![AAT Bioquest/6-TET, SE [6-Carboxy-2',4,7',7-tetrachlorofluorescein, succinimidyl ester]/211/5 mg](images/AAT201712/chemical-structure-for-6-tet-se-6-carboxy-2-4-7-7-tetrachlorofluorescein-succinimidyl-ester.png)
Overview | ![]() PrinterFriendlyVersion |
Ex/Em(nm) | 521/536 |
MW | 611.17 |
CAS# | N/A |
Solvent | DMSO |
Storage | F/D/L |
Category | ClassicLabelingDyes |
Related | DyeLabelingReagents BiochemicalAssays |
Spectrum | AdvancedSpectrumViewer |
References&Citations | ![]() PrinterFriendlyVersion |
1. Andrieu-SolerC,DoatM,HalhalM,KellerN,JonetL,BenEzraD,Behar-CohenF.(2006)Enhancedoligonucleotidedeliverytomouseretinalcellsusingiontophoresis.MolVis,12,1098.
2. PesquetE,BarbierO,RanochaP,JauneauA,GoffnerD.(2004)MultiplegenedetectionbyinsituRT-PCRinisolatedplantcellsandtissues.PlantJ,39,947.
3. TempletonKE,ScheltingaSA,SillekensP,CrielaardJW,vanDamAP,GoossensH,ClaasEC.(2003)Developmentandclinicalevaluationofaninternallycontrolled,single-tubemultiplexreal-timePCRassayfordetectionofLegionellapneumophilaandotherLegionellaspecies.JClinMicrobiol,41,4016.
4. IwamotoT,SonobeT,HayashiK.(2002)Novelalgorithmidentifiesspeciesinapolymycobacterialsamplebyfluorescencecapillaryelectrophoresis-basedsingle-strandconformationpolymorphismanalysis.JClinMicrobiol,40,4705.
5. FountainKJ,GilarM,BudmanY,GeblerJC.(2003)Purificationofdye-labeledoligonucleotidesbyion-pairreversed-phasehigh-performanceliquidchromatography.JChromatogrBAnalytTechnolBiomedLifeSci,783,61.
6. SimeonovA,NikiforovTT.(2002)Singlenucleotidepolymorphismgenotypingusingshort,fluorescentlylabeledlockednucleicacid(LNA)probesandfluorescencepolarizationdetection.NucleicAcidsRes,30,e91.
7. ReidSL,ParryD,LiuHH,ConnollyBA.(2001)BindingandrecognitionofGATATCtargetsequencesbytheEcoRVrestrictionendonuclease:astudyusingfluorescentoligonucleotidesandfluorescencepolarization.Biochemistry,40,2484.
8. PorterDJ,PreugschatF.(2000)Strand-separatingactivityofhepatitisCvirushelicaseintheabsenceofATP.Biochemistry,39,5166.
9. RyncarzAJ,GoddardJ,WaldA,HuangML,RoizmanB,CoreyL.(1999)Developmentofahigh-throughputquantitativeassayfordetectingherpessimplexvirusDNAinclinicalsamples.JClinMicrobiol,37,1941.
10. PowellLM,ConnollyBA,DrydenDT.(1998)TheDNAbindingcharacteristicsofthetrimericEcoKImethyltransferaseanditspartiallyassembleddimericformdeterminedbyfluorescencepolarisationandDNAfootprinting.JMolBiol,283,947.
