Details
Pvu I Source
Proteus vulgaris
Reagents Supplied
CMX Buffer 3
Unit Definition
One unit is the amount of enzyme required to completely digest 1 µg of Adenovirus 2 DNA in 1 hour at 37°C in a total reaction volume of 50 µl.
Star Activity
No
Incubation Temperature
37°C
Heat Inactivation
10 minutes at 80°C
Assay Unit Substrate
Adenovirus 2 DNA
Reaction Buffer
CMX Buffer 3;
50 mM Tris-HCl (pH 7.4 at 37°C)
10 mM MgCl2
100 mM NaCl2
1 mM dithiothreitol
100 µg/ml bovine serum albumin
Storage Buffer
50mM Tris-HCl (pH 7.5 at 4°C)
200 mM KCl
0.1 mM EDTA
10mM β-mercaptoethanol
0.2% (v/v) Triton X-100™
500 µg/ml bovine serum albumin
50% (v/v) glycerol
Assay Conditions
50 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl2, 100 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin
1 µg Adenovirus 2 DNA
Reaction volume 50 µl
Storage Conditions
Store at -20°C
Shipped on dry ice
Pvu I Downloads
Certificate of Analysis PDF -Current Lot
MSDS PDF -Current Lot
Categories
Restriction Endonucleases, Pvu I
ebiomall.com
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