| Conjugates | AlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated
| PerCP | Overview:- Peridinin-Chlorophyll-ProteinComplex
- Smallphycobiliprotein
- Isolatedfromredalgae
- Largestokesshift(195nm)
- MolecularWeight:35kDa
PerCPDatasheet |  | OpticalProperties: λex =482nm λem=677nm εmax=1.96x106 Laser =488nm | PE/ATTO594 | | PE/ATTO594isatandemconjugate,wherePEisexcitedat535nmandtransfersenergytoATTO594viaFRET(fluorescenceresonanceenergytransfer),whichemitsat627nm. | Overview:- Highfluorescenceyield
- HighphotostABIlity
- Veryhydrophilic
- Excellentsolubilityinwater
- Verylittleaggregation
PE/ATTO594Datasheet |  | OpticalProperties: λex =535nm λem =627 nm Laser = 488to561nm | FITC(Fluorescein) | Overview:- Excellentfluorescencequantumyield
- Highrateofphotobleaching
- Goodsolubilityinwater
- Broademissionspectrum
- pHdependentspectra
- Molecularformula:C20H12O5
- Molarmass:332.3g/mol
FITC-Fluorescent-conjugate |  | OpticalProperties: λex =494 nm λem=520 nm εmax=7.3×104 Φf= 0.92 τfl =5.0ns Brightness= 67.2 Laser = 488 nm Filterset = FITC | ATTO700 | Overview:- Highfluorescenceyield
- Excellentthermalandphotostability
- Quenchedbyelectrondonors
- Veryhydrophilic
- Goodsolubilityinpolarsolvents
- Zwitterionicdye
- MolarMass:575g/mol
ATTO700Datasheet |  | OpticalProperties: λex =700 nm λem=719nm εmax=1.25×105 Φf= 0.25 τfl =1.6ns Brightness= 31.3 Laser =676nm Filterset =Cy®5.5 | ATTO680 | Overview:- Highfluorescenceyield
- Excellentthermalandphotostability
- Quenchedbyelectrondonors
- Veryhydrophilic
- Goodsolubilityinpolarsolvents
- Zwitterionicdye
- MolarMass:631g/mol
ATTO680Datasheet |  | OpticalProperties: λex =680nm λem=700 nm εmax=1.25×105 Φf= 0.30 τfl =1.7ns Brightness=37.5 Laser =633 –676nm Filterset =Cy®5.5 | ATTO655 | Overview:- Highfluorescenceyield
- Highthermalandphotostability
- Excellentozoneresistance
- Quenchedbyelectrondonors
- Veryhydrophilic
- Goodsolubilityinpolarsolvents
- Zwitterionicdye
- MolarMass:634g/mol
ATTO655Datasheet |  | OpticalProperties: λex =663nm λem=684nm εmax=1.25×105 Φf= 0.30 τfl =1.8ns Brightness= 37.5 Laser =633 –647nm Filterset =Cy®5 | ATTO633 | Overview:- Highfluorescenceyield
- Highthermalandphotostability
- Moderatelyhydrophilic
- Goodsolubilityinpolarsolvents
- StableatpH4–11
- Cationicdye,perchloratesalt
- MolarMass:652.2g/mol
ATTO633Datasheet |  | OpticalProperties: λex =629nm λem=657nm εmax=1.3×105 Φf= 0.64 τfl =3.2ns Brightness=83.2 Laser =633 nm Filterset =Cy®5 | ATTO594 | Overview:- Highfluorescenceyield
- Highphotostability
- Veryhydrophilic
- Excellentsolubilityinwater
- Verylittleaggregation
- Newdyewithnetchargeof-1
- MolarMass:1137g/mol
