Details
StuISource
Streptomycestubercidicus
ReagentsSupplied
CMXBuffer2
UnitDefinition
Oneunitistheamountofenzymerequiredtocompletelydigest1µgofumethylatedlamBDaDNAin1hourat37°Cinatotalreactionvolumeof50µl
StarActivity
No
ReactionTemperature
37°C
HeatInactivation
10minutesat65°C
AssayUnitSubstrate
LambdaDNA
ReactionBuffer
CMXBuffer2:
10mMTris-HCl(pH7.5at37°C)
10mMMgCl2
50mMNaCl
1mMdithiothreitol
100µg/mlbovineserumalbumin
ActivityinBuffers
Buffer1: 75%
Buffer2: 100%
Buffer3:50%
Buffer4:75%
Buffer5:75%
StorageBuffer
10mMTris‑HCl(pH7.4at4°C)
50mMKCl
0.1mMEDTA
1mMdithiothreitol
0.5mg/mlbovineserumalbumin
0.1mMPMSF
50%(v/v)glycerol
AssayConditions
10mMTris-HCl(pH7.5at37°C)
50mMNaCl
10mMMgCl2
1mMdithiothreitol
100µg/mlbovineserumalbumin
1µgunmethylatedlambdaDNA
Incubationisat37°Cfor1hourinatotalreactionvolumeof50µl
StorageConditions
Storeat-20°C
Shippedondryice
Downloads
CertificateofAnalysis PDF-CurrentLot
MSDSPDF -CurrentLot
Categories
RestrictionEndonucleases
ebiomall.com
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希望同路人多多交流啊!
我想用共聚焦观察间期染色体,用涂染探针,有一个问题很困扰我,我想涂染完染色后用DAPI复染细胞核,但是目前哈尔滨的共聚焦都没有紫外激发光,不能激发DAPI,我怎么才能实现,用红色涂一条染色体,用绿色涂另一条,用DAPI蓝色染核,同时成像呢,所说的双光子显微镜可以么?我实在很迷惑,求求版主别再删我的帖子,尽管我现在只是一个索取者,还不能给大家提供有用的信息,但是相信有一天会为大家做贡献的,谢谢了

