Description
TheGly-X™N-GlycanRapidReleaseandLabelingwithInstantPC™kitutilizesanovelin-solutionenzymaticproteindeglycosylationfollowedbyrapidlabelingofreleasedN-glycanswithInstantPCdye.Afterasimpleclean-upstep,theglycansamplesarereadyforanalysisbyUHPLC,LC-MS,MS/MSandothermethods.Withdeglycosylationandlabelingcarriedoutinsolution,themethodissimple,rapidandsuitableforautomation.TheInstantPCdyedeliversunmatchedfluorescentbrightnessandMSsignal,whichenablesasinglelabelingmethodtobedeployedacrossdifferentglycananalysisworkflows.
Samplepreptime: ~1hour.
Otherbenefitsinclude:
- Smallmoleculesizeforimprovedchromatographicpeakresolution
- FlexIBLe,high-throughputformat:process1to24 samples
- Optimizedcleanupremovesexcessfreedye,protein,andotherinterferingcompounds
- ReconstitutedInstantPCdyemaybestored frozen(-20°C)forupto1month
Contains:
- GX24-100Gly-XDeglycosylationModule
- GX24-101Gly-XInstantPCLabelingModule
- GX96-102Gly-XInstantPCClean-upModule
- Note:The24-countkitincludesa96-countcleanupplate.Thisplatecanbestoredatroomtemperatureforfutureuse.Gly-XInstantPCdigestionandlabelingreagents(GX24-201PC) areavailableseparatelyforusewithexistingcleanupplates.
Equipmentandreagentsprovidedbyuser:
- 96-wellThermocyclerortwoindependentheatblocks,setfor90°Cand50°C
- Thermocycler(CorningTHERM-1001,110V;THERM-1000,230V)
- Note:TwoGlykoPrepHeaters,WS0271,canbefittedwithVWR13259-260ModularHeatingBlocks
- Vacuummanifold(MilliporeMSVMHTS00-HTS)
- Vacuumpump(MilliporeWP6211560,110V;WP6122050,220V;WelchWOB-LPump2522)
- Formicacid,MS-grade(FisherA117-50recommended)
- Acetonitrile(ACN),MS-grade(FisherA955-4recommended)
Size:1kit(24-ct)
ProductCode:GX24-IPC
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还有如果用气相做糖的鉴别的话,需要样品量大不大?怎么衍生化?
不好意思,我是学药分的,对植化比较外行,请详细些,谢了先!
一般情况下同时测定蔗糖、果糖和葡萄糖。
选用哪种检测器呐。
本来经战友的意见已决定用示差了,但突然有个经销商愿意以比较优惠的价格卖蒸发光给我们,所以又开始犹豫了。
最后一次请教各位战友,如题目。谢谢!!
1.CA15-3属于多形上皮黏蛋白。CA15-3测定广泛用于乳腺癌的辅助诊断。60%~80%进展期乳腺癌患者的CA15-3浓度大30kU/L。CA15-3有助于判断肿瘤的进展和疗效,测定值大于1000kU/L者预后不良。在乳腺癌初期其灵敏度较低,在Ⅲ期时约为12%,因此CA15-3测定不能用作乳腺癌的筛选手段.当怀疑乳腺癌而CA15-3又正常时,可以结合CEA值进行考虑。应注意到治疗后由于肿瘤细胞死亡,CA15-3起初会升高,然后才逐渐下降。持续不降或上升者则提示病情恶化。
2.其他恶性肿瘤,如肺、结肠、胰腺、肝脏、卵巢癌、子宫颈和子宫内膜的恶性肿瘤也都可见到CA15-3不同程度的阳性率(低于10%)。
3.肝硬化、肝炎、结节病、结核病、自身免疫性疾病(SLE)以及卵巢、乳腺的良性病变中,CA15-3值略高于正常范围,但阳性率一般低于10%,妊娠中后期,有近一半孕妇血清CA15-3升高。
核酸是天然高分子化合物,,糖类中的多糖(淀粉、纤维素、糖原等)也是天然高分子化合物
同属于生命的六大营养物质(水,无机盐,糖类,脂质,核酸,蛋白质)

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