AAT Bioquest AAT Bioquest是一家位于美国的生物公司,前身为ABD Bioquest,总部位于加利福尼亚州。专门从事光学检测技术十多年,一直致力于光谱学检测领域技术的创新和突破。其独特的光学检测技术,综合了化学、生物学和信息学等各个领域的研究,引领了比色、荧光和发光技术新一代光学探针的浪潮。AAT Bioquest在全球拥有强大的经验丰富的专业分销商网络,为从小型研究机构到《财富》500强企业的各类客户提供卓越的产品和定制服务。
美国AATBioquestInc.(前身是ABDBioquest,Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域,包括吸收(颜色),荧光和发光技术。AATBioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学,免疫学,细胞生物学和分子生物学等领域。AATBioquest会不断介绍新产品,快速的丰富各个领域的产品。
1)我们提供反应荧光探针和发光探针,生物素和端粒酶能够应用于标记药物小分子和生物聚合物,如蛋白、核酸以及其他碳水化合物;2)我们研究并生产荧光和发光探针用于检测蛋白,核酸和活细胞。3)我们不断的推出新型的荧光和发光探针用于检测多种酶,特别是检测水解酶和氧化还原酶类;4)我们致力于开发用于信号转导研究的试剂;5)我们提供生理和神经探针,特别是钙离子指示剂和膜电位探针。
作为AATBioquestInc.的中国区域代理,艾美捷科技为中国客户提供光谱学检测领域,包括吸收(颜色),荧光和发光技术等全系列解决方案。我们也将一如既往更加努力为国内用户提供快捷、方便的高质量产品,同时更为您售前售后全面技术支持。
AATBioquest,Inc.(formerlyABDBioquest,Inc.)develops,manufacturesandmarketsbioanalyticalresearchreagentsandkitstoscientistsengagedinlifesciencesresearch,diagnosticR&Danddrugdiscovery.Wespecializeintheareaofphotometricdetectionsincludingabsorption(color),fluorescenceandluminescencetechnologies.TheCompany"sproductsenablescientistsandbiomedicalresearcherstobetterunderstandbiochemistry,immunology,cellBIOLOGyandmolecularbiology.AATBioquestconstantlyintroducesnewproducts,andoffersarapidlyexpandinglistofproductsthataregroupedintoseveralproductlines.
1)Ourreactivefluorescentandluminescentprobes,biotinsandtagenzymesareusedforlabelingsmalldrugmoleculesandbiopolymers,e.g,proteins,nucleicacidsandcarbohydrates;2)Wedevelopfluorescentandluminescentprobesfordetectingproteins,nucleicacidsandlivecells;3)Weconstantlyintroducenovelfluorescentandluminescentprobesfordetectingvariousenzymes,inparticular,hydrolyticandredoxenzymes;4)Wefocusondevelopingreagentsforsignaltransductionresearch;and5)Wealsoofferphysiologicalandneurologicalprobes,e.g.,calciumindicatorsandmembranepotentialprobes.
Besidesthestandardcatalogproductswealsooffercustomservicetomeetyourspecialresearchneeds.Ourcurrentservicesincludecustomsynthesisofcolorimetric,fluorescentandluminescentprobes,customdevelopmentofbiochemical,cell-basedanddiagnosticassaysandcustomscreeningofyourcompoundlibrariesagainstyourdefinedtargetsusingourvalidatedHTSassays.
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然后是碱性荧光染料,造纸厂用来增加纸的亮度。
直接和分散的荧光黄,印染厂用来增加棉质和化纤面料的艳度
For principle,look at this site:
碘化丙啶(propiolium iodide,PI)能嵌入DNA双螺旋中,可使荧光强度增加约20倍,以488nm波长激发,DNA/PI复合物最大的发射波长约为615nm.
1.小鼠Lewis肺癌细胞DNA含量测定方法
(1).从C57BL/6小鼠上切除肿块,在培养皿内用PBS冲洗.
(2).去除结缔组织及脂肪,剪碎肿块.
(3).小碎片移入1.20×38mm注射针,加压使其通过,于4℃条件下重悬细胞于HBSS中.
(4).将200~300μL细胞悬液(5×105细胞/mL)中加入3mL PI(50μg/mL),染色3LL细胞,于4℃存放20~30分钟.
(5).测定580~750nm之间的发射荧光,以去除末结合PI产生的激发光与发射光谱线之间的重叠部分.
是欢快活泼的光辉色彩,是暖色系中最温暖的色,它使人联想到金色的秋天,丰硕的果实;
是一种富足、快乐而幸福的颜色。让人感觉成一种稳重、含蓄又明快的温暖;
橙色也会带来一种甜甜的感觉。

