield
Photostable
Member of the phycobiliprotein family
Isolated from red algae
Excellent solubility in water
Molecular Weight: 250 kDa

R-PE Datasheet

R-PE Fluorophore Excitation and Emission Spectra

Optical Properties:

λ_ex = 565 nm

λ_em = 575 nm

ε_max = 2.0×10⁶

Φ_f = 0.84

Brightness = 1.68 x 10³

Laser = 488 to 561 nm

Filter set = TRITC

Properties

Storage Buffer	PBS pH7.4, 50% glycerol, 0.09% sodium azide
Storage Temperature	-20ºC
Shipping Temperature	Blue Ice or 4ºC
Purification	Protein G Purified
Clonality	Monoclonal
Clone Number	S180-41
Isotype	IgG1
Specificity	Detects ~60kDa.
Cite This Product	StressMarq Biosciences Cat# SMC-406, RRID: AB_11232609
Certificate of Analysis	5 µg of SMC-406 was sufficient for immunoprecipitation of EAAC1 from 50 µg of rat brain lysate.

Biological Description

Alternative Names	Excitatory amino-acid carrier 1 Antibody, excitatory amino acid transporter 3 Antibody, EAAT3 Antibody, Neuronal and epithelial glutamate transporter Antibody, Sodium-dependent glutamate/aspartate transporter 3 Antibody, solute carrier family 1 member 1 and Slc1a1 Antibody
Research Areas	Cell Markers, Cell Structure, Membrane Markers, Neuron Markers, Neuroscience, Neurotransmitter Receptors, Pumps/Transporters
Cellular Localization	Membrane
Accession Number	NP_004161.4
Gene ID	6505
Swiss Prot	P43005
Scientific Background	SLC1A1/EAAT3/EAAC1, a member of this transporter family, is expressed throughout the body, particularly in brain, intestine, liver, heart and kidney. Functional defects of the SLC1A1 gene are found in patients of dicarboxylicamino aciduria.
References	1. Smith C. P., et al., (1994) Genomics 20:335-336. 2. Kanai Y., and Hediger M. A. (1992) Nature 360:467-471.

Product Images

<p>Western Blot analysis of Rat brain membrane lysate showing detection of EAAT3 protein using Mouse Anti-EAAT3 Monoclonal Antibody, Clone S180-41 (SMC-406). Primary Antibody: Mouse Anti-EAAT3 Monoclonal Antibody (SMC-406) at 1:1000.</p>

Western Blot analysis of Rat brain membrane lysate showing detection of EAAT3 protein using Mouse Anti-EAAT3 Monoclonal Antibody, Clone S180-41 (SMC-406). Primary Antibody: Mouse Anti-EAAT3 Monoclonal Antibody (SMC-406) at 1:1000.

<p>Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-EAAC1 Monoclonal Antibody, Clone S180-41 (SMC-406). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-EAAC1 Monoclonal Antibody (SMC-406) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Membrane . Magnification: 60X.</p>

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-EAAC1 Monoclonal Antibody, Clone S180-41 (SMC-406). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-EAAC1 Monoclonal Antibody (SMC-406) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Membrane . Magnification: 60X.

Product Citations (0)

Currently there are no citations for this product.

ATTO 390

Overview:

High fluorescence yield
Large Stokes-shift (89 nm)
Good photostability
Moderately hydrophilic
Good solubility in polar solvents
Coumarin derivate, uncharged
Low molar mass: 343.42 g/mol

ATTO 390 Datasheet

Optical Properties:

λ_ex = 390 nm

λ_em = 479 nm

ε_max = 2.4×10⁴

Φ_f = 0.90

τ_fl = 5.0 ns

Brightness = 21.6

Laser = 365 or 405 nm

蚂蚁淘电商平台
ebiomall.com

公司简介

蚂蚁淘（www.ebiomall.cn）是中国大陆目前唯一的生物医疗科研用品B2B跨境交易平台，该平台由多位经验丰富的生物人和IT人负责运营。蚂蚁淘B2B模式是指客户有采购意向后在蚂蚁淘搜索全球供应信息，找到合适的产品后在蚂蚁淘下单，然后蚂蚁淘的海外买手进行跨境采购、运输到中国口岸，最后由蚂蚁淘国内团队报关运输给客户...