ATTO594Datasheet |  | OpticalProperties: λex =601 nm λem=627nm εmax=1.2×105 Φf= 0.85 τfl =3.5ns Brightness=102 Laser =594nm Filterset =TexasRed® | ATTO565 | Overview:- Highfluorescenceyield
- Highthermalandphotostability
- Goodsolubilityinpolarsolvents
- Excellentsolubilityinwater
- Verylittleaggregation
- Rhodaminedyederivative
- MolarMass:611g/mol
ATTO565Datasheet |  | OpticalProperties: λex =563nm λem=592nm εmax=1.2×105 Φf= 0.9 τfl =3.4n Brightness=10 Laser =532nm Filterset = TRITC | ATTO488 | Overview:- Highfluorescenceyield
- Highphotostability
- Veryhydrophilic
- Excellentsolubilityinwater
- Verylittleaggregation
- Newdyewithnetchargeof-1
- MolarMass:804g/mol
ATTO488Datasheet |  | OpticalProperties: λex =501nm λem=523nm εmax=9.0×104 Φf= 0.80 τfl =4.1ns Brightness= 72 Laser = 488 nm Filterset = FITC | ATTO390 | Overview:- Highfluorescenceyield
- LargeStokes-shift(89nm)
- Goodphotostability
- Moderatelyhydrophilic
- Goodsolubilityinpolarsolvents
- Coumarinderivate,uncharged
- Lowmolarmass:343.42g/mol
ATTO390Datasheet |  | OpticalProperties: λex =390nm λem=479nm εmax=2.4×104 Φf= 0.90 τfl =5.0ns Brightness= 21.6 Laser =365or405nm | APC(Allophycocyanin) | Overview:- Highquantumyield
- Largephycobiliprotein
- 6chromophorespermolecule
- Isolatedfromredalgae
- MolecularWeight:105kDa
APCDatasheet |  | OpticalProperties: λex =650nm λem=660nm εmax=7.0×105 Φf= 0.68 Brightness= 476 Laser =594or633 nm Filterset =Cy®5 StreptavidinProperties: - Homo-tetramericproteinpurifiedfromStreptomycesavidiniiwhichbindsfourbiotinmoleculeswithextremelyhighaffinity
- Molecularweight:53kDa
- Formula:C10H16N2O3S
- Applications:Westernblot,immunohistochemistry,andELISA
StreptavidinDatasheet Biotin Properties: - BindstetramericavidinproteinsincludingStreptavidinandneuravidinwithveryhighaffinity
- Molarmass:244.31g/mol
- Formula:C10H16N2O3S
- Applications:Westernblot,immunohistochemistry,andELISA
BiotinDatasheet HRP(HorserADIshperoxidase)Properties: - Enzymaticactivityisusedtoamplifyweaksignalsandincreasevisibilityofatarget
- Readilycombineswithhydrogenperoxide(H2O2)toformHRP-H2O2complexwhichcanoxidizevarioushydrogendonors
- Catalyzestheconversionof:
- Chromogenicsubstrates(e.g.TMB,DAB,ABTS)intocolouredproducts
- Chemiluminescentsubstrates(e.g.luminolandisoluminol)intolightemittingproductsviaenhancedchemiluminescence(ECL)
- Fluorogenicsubstrates(e.g.tyramine,homovanillicacid,and4-hydroxyphenylaceticacid)intofluorescentproducts
- Highturnoverrateenablesrapidgenerationofastrongsignal
- 44kDaglycoprotein
- Extinctioncoefficient:100(403nm)
- Applications:Westernblot,immunohistochemistry,andELISA
HRPDatasheet AP(AlkalinePhosphatase)Properties: - Broadenzymaticactivityforphosphateestersofalcohols,amines,pyrophosphate,andphenols
- Commonlyusedtodephosphorylatethe5’-terminiofDNAandRNAtopreventself-ligation
