| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 565/779 |
| MW | N/A |
| CAS# | N/A |
| Solvent | Water |
| Storage | R/D/L |
| Category | Protein-BasedTags Phycoproteins |
| Related | ProteinDyes |
| Spectrum | AdvancedSpectrumViewer |
- Pre-activateAntibodywithBuccutite™MTA
- ReconstituteBuccutite™MTAinDMSOat~10mg/mL.
Note:PleasestoreunusedMTAat-20°Candcouldbeuseduptotwofreezeandthawcycles. - Preparetargetantibody(Ab)inpH=8.5~9.0bufferatconcentrationabove1mg/ml
- AddtheMTAtoAbsolutionattheratioof8~10µgMTA/100µgAb.
- MixwellandreactatRTfor60minutes,rotatingduringthereaction.
- PurifythereactionmixturewithdesaltingcolumntoremoveunreactedMTAandexchangebuffertoPBSorbufferofyourchoice.
- CollecttheMTA-activatedAb,andestimatetheconcentrationby70%yieldoftheoriginalstartingamount.
- ReconstituteBuccutite™MTAinDMSOat~10mg/mL.
- ConjugatewithPre-activatedPE,PE-iFluors,orPE-iFluorTandems
- Reconstitutepre-activatedPE,PE,PE-iFluors,orPE-iFluorTandemsin100µLddH2Oto10mg/mL.
Note:Reconstitutedpre-activatedPE,PE-iFluors,orPE-iFluorTandemsarenotstableandcouldbestoredat4°Cforonemonth,pleasekeptitfromlight. - Addpre-activatedPE,PE-iFluors,orPE-iFluorTandemsdirectlytoMTA-activatedtargetAbsolution(from1.5)attheratioof300µgPE-iFluor647/100µgMTA-activatedAb.
- Rotatethemixturefor1~2hoursatroomtemperature
- TheAb/PE,PE-iFluors,orPE-iFluorTandemsconjugatesarenowreadytouse.
Optional:Ab/PE,PE-iFluors,orPE-iFluorTandemsconjugatecouldbefurtherpurifiedthroughsizeexclusionchromatographytogetbestperformance.
Note1:Theantibodyconjugateshouldbestoredat>0.5mg/mLinthepresenceofacarrierprotein(e.g.,0.1%bovineserumalbumin)and0.02-0.05%sodiumazide.
Note2:TheAb/PE,PE-iFluors,orPE-iFluorTandemsconjugatesolutioncouldbestoredat4°Cfortwomonthsandkeptfromlight.
- Reconstitutepre-activatedPE,PE,PE-iFluors,orPE-iFluorTandemsin100µLddH2Oto10mg/mL.
| References&Citations |  PrinterFriendlyVersion |
1. ZhaoKH,SuP,LiJ,TuJM,ZhouM,BubenzerC,ScheerH.(2006)Chromophoreattachmenttophycobiliproteinbeta-subunits:phycocyanobilin:cysteine-beta84phycobiliproteinlyaseactivityofCpeS-likeproteinfromAnabaenaSp.PCC7120.JBiolChem,281,8573.
2. PetrasekZ,SchmittFJ,TheissC,HuyerJ,ChenM,LarkumA,EichlerHJ,KemnitzK,EckertHJ.(2005)ExcitationenergytransferfromphycobiliproteintochlorophylldinintactcellsofAcaryochlorismarinastudiedbytime-andwavelength-resolvedfluorescencespectroscopy.PhotochemPhotobiolSci,4,1016.
3. LoosD,CotletM,DeSchryverF,HabuchiS,HofkensJ.(2004)Single-moleculespectroscopyselectivelyprobesdonorandacceptorchromophoresinthephycobiliproteinallophycocyanin.BiophysJ,87,2598.
4. PrasannaR,PrasannaBM,MohammadiSA,SinghPK.(2003)EvaluationofTolypothrixgermplasmforphycobiliproteincontent.FoliaMicrobiol(Praha),48,59.
5. PrasannaR,DharDW,DominicTK,TiwariON,SinghPK.(2003)IsolationandcharacterisationofphycobiliproteinrichmutantofcyanobacteriumSynechocystissp.ActaBiolHung,54,113.
6. WuP.(2000)[Phycobiliproteinandfluorescenceimmunologicalassay].ShengLiKeXueJinZhan,31,82.
7. NoubirS,LuqueI,OchoadeAldaJA,PerewoskaI,TandeaudeMarsacN,CobleyJG,HoumardJ.(2002)Co-ordinatedexpressionofphycobiliproteinoperonsinthechromaticallyadaptingcyanobacteriumCalothrixPCC7601:aroleforRcaDandRcaG.MolMicrobiol,43,749.
8. TingCS,RocapG,KingJ,ChisholmSW.(2001)PhycobiliproteingenesofthemarinephotosyntheticprokaryoteProchlorococcus:evidenceforrapidevolutionofgeneticheterogeneity.MicroBIOLOGy,147,3171.