蚂蚁淘承诺

正品保证： 全球直采在线追溯蚂蚁淘所有产品都是自运营的，我们已经跟国外多家厂方建立品牌推广合作关系, 获得对方的支持和授权；同时客户可以通过订单详情查看到货物从厂方至客户的所有流程，确保货物的来源；正规报关，提供13%增值税发票。

及时交付： 限时必达畅选无忧蚂蚁淘的运营团队都是有着多年经验的成员，他们熟悉海外采购、仓储物流、报关等环节；同时通过在线的流程监控，蚂蚁淘的进口速度比传统企业提高了50%以上，部分产品甚至能做到7-10天到货，即蚂蚁淘的“时必达”服务。

轻松采购： 在线下单简单省事蚂蚁淘的价格是真实透明的，并且具有很大的价格优势，不需要繁杂的询价比价；报价单与合同可以直接在线生成或打印；就像在京东购物一样，您的鼠标点击几次即完成在蚂蚁淘的采购，订单详情会告诉您所有进程。

售后申请： 耐心讲解优质服务蚂蚁淘提供的产品在使用过程中如因产品质量问题有售后需求时，您可通过我的订单提交您的“申请售后”，蚂蚁淘产品顾问会第一时间为您处理，在售后服务过程中如遇到问题也可致电蚂蚁淘客服热线：4000-520-616。

DNX9620A洗板机性能参数,报价/价格,图片

2019-11-19

焦作路非凡生物科技有限公司在发布的等电聚焦蛋白电泳（IEF）2倍上样缓冲液供应信息，浏览与等电聚焦蛋白电泳（IEF）2倍上样缓冲液相关的产品或在搜索更多与等电聚焦蛋白电泳（IEF）2倍上样缓冲液相关的内容。查看更多>

Random Primers DNA Labeling System

2021-09-12

蛋白电泳上样缓冲液产品名称：蛋白电泳上样缓冲液产品简介：上海通善生物是蛋白电泳上样缓冲液最权威的供应商，提供报价，咨询，技术服务，欢迎来电021-61806666咨询选购。产品库存：现货。产品价格：询价。供应商：通善生物。供应商地址：上海市闵行区金平路788弄166号。蛋白电泳上样缓冲液说明书：蛋白电泳上样缓冲液其它信息：货号产品名称级别K610-1L0.1X SSC BUFFER WITH 0.2% SDS超纯级K611-1L0. 查看更多>

【精品】基质金属蛋白酶抑制剂的研究进展

2021-07-21

2 × Protein Native PAGE Loading Buffer 1 ml × 2支 □ 2 × Protein Native PAGE Loading Buffer组成 Tris-HCl pH8.0 (25℃) 20 mM Glycerol 20% Bromophenol Blue 0.016% 查看更多>

请教上样缓冲液配方中DTT的用法

2021-08-13

货号：WBR0304B 查看更多>

醇溶性漫出物测定法(通则2201)是什么? 药学学术...

2019-02-26

No prestaining "Prestaining" with GRGreen 查看更多>

需要镁离子催化的酶_真核生物的dna聚合酶需要镁离子,这和它的...

2020-01-11

www.biozj.com 查看更多>

酶切实验

2021-07-24

DNA是真核生物的遗传物质，由脱氧核苷酸组成。它是双链互补螺旋结构，定向酶切技术就是用限制性内切酶去切割DNA片断，由于酶具有专一性，一种酶只能识别一种特定的脱氧核苷酸序列，所以可以用特定的这种酶去切割相应的DNA片断，进而达到定向切割的目的。... 查看更多>

RNA干扰技术详解.ppt

2021-08-31

上海研谨生物科技有限公司在发布的普通琼脂糖凝胶RNA/DNA电泳6×上样缓冲液/6×RNA/DNA Loading Buffer供应信息，浏览与普通琼脂糖凝胶RNA/DNA电泳6×上样缓冲液/6×RNA/DNA Loading Buffer相关的产品或在搜索更多与普通琼脂糖凝胶RNA/DNA电泳6×上样缓冲液/6×RNA/DNA Loading Buffer相关的内容。查看更多>

超声波清洗机

2019-01-31

上样缓冲液 DNAII是由上海美吉生物医药科技有限公司代理或销售的Applichem原装品牌的试剂，产品来源于Applichem。上海美吉生物医药科技有限公司是中国最权威的上样缓冲液 DNAII试剂销售服务商之一，在上海等地方销售上样缓冲液 DNAII试剂已经多年。生物在线为您提供众多企业上样缓冲液 DNAII仪器产品及图片，以便挑选到性价比高，合适的上样缓冲液 DNAII产品查看更多>

QuickChange系列突变试剂盒其它资料

2019-05-13

wshtbio 万生昊天的非变性蛋白预制胶属于中性胶。在跑胶之前需要计算蛋白的等电点从而估算蛋白的PH值。如果蛋白PH值小于7，正常跑即可。但如果蛋白的PH大于7呈碱性，就需要将电泳电源正负极倒置。这就带来一个问题，正负极倒置，普通的上样缓冲液里面的溴酚蓝就不会正常自上而下显示电泳进度。这时就需要其他的染料作为指示剂的电泳上样缓冲液。万生昊天现正式推出基于非变性胶测量碱性蛋白时使用的上样缓冲液。能够在电源倒置的... 查看更多>

PCIe插槽 CH225 V3 计算节点 V100R001 用户指南 08 华为

2021-09-18

上海研谨生物科技有限公司在发布的5×DNA Loading buffer with GelRed供应信息，浏览与5×DNA Loading buffer with GelRed相关的产品或在搜索更多与5×DNA Loading buffer with GelRed相关的内容。查看更多>

听说宇通客车用的都是整车电泳、这个整车电泳有什么用处?

