Details
SacISource
Streptomycesachromogenes
ReagentsSupplied
CMXBuffer2
UnitDefinition
Oneunitistheamountofenzymerequiredtocompletelydigest1µgofAdenovirus2DNAin1hourinatotalreactionvolumeof50 µl
StarActivity
Yes
IncubationTemperature
37°C
HeatInactivation
10minutesat65°C
AssayUnitSubstrate
Adenovirus2DNA
ReactionBuffer
10mMTris-HCl(pH7.5at37°C)
10mMMgCl2
50mMNaCl
1mMdithiothreitol
100µg/mlbovineserumalbumin-Seemoreat:http://www.Chimerx.com/store/restriction-endonucleases/hinfi#sthash.eTrsWtsh.dpuf
CMXBuffer2:
10mMTris-HCl(pH7.5at37°C)
10mMMgCl2
50mMNaCl
1mMdithiothreitol
100µg/mlbovineserumalbumin
10mMTris-HCl(pH7.5at37°C)
10mMMgCl2
50mMNaCl
1mMdithiothreitol
100µg/mlbovineserumalbumin-Seemoreat:http://www.chimerx.com/store/restriction-endonucleases/hinfi#sthash.eTrsWtsh.dpuf
StorageBuffer
20mMTris‑HCl(pH8.0at4ºC)
150mMKCl
500µg/mlbovineserumalbumin
0.1%TritonX-100™
50%(v/v)glycerol
AssayConditions
10mMTris‑HCl(pH7.5at37ºC)
10mMMgCl2
1mMdithiothreitol
1µgAdenovirus2DNA
Incubationisat37°Cfor1hourinatotalreactionvolumeof50µl
StorageConditions
Storeat-20°C
Shippedondryice
SacIDownloads
CertificateofAnalysisPDF -CurrentLot
MSDSPDF -CurrentLot
Categories
Restrictionendonucleases
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希望同路人多多交流啊!
我想用共聚焦观察间期染色体,用涂染探针,有一个问题很困扰我,我想涂染完染色后用DAPI复染细胞核,但是目前哈尔滨的共聚焦都没有紫外激发光,不能激发DAPI,我怎么才能实现,用红色涂一条染色体,用绿色涂另一条,用DAPI蓝色染核,同时成像呢,所说的双光子显微镜可以么?我实在很迷惑,求求版主别再删我的帖子,尽管我现在只是一个索取者,还不能给大家提供有用的信息,但是相信有一天会为大家做贡献的,谢谢了