9. TriantafilouK,TriantafilouM,WilsonKM.(2000)Phycobiliprotein-Fabconjugatesasprobesforsingleparticlefluorescenceimaging.Cytometry,41,226.
10. ZhaoKH,DengMG,ZhengM,ZhouM,ParbelA,StorfM,MeyerM,StrohmannB,ScheerH.(2000)Novelactivityofaphycobiliproteinlyase:boththeattachmentofphycocyanobilinandtheisomerizationtophycoviolobilinarecatalyzedbytheproteinsPecEandPecFencodedbythephycoerythrocyaninoperon.FEBSLett,469,9.
AAT Bioquest AAT Bioquest是一家位于美国的生物公司，前身为ABD Bioquest，总部位于加利福尼亚州。专门从事光学检测技术十多年，一直致力于光谱学检测领域技术的创新和突破。其独特的光学检测技术，综合了化学、生物学和信息学等各个领域的研究，引领了比色、荧光和发光技术新一代光学探针的浪潮。AAT Bioquest在全球拥有强大的经验丰富的专业分销商网络，为从小型研究机构到《财富》500强企业的各类客户提供卓越的产品和定制服务。
美国AATBioquestInc.(前身是ABDBioquest，Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域，包括吸收（颜色），荧光和发光技术。AATBioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学，免疫学，细胞生物学和分子生物学等领域。AATBioquest会不断介绍新产品，快速的丰富各个领域的产品。
1）我们提供反应荧光探针和发光探针，生物素和端粒酶能够应用于标记药物小分子和生物聚合物，如蛋白、核酸以及其他碳水化合物；2）我们研究并生产荧光和发光探针用于检测蛋白，核酸和活细胞。3）我们不断的推出新型的荧光和发光探针用于检测多种酶，特别是检测水解酶和氧化还原酶类；4）我们致力于开发用于信号转导研究的试剂；5）我们提供生理和神经探针，特别是钙离子指示剂和膜电位探针。
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AATBioquest,Inc.(formerlyABDBioquest,Inc.)develops,manufacturesandmarketsbioanalyticalresearchreagentsandkitstoscientistsengagedinlifesciencesresearch,diagnosticR&Danddrugdiscovery.Wespecializeintheareaofphotometricdetectionsincludingabsorption(color),fluorescenceandluminescencetechnologies.TheCompany"sproductsenablescientistsandbiomedicalresearcherstobetterunderstandbiochemistry,immunology,cellBIOLOGyandmolecularbiology.AATBioquestconstantlyintroducesnewproducts,andoffersarapidlyexpandinglistofproductsthataregroupedintoseveralproductlines.
1)Ourreactivefluorescentandluminescentprobes,biotinsandtagenzymesareusedforlabelingsmalldrugmoleculesandbiopolymers,e.g,proteins,nucleicacidsandcarbohydrates;2)Wedevelopfluorescentandluminescentprobesfordetectingproteins,nucleicacidsandlivecells;3)Weconstantlyintroducenovelfluorescentandluminescentprobesfordetectingvariousenzymes,inparticular,hydrolyticandredoxenzymes;4)Wefocusondevelopingreagentsforsignaltransductionresearch;and5)Wealsoofferphysiologicalandneurologicalprobes,e.g.,calciumindicatorsandmembranepotentialprobes.
Besidesthestandardcatalogproductswealsooffercustomservicetomeetyourspecialresearchneeds.Ourcurrentservicesincludecustomsynthesisofcolorimetric,fluorescentandluminescentprobes,customdevelopmentofbiochemical,cell-basedanddiagnosticassaysandcustomscreeningofyourcompoundlibrariesagainstyourdefinedtargetsusingourvalidatedHTSassays.
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然后是碱性荧光染料，造纸厂用来增加纸的亮度。
直接和分散的荧光黄，印染厂用来增加棉质和化纤面料的艳度
For principle,look at this site:
碘化丙啶(propiolium iodide,PI)能嵌入DNA双螺旋中,可使荧光强度增加约20倍,以488nm波长激发,DNA/PI复合物最大的发射波长约为615nm.
1.小鼠Lewis肺癌细胞DNA含量测定方法
(1).从C57BL/6小鼠上切除肿块,在培养皿内用PBS冲洗.
(2).去除结缔组织及脂肪,剪碎肿块.
(3).小碎片移入1.20×38mm注射针,加压使其通过,于4℃条件下重悬细胞于HBSS中.
(4).将200～300μL细胞悬液(5×105细胞/mL)中加入3mL PI(50μg/mL),染色3LL细胞,于4℃存放20～30分钟.
(5).测定580～750nm之间的发射荧光,以去除末结合PI产生的激发光与发射光谱线之间的重叠部分.
是欢快活泼的光辉色彩，是暖色系中最温暖的色，它使人联想到金色的秋天，丰硕的果实；
是一种富足、快乐而幸福的颜色。让人感觉成一种稳重、含蓄又明快的温暖；
橙色也会带来一种甜甜的感觉。