2019-10-30

南京赛泓瑞生物科技有限公司在发布的6×RNA/DNA Loading Buffer供应信息，浏览与6×RNA/DNA Loading Buffer相关的产品或在搜索更多与6×RNA/DNA Loading Buffer相关的内容。查看更多>

常见问题

蚂蚁淘所售产品均为正品吗？

蚂蚁淘的创始人兼CEO是钟定松先生，具有十年的从业经验，在业界享有良好的口碑； Ebiomall是跨境直采平台，我们直接从厂家采购，自己的团队负责国际物流和清关，中间没有第三方，蚂蚁淘承诺所售产品仅为正品，假一罚十。

下单后可以修改订单吗？

未确认状态的订单可以修改，打开“订单详情”页面，点击右上角的“修改订单”即可，若已审核确定，则订单无法修改。

商品几天可以发货？

现货产品付款审核后即可发货,大部分期货产品在3周左右即可到货,提供时必达服务的产品订单审核十天内即可发货。

订单如何取消？

如订单处于未确定状态，进入“我的订单"页面，找到要取消的订单，点击“取消订单”按钮。

可以开发票吗？

本网站所售商品都是正规清关，均开具13%正规发票，发票金额含配送费金额，另有说明的除外。

如何联系商家？

蚂蚁淘任何页面都有在线咨询功能，点击“联系客服”、“咨询”或“在线咨询”按钮，均可咨询蚂蚁淘在线客服人员，或拨打4000-520-616，除此之外客户可在联系我们页面找到更多的联系方式。

收到的商品少了/发错了怎么办？

同个订单购买多个商品可能会分为一个以上包裹发出，可能不会同时送达，建议查看订单详情是否是部分发货状态；如未收到，可联系在线客服或者致电4000-520-616。

退换货/维修需要多长时间？

一般情况下，退货处理周期为客户收到产品一个月内（以快递公司显示签收时间为准），包装规格、数量、品种不符，外观毁损、短缺或缺陷，请在收到货24小时内申请退换货；特殊商品以合同条款为准。

商品咨询

4×蛋白上样缓冲液使用说明123

songjunlai19922016-03-22

请问有人知道蛋白上样缓冲液应该怎么配制吗？请赐教

【求助】WB上样缓冲液加加多少是怎么确定的? 蛋白质和糖学...123

你大爷RgZ2017-10-02

上样缓冲液也有多种，一般是80ul的样本加20ul的上样缓冲液，这样基本上上样50ug的体积在10ul左右。
最好是测浓度，一般不超过30-40ug，上样缓冲液一般4×或者5×，这个没太大区别。

5X的SDSPAGE蛋白上样缓冲液配方有谁知道4X的也可以 123

黎约全球の15962017-10-02

total:10ml 4度下保存：
1mol/l Tirs-HCl(PH8.8) 0.6ml
10%SDS 2ml
50%甘油 5ml
2-巯基乙醇 0.5ml
1%溴酚兰 1ml
水 0.9ml
另外：SDS不好溶解建议加热溶解,如果要做非还原SDS-PAGE请将2-巯基乙醇 0.5ml换成超纯水即可

【电泳实验中什么叫上样缓冲液?WESTERNBLOT中需要进行电泳,其具体...123

犬夜叉IQK2017-10-02

蛋白上样缓冲液
蛋白电泳上样缓冲液包括2%SDS , 0.1%溴酚蓝 10%甘油,SDS是保证样品中的所有蛋白带电荷一致，减少电荷对电泳结果的影响。还原剂是让二硫键处于断开状态，保证蛋白分子的线性.
溴酚蓝作用是指示上样蛋白的跑胶时标记的，甘油是增加上样重量的，以免漂浮，煮沸是使蛋白变性的永久保存

请教:2*SDS上样缓冲液的配法蛋白质和糖学论坛123

小2b1雿231k2017-10-02

2× SDS-PAGE 上样缓冲液：
0.5 M tris-HCl (pH 6.8) 20％
丙三醇 20％
20％SDS 20％
0.1％溴酚兰 5％
2-巯基乙醇 10％
双蒸水 25％

不调PH值，PH在配0.5 M tris-HCl 时已调，缓冲液呈蓝紫色。

【求助】western blot上样缓冲液蛋白质和糖学论坛123

woailucy2021-08-02

实验室的一份protocol 上写配上样缓冲液的时候要加溴酚蓝bromophenol blue，但没写加的量是多少。实验室只有粉末状的溴酚蓝。请问大神们如何加，加多少啊？需要先把溴酚蓝配制成溶液吗？还是直接加进去？

4×和5×的上样缓冲液区别| 吉至试剂123

zmp189198708772021-07-31

4×和5×的上样缓冲液区别