Details
TaqIISource
PurifiedfromE.colistrainthatcarriestheclonedtaqRIIgenefromThermusaquaticus
UnitDefinition
Oneunitistheamountofenzymerequiredtodigest1µgofpBR322DNAtoobtainstabledigestionpatternin1hourinatotalreactionvolumeof20µl
StarActivity
N/A
ReactionTemperature
65°C
HeatInactivation
No
AssayUnitSubstrate
pBR322
ReactionBuffer
50mMTris-HCl(pH8.5at25°C)
10mMMgCl2
1mMdithiothreitol
Notes:
- Avoidmultiplecyclesoffreezing/thawingofthestockreactionbuffernomorethan3times.
- Thawingshouldbeperformedattemperaturesnotexceeding10°C.
- Recommendedprocedureistodividetheprovidedreactionbufferintosmallerportionsandpreservethemat-70°Cforlong-term.
- Temperatureof-20°Cshouldbeusedonlyforshort-termstorage.
StorageBuffer
20mMTris-HCl(pH7.5at25°C)
0.1mMEDTA
200mMNaCl
1mMdithiothreitol
200µg/mlbovineserumalbumin
0.02%TritonX-100™
0.02%Tween20™
50%(v/v)glycerol
AssayConditions
50mMTris-HCl(pH8.5at25°C)
10mMMgCl2
1mMdithiothreitol
1 µgpBR322
TaqIIStorageConditions
Storeat-20°C
Shippedondryice
Downloads
CertificateofAnalysisPDF-CurrentLot
MSDSPDF-CurrentLot
Categories
RestrictionEndonuleases
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
希望同路人多多交流啊!
我想用共聚焦观察间期染色体,用涂染探针,有一个问题很困扰我,我想涂染完染色后用DAPI复染细胞核,但是目前哈尔滨的共聚焦都没有紫外激发光,不能激发DAPI,我怎么才能实现,用红色涂一条染色体,用绿色涂另一条,用DAPI蓝色染核,同时成像呢,所说的双光子显微镜可以么?我实在很迷惑,求求版主别再删我的帖子,尽管我现在只是一个索取者,还不能给大家提供有用的信息,但是相信有一天会为大家做贡献的,谢谢了