- Catalyzestheconversionof:
- Chromogenicsubstrates(e.g.pNPP,naphtholAS-TRphosphate,BCIP)intocolouredproducts
- Fluorogenicsubstrates(e.g.4-methylumbelliferylphosphate)intofluorescentproducts
- Molecularweight:140kDa
- Applications:Westernblot,immunohistochemistry,andELISA
APDatasheet | R-PE(R-Phycoerythrin) | Overview:- Broadexcitationspectrum
- Highquantumyield
- Photostable
- Memberofthephycobiliproteinfamily
- Isolatedfromredalgae
- Excellentsolubilityinwater
- MolecularWeight:250kDa
R-PEDatasheet |  | OpticalProperties: λex =565nm λem=575nm εmax=2.0×106 Φf= 0.84 Brightness=1.68x103 Laser = 488to561nm Filterset = TRITC ProductImages WesternblotanalysisofRatkidneytissuelysatesshowingdetectionofENaCproteinusingRabbitAnti-ENaCPolyclonalAntibody(SPC-403).PrimaryAntibody:RabbitAnti-ENaCPolyclonalAntibody(SPC-403)at1:1000.  WesternblotanalysisofMousekidneytissuelysatesshowingdetectionofENaCproteinusingRabbitAnti-ENaCPolyclonalAntibody(SPC-403).PrimaryAntibody:RabbitAnti-ENaCPolyclonalAntibody(SPC-403)at1:1000. ProductCitations(34)WesternBlotEnacRegulationintheKidney:theRoleofAnkyrinG Klemens,C.A.(2017)UniversityofPittsburgh.PhDDissertation. PubMedID:N/AReactivityRatApplications:WesternBlot Derlin-1promotesubiquitinationanddegradationofepithelialsodiumchannel(ENaC). You,H.etal.(2017)JCellSci.130(6):1027-1036. PubMedID:28137758ReactivityMouseApplications:WesternBlot Derlin-1promotesubiquitinationanddegradationofepithelialsodiumchannel(ENaC). You,H.etal.(2017)JCellSci.130(6):1027-1036. PubMedID:28137758ReactivityHumanApplications:WesternBlot AnkyrinGExpressionRegulatesApicalDeliveryoftheEpithelialSodiumChannel(ENaC). Klemens,C.A.,Edinger,R.S.,Kightlinger,L.,Liu,X.andButterworth,M.B.(2016)JBiolChem.292(1):375-385. PubMedID:27895120ReactivityMouseApplications:WesternBlot Renalsodiumtransportinrenin-deficientDahlsalt-sensitiverats. Pavlov,T.S.,Levchenko,V.,Ilatovskaya,D.V.,Moreno,C.andStaruschenko,A.(2016)JReninAngiotensinAldosteroneSyst.17(3). PubMedID:27443990ReactivityRatApplications:WesternBlot (Pro)ReninReceptorRegulatesPotassiumHomeostasisthroughaLocalMechanism. Xu,C.etal.(2016)AmJPhysiolRenalPhysiol.[Epubaheadofprint] PubMedID:27440776ReactivityRatApplications:WesternBlot AMP-activatedproteinkinaseattenuateshighsalt-inducedactivationofepithelialsodiumchannels(ENaC)inhumanumbilicalveinendothelialcells. Zheng,W.etal.(2016)OxidMedCellLongev.2016:1531392. PubMedID:27635187ReactivityHumanApplications:WesternBlot Renaltubularepithelialcellproreninreceptorregulatesbloodpressureandsodiumtransport. Ramkumar,N.etal.(2016)AmJPhysiolRenalPhysiol.311(1):F186-94. PubMedID:27053687ReactivityMouseApplications:WesternBlot TheRoleofEpithelialSodiumChannelENaCandtheApicalCl-/HCO3-ExchangerPendrininCompensatorySaltReabsorptionintheSettingofNa-ClCotransporter(NCC)Inactivation. Patel-Chamberlin,M.etal.