DescriptionViPrimePLUS Taq qPCR Master Mix is next generation master mix designed for fast and easy realtime PCR reaction set up. The master mix is prepared in 2X concentrated solution and contains all the reagents, including pure Taq DNA Polymerases, highest quality dNTPs as well as buffer components at optimal concentrations, except for template, primers and probes. Taq DNA Polymerases in the master mix provide excellent results in reaction efficiency, correlation coefficient and slope.ViPrimePLUS Taq qPCR Master Mix can be used to amplify any DNA template including genomic, cDNA and viral sequences. The formulation of qPCR master mix can detect low copy number targets very specifically with high efficiency. The qPCR master mix provides convenient and robust set up for quantitative real-time analysis of DNA samples.ViPrimePLUS Taq qPCR Master Mix has several formulations optimized to be used with most of realtime PCR instruments. The sensitivity and consistency of ViPrimePLUS Taq qPCR Master Mix in standard cycling conditions gives the industry leading performance in fast cycling conditions.
ApplicationsAll kinds of sample material suited for qPCR amplification can be used.
Features
- Ready-to-use real-time PCR reaction set up
- Rapid extension rate for early Ct values
- Good buffer system for excellent amplification efficiency
- High sensitivity detection
- Compatible with most of the real-time PCR platforms
Component1.6ml aliquots of master mix
StorageStable at -20°C up to the expiry date stated. Store all components at -20°C upon arrival. Keep in aliquot to reduce freeze-thaw cycles.
Quality ControlAs part of the ISO9001:2008 quality assurance systems, each lot of ViPrimePLUS Taq qPCR Master Mix has been tested against predetermined specifications to ensure consistent product quality and highest levels of performance and reliability.
Limitation Of UseFor research use only. Not recommended or intended for diagnosis of disease in humans or animals. Do not use internally or externally in humans or animals.
InstrumentsTo calibrate a real-time PCR reaction, various formulations of master mixes are available for most of the platforms.
| Product Description | Compatible Hardware |
| QLMM11ViPrimePLUS Taq qPCR Master Mix | Biometra qTower, BioRad iCycler, BioRad IQ4, BioRad IQ5, Cepheid SmartCycler®, Eppendorf Mastercycler, Fluidigm BioMark™, Illumina Eco, MJ Chromo4, Opticon, PCRMax Eco™, Roche lightcycler® 480, lightcycler® LC96 and lightcycler® Nano Platforms, RotorGene, Roche Capillary Lightcycler 1.0-2.0, Stratagene MX MX4000P®, MX3000P®, MX3005®, Thermo PikoReal™ |
| QLMM11-LRViPrimePLUS Taq qPCR Master Mix with Low ROX | Applied Biosystems 7500 and 7500 FAST platform, QuantStudio™, ViiA7 |
| QLMM11-RViPrimePLUS Taq qPCR Master Mix with ROX | Applied Biosystems 7000, 7300, 7700, 7900 and 7900HT FAST platforms, GeneAmp® 5700, StepOne™, StepOne™ PLUS |
Ordering Information
| Catalog No | Description | Pack Size |
| QLMM11 | ViPrimePLUS Taq qPCR MasterMix | 150 reactions |
| QLMM11-LR | ViPrimePLUS Taq qPCR MasterMix with Low ROX | 150 reactions |
| QLMM11-R | ViPrimePLUS Taq qPCR MasterMix with ROX | 150 reactions |
DownloadManual
ViPrimePLUS Taq qPCR MasterMix ViPrimePLUS Taq qPCR MasterMix with Low ROX ViPrimePLUS Taq qPCR MasterMix with ROX
PublicationThis Product Has Been Used In: Amompan, P., Petthai, A., Monthatong, M., Lomthaisong, K. (2016).Human DNA Identification and Sex Determination from Bloodstains by Duplex PCR Analysis Asia-Pacific Journal of Science and Technology, Vol. 22, No. 4 (2016).
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采用OPA柱前衍生，波长是338nm
流动相A:20mmol/LNaH2PO4(用10mol/LNaOH溶液调pH至7.8±0.2);
流动相B:甲醇:乙腈:水=(45:45:10，V/V/V);
柱温:40℃;
然后进其他单标氨基酸的时候都没有问题，但是进单标胱氨酸的时候，HPLC色谱图中出了两峰，为什么会有两峰？这两峰分别是什么峰？其中一个是半胱氨酸的峰吗？用何种方法可以确定这两个分别是什么峰？
20种蛋白质氨基酸都是α氨基酸。
其中除了甘氨酸外，其他氨基酸都具有旋光性，旋光性是由分子的空间构象引起的，你可以理解成像你的手掌一样，左右手的指头位置不同导致左右手的区别。根据旋光性分为L型（左型）和D型（右型）两种。
组成生命的氨基酸基本上都是L型。
第一次发帖，生怕叙述不清。
请问有哪位大神做过几个同源基因氨基酸序列保守性的图，本人做了一篇几个同源基因的SNP与疾病相关性的研究，投稿后，reviewer给的意见是，希望做一副这几个同源基因的氨基酸序列比对结果图，标注保守序列，我会查，但是不知道怎么做图，截屏么？还是有什么专用的软件？谢谢大家！
类似这样，但这个图是不同物种的同一基因序列保守性分析的图
在一些解热镇痛药注射剂中，为避免药物发黄变色，又加入了氨基酸抗氧化剂（除了亚硫酸氢钠外）。氨基酸抗氧化剂有哪些呢，常用的。查了一下，含S的抗氧化剂有甲硫氨酸(Met)和半胱氨酸(Cys)，是这些吗？还是有其他常用的呢？
没有这8种氨基酸就会发生由于缺乏营养所引起的疾病;氨基酸在医药上也有很大的用途，现在手术中输液都加有各种氨基酸，使患者的抵抗力增强，同时也增加营养。随着人民生活水平的提高，人们对摄入的营养物质的要求越来越高，尤其是幼儿、青少年的健康成长，疾病患者的康复，都迫切需要高质量的营养物质，所以有效开发氨基酸食品是很有必要的。
