Type I collagen is a major structural component of skin, bone, tendon, and other fibrous connective tissues, and differs from other collagens by its low lysine hydroxylation and low carbohydrate composition. Although a number of types of collagen have been identified, all are composed of molecules containing three polypeptide chains arranged in a triple helical conformation. Slight differences in the primary structure (amino acid sequence) establish differences between the types.
This product is suitable for the preparation of collagen gels for the culture of human hematopoietic progenitor cells. This product can also be used to prepare collagen films (collagen coating) on cell culture surfaces.
The rate of gel formation, gel consistency and gel clarity may vary between lots. Agitation of the gel during formation, exposure to ultraviolet light and temperature extremes can influence the integrity of the gel.
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配制分三步: 1) 1 M Tris-HCl (pH 8.0) 50 ml的配制:称取Tris碱6.06 g,加超纯水40 ml溶解,滴加浓HCl约2.1 ml调pH至8.0,定容至50 ml。
电泳缓冲液的另一个作用是使溶液具有一定的导电性,以利于DNA分子的迁移,例如,一般电泳缓冲液中应含有0.01-0.04mol/L的Na+离子,Na+离子的浓度太低时电泳速度变慢;太高时就会造成过大的电流使胶发热甚至熔化。
电泳缓冲液还有一个组分是EDTA,加入浓度为1-2mmol/L,目的是螯合Mg2+ 等离子,防止电泳时激活 DNA酶,此外还可防止Mg2+离子与核酸生成沉淀。