(2016)PLoSOne.11(3):e0150918. PubMedID:26963391ReactivityMouseApplications:WesternBlot PossIBLerolefornephron-derivedangiotensinogeninangiotensin-IIdependenthypertension. Ramkumar,N.etal.(2016)PhysiolRep.4(1).pii:e12675. PubMedID:26755736ReactivityMouseApplications:WesternBlot TheThiazide-SensitiveCo-TransporterPromotestheDevelopmentofSodiumRetentioninMicewithDiet-InducedObesity. Davies,M.R.P.etal.(2015)KidneyBloodPressRes.40(5):509-519. PubMedID:26418861ReactivityMouseApplications:WesternBlot InhibitionofMitochondrialComplex-1PreventstheDownregulationofNKCC2andENaCαinObstructiveKidneyDisease. Zhang,Y.etal.(2015)SciRep.5:12480. PubMedID:26207612ReactivityMouseApplications:WesternBlot IncreasedEpithelialSodiumChannelActivityContributestoHypertensionCausedbyNa+-HCO3−CotransporterElectrogenic2Deficiency. Wen,D.etal.(2015)Hypertension.66(1):68-74. PubMedID:25941340ReactivityMouseApplications:WesternBlot CompositionsAndMethodsOfUseFor(PRO)ReninReceptor. Feng,Y.andYang,T.(2015)UnitedStatesPatentApplication20150025013. PubMedID:N/AReactivityRatApplications:WesternBlot RoleofRhoGDP-dissociationinhibitoralphaincontrolofENaC-mediatedsodiumreabsorption. Pavlov,T.S.,Levchenko,V.andStaruschenko,A.(2014)JBiolChem.289(41):28651-9. PubMedID:25164814ReactivityMouseApplications:WesternBlot Collectingductspecificknock-outofreninattenuatesangiotensin-IIinducedhypertension. Ramkumar,N.etal.(2014)AmJPhysiolRenalPhysiol.307(8):F931-8. PubMedID:25122048ReactivityMouseApplications:WesternBlot NovelMechanismsofSodiumRetentioninObesity:PhosphorylationofNKCC2andRegulationofSPAK/OSR1byAMPK. Davies,M.R.P.etal.(2014)AmJPhysiol.307(1):F96-F106. PubMedID:24808538ReactivityMouseApplications:WesternBlot ChronicAngiotensinIIInfusionDrivesExtensiveAldosterone-IndependentEpithelialNa+ChannelActivation. Mamenko,M.et.al.(2013)Hypertension.62(6):1111-22. PubMedID:24060890ReactivityMouseApplications:WesternBlot AdenylylCyclaseVIMediatesVasopressin-StimulatedENaCActivity. Roos,K.P.etal.(2012)JASN.24(2):218-227. PubMedID:23264685ReactivityMouseApplications:WesternBlot IncreasedexpressionofrenalTRPM6compensatesforMg2+wastingduringfurosemidetreatment. vanAngelen,A.A.,Kemp,A.W.,Hoenderop,J.G.andBindels,B.J.(2012)ClinKidneyJ.5(6):535-544. PubMedID:26069797ReactivityMouseApplications:WesternBlot Regionaldifferencesinratconjunctivaliontransportactivities. Yu,D.etal.(2012)AmJPhysiolCellPhysiol.303(7):767-780. PubMedID:22814399ReactivityXenopuslaevisApplications:WesternBlot TheEpithelialSodiumChannel(ENaC)EstablishesaTraffickingVesiclePoolResponsibleforItsRegulation. Edinger,R.S.etal.(2012)PLoSONE.7(9):e46593. PubMedID:23029554ReactivityMouseApplications:WesternBlot AldosteronedoesnotrequireangiotensinIItoactivateNCCthroughaWNK4–SPAK–dependentpathway. vanderLubbe,N.etal.(2012)EuroJPhysiol.463(6):853-863. PubMedID:22549242ReactivityRatApplications:WesternBlot ImmunohistochemistryTheProstaglandinE2Receptor1(EP1)AntagonizesAngIIintheCollectingDuct. Eckert,D.(2017)UniversityofOttawa.Dissertation. PubMedID:N/AReactivityMouseApplications:Immunohistochemistry InhibitionofMitochondrialComplex-1PreventstheDownregulationofNKCC2andENaCαinObstructiveKidneyDisease. Zhang,Y.etal.(2015)SciRep.5:12480. PubMedID:26207612ReactivityMouseApplications:Immunohistochemistry EpoxyeicosatrienoicAcid(EET)AnalogLowersBloodPressureThroughVasodilationAndSodiumChannelInhibition. Khan,M.A.,etal.(2014)ClinSci(Lond).127(7):463-74. PubMedID:24707975ReactivityRatApplications:Immunohistochemistry ChronicAngiotensinIIInfusionDrivesExtensiveAldosterone-IndependentEpithelialNa+ChannelActivation. Mamenko,M.et.al.(2013)Hypertension.62(6):1111-22. PubMedID:24060890ReactivityMouseApplications:Immunohistochemistry ENaCγ-expressingastrocytesinthecircumventricularorgans,whitematter,andventralmedullarysurface:sitesforNa+regulationbyglialcells. Miller,R.L.andLoewy,A.D.(2013)JChemNeuroanat.53:72-80. PubMedID:24145067ReactivityRatApplications:Immunohistochemistry RegulationofENaCinmicelackingrenalinsulinreceptorsinthecollectingduct. Pavlov,T.S.et.al.(2013)FASEBJ.27(7):2723-32. PubMedID:23558339ReactivityMouseApplications:Immunohistochemistry OtherCitationsDerlin-1promotesubiquitinationanddegradationofepithelialsodiumchannel(ENaC). You,H.etal.(2017)JCellSci.130(6):1027-1036. PubMedID:28137758ReactivityMouseApplications:Immunoprecipitation Derlin-1promotesubiquitinationanddegradationofepithelialsodiumchannel(ENaC). You,H.etal.(2017)JCellSci.130(6):1027-1036. PubMedID:28137758ReactivityHumanApplications:Immunoprecipitation ENaCandROMKactivityareinhibitedintheDCT2/CNTofTgWnk4PHAIImice. Zhang,C.etal.(2016)AmJPhysiolRenalPhysiol.[Epubaheadofprint] PubMedID:N/A mTORC1maintainsrenaltubularhomeostasisandisessentialinresponsetoischemicstress. Grahammer,F.etal.(2014)ProcNatlAcadSciUSA.111(27):E2817-26. PubMedID:24958889ReactivityMouseApplications:GelShift mPGES-1-DerivedPGE2MediatesDehydrationNatriuresis. Jia,Z.etal.(2013)AmJPhysiolRenalPhysiol.304(2):F214-21. PubMedID:23171554ReactivityMouseApplications:WesternBlot | ATTO488 | Overview:- Highfluorescenceyield
- Highphotostability
- Veryhydrophilic
- Excellentsolubilityinwater
- Verylittleaggregation
- Newdyewithnetchargeof-1
- MolarMass:804g/mol
ATTO488Datasheet | | OpticalProperties: λex =501nm λem=523nm εmax=9.0×104 Φf= 0.80 τfl =4.1ns Brightness= 72 Laser = 488 nm Filterset = FITC
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焦作路非凡生物科技有限公司在发布的等电聚焦蛋白电泳（IEF）2倍上样缓冲液供应信息，浏览与等电聚焦蛋白电泳（IEF）2倍上样缓冲液相关的产品或在搜索更多与等电聚焦蛋白电泳（IEF）2倍上样缓冲液相关的内容。 查看更多>
蛋白电泳上样缓冲液产品名称：蛋白电泳上样缓冲液产品简介：上海通善生物是蛋白电泳上样缓冲液最权威的供应商，提供报价，咨询，技术服务，欢迎来电021-61806666咨询选购。产品库存：现货。产品价格：询价。供 应 商：通善生物。供应商地址：上海市闵行区金平路788弄166号。蛋白电泳上样缓冲液说明书：蛋白电泳上样缓冲液其它信息：货号产品名称级别K610-1L0.1X SSC BUFFER WITH 0.2% SDS超纯级K611-1L0. 查看更多>
2 × Protein Native PAGE Loading Buffer 1 ml × 2支
□ 2 × Protein Native PAGE Loading Buffer组成
Tris-HCl pH8.0 (25℃) 20 mM
Glycerol 20%
Bromophenol Blue 0.016% 查看更多>
No prestaining "Prestaining" with GRGreen 查看更多>
DNA是真核生物的遗传物质，由脱氧核苷酸组成。它是双链互补螺旋结构，定向酶切技术就是用限制性内切酶去切割DNA片断，由于酶具有专一性，一种酶只能识别一种特定的脱氧核苷酸序列，所以可以用特定的这种酶去切割相应的DNA片断，进而达到定向切割的目的。... 查看更多>
上海研谨生物科技有限公司在发布的普通琼脂糖凝胶RNA/DNA电泳6×上样缓冲液/6×RNA/DNA Loading Buffer供应信息，浏览与普通琼脂糖凝胶RNA/DNA电泳6×上样缓冲液/6×RNA/DNA Loading Buffer相关的产品或在搜索更多与普通琼脂糖凝胶RNA/DNA电泳6×上样缓冲液/6×RNA/DNA Loading Buffer相关的内容。 查看更多>
上样缓冲液 DNAII是由上海美吉生物医药科技有限公司代理或销售的Applichem原装品牌的试剂，产品来源于Applichem。上海美吉生物医药科技有限公司是中国最权威的上样缓冲液 DNAII试剂销售服务商之一，在上海等地方销售上样缓冲液 DNAII试剂已经多年。生物在线为您提供众多企业上样缓冲液 DNAII仪器产品及图片，以便挑选到性价比高，合适的上样缓冲液 DNAII产品 查看更多>
wshtbio 万生昊天的非变性蛋白预制胶属于中性胶。 在跑胶之前需要计算蛋白的等电点从而估算蛋白的PH值。 如果蛋白PH值小于7， 正常跑即可。 但如果蛋白的PH大于7呈碱性， 就需要将电泳电源正负极倒置。 这就带来一个问题， 正负极倒置， 普通的上样缓冲液里面的溴酚蓝就不会正常自上而下显示电泳进度。 这时就需要其他的染料作为指示剂的电泳上样缓冲液。 万生昊天现正式推出基于非变性胶测量碱性蛋白时使用的上样缓冲液。 能够在电源倒置的... 查看更多>
上海研谨生物科技有限公司在发布的5×DNA Loading buffer with GelRed供应信息，浏览与5×DNA Loading buffer with GelRed相关的产品或在搜索更多与5×DNA Loading buffer with GelRed相关的内容。 查看更多>
南京赛泓瑞生物科技有限公司在发布的6×RNA/DNA Loading Buffer供应信息，浏览与6×RNA/DNA Loading Buffer相关的产品或在搜索更多与6×RNA/DNA Loading Buffer相关的内容。 查看更多>
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上样缓冲液也有多种，一般是80ul的样本加20ul的上样缓冲液，这样基本上上样50ug的体积在10ul左右。
最好是测浓度，一般不超过30-40ug，上样缓冲液一般4×或者5×，这个没太大区别。
total:10ml 4度下保存：
1mol/l Tirs-HCl(PH8.8) 0.6ml
10%SDS 2ml
50%甘油 5ml
2-巯基乙醇 0.5ml
1%溴酚兰 1ml
水 0.9ml
另外：SDS不好溶解建议加热溶解,如果要做非还原SDS-PAGE请将2-巯基乙醇 0.5ml换成超纯水即可
蛋白上样缓冲液
蛋白电泳上样缓冲液包括2%SDS , 0.1%溴酚蓝 10%甘油,SDS是保证样品中的所有蛋白带电荷一致，减少电荷对电泳结果的影响。还原剂是让二硫键处于断开状态，保证蛋白分子的线性.
溴酚蓝作用是指示上样蛋白的跑胶时标记的，甘油是增加上样重量的，以免漂浮，煮沸是使蛋白变性的永久保存
2× SDS-PAGE 上样缓冲液：
0.5 M tris-HCl (pH 6.8) 20％
丙三醇 20％
20％SDS 20％
0.1％溴酚兰 5％
2-巯基乙醇 10％
双蒸水 25％
不调PH值，PH在配0.5 M tris-HCl 时已调，缓冲液呈蓝紫色。
实验室的一份protocol 上写配上样缓冲液的时候要加溴酚蓝bromophenol blue，但没写加的量是多少。实验室只有粉末状的溴酚蓝。请问大神们如何加，加多少啊？需要先把溴酚蓝配制成溶液吗？还是直接加进去？
同仁好，我想请教一下大家，6Xloddingbuffer1% 琼脂糖凝胶电泳TBE 缓冲液，5ul DNA样品和1ul 上样缓冲液怎么就是跑不出主带啊？都是弥散带，给点建议我哪里有问题啊？
不太好操作，里面有小分子的SDS、β巯基乙醇，较难浓缩，不是有4X的买么，还可以自己配啊，网上都有现成的配方，溴酚蓝、SDS、β巯基乙醇。。。
急求20mmol/L，pH5的柠檬酸磷酸盐 缓冲液如何配制？？？需要详细的配置过程，分别需要柠檬酸和磷酸氢二钠多少克？？？谢谢!!!!缓冲液是做为 上样缓冲液用的，谢谢大神们给予一些指点
作为指示剂，因为溴酚蓝呈蓝色，而蛋白质是白色，在SDS-凝胶中不明显，且溴酚蓝的相对分子量比蛋白质(绝大部分)小，电泳时速度比蛋白质稍快，因此当溴酚蓝到达电泳槽底部(可看蓝色调带)，则电泳结束。
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