![AAT Bioquest/Beta-trifluoromethylumbelliferone [7-Hydroxy-4-trifluoromethylcoumarin] *CAS 575-03-1*/82/100 mg](images/AAT201712/chemical-structure-for-beta-trifluoromethylumbelliferone-7-hydroxy-4-trifluoromethylcoumarin-cas-575-03-1.png)
| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 385/502 |
| MW | 230.14 |
| CAS# | 575-03-1 |
| Solvent | DMSO |
| Storage | F/D/L |
| Category | MicroBIOLOGy VitalStains |
| Related | BiochemicalAssays |
| Spectrum | AdvancedSpectrumViewer |
| References&Citations |  PrinterFriendlyVersion |
1. ChangTK,CrespiCL,WaxmanDJ.(2006)DeterminationofCYP2B6componentof7-ethoxy-4-trifluoromethylcoumarinO-deethylationactivityinhumanlivermicrosomes.MethodsMolBiol,320,97.
2. SridarC,GoosenTC,KentUM,WilliamsJA,HollenbergPF.(2004)SilybininactivatescytochromesP4503A4and2C9andinhibitsmajorhepaticglucuronosyltransferases.DrugMetabDispos,32,587.
3. DeanB,ArisonB,ChangS,ThomasPE,KingC.(2004)IdentificationofUGT2B9*2andUGT2B33isolatedfromfemalerhesusmonkeyliver.ArchBiochemBiophys,426,55.
4. GhosalA,HapangamaN,YuanY,Achanfuo-YeboahJ,IannucciR,ChowdhuryS,AltonK,PatrickJE,ZbaidaS.(2004)IdentificationofhumanUDP-glucuronosyltransferaseenzyme(s)responsiblefortheglucuronidationofposaconazole(Noxafil).DrugMetabDispos,32,267.
5. EkinsS,BerbaumJ,HarrisonRK.(2003)Generationandvalidationofrapidcomputationalfiltersforcyp2d6andcyp3a4.DrugMetabDispos,31,1077.
6. JinnoH,SaekiM,Tanaka-KagawaT,HaniokaN,SaitoY,OzawaS,AndoM,ShiraoK,MinamiH,OhtsuA,YoshidaT,SaijoN,SawadaJ.(2003)Functionalcharacterizationofwild-typeandvariant(T202IandM59I)humanUDP-glucuronosyltransferase1A10.DrugMetabDispos,31,528.
7. HuynhHT,TeelRW.(2002)Effectsofplant-derivedphenolsonratlivercytochromeP4502B1activity.AnticancerRes,22,1699.
8. RenwickAB,LavignetteG,WorboyPD,WilliamsB,SurryD,LewisDF,PriceRJ,LakeBG,EvansDC.(2001)Evaluationof7-benzyloxy-4-trifluoromethylcoumarin,someother7-hydroxy-4-trifluoromethylcoumarinderivativesand7-benzyloxyquinolineasfluorescentsubstratesforrathepaticcytochromeP450enzymes.Xenobiotica,31,861.
9. RenwickAB,LewisDF,FulfordS,SurryD,WilliamsB,WorboysPD,CaiX,WangRW,PriceRJ,LakeBG,EvansDC.(2001)Metabolismof2,5-bis(trifluoromethyl)-7-benzyloxy-4-trifluoromethylcoumarinbyhumanhepaticCYPisoforms:evidenceforselectivitytowardsCYP3A4.Xenobiotica,31,187.
10. RenwickAB,SurryD,PriceRJ,LakeBG,EvansDC.(2000)Metabolismof7-benzyloxy-4-trifluoromethyl-coumarinbyhumanhepaticcytochromeP450isoforms.Xenobiotica,30,955.
AAT Bioquest AAT Bioquest是一家位于美国的生物公司，前身为ABD Bioquest，总部位于加利福尼亚州。专门从事光学检测技术十多年，一直致力于光谱学检测领域技术的创新和突破。其独特的光学检测技术，综合了化学、生物学和信息学等各个领域的研究，引领了比色、荧光和发光技术新一代光学探针的浪潮。AAT Bioquest在全球拥有强大的经验丰富的专业分销商网络，为从小型研究机构到《财富》500强企业的各类客户提供卓越的产品和定制服务。
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然后是碱性荧光染料，造纸厂用来增加纸的亮度。
直接和分散的荧光黄，印染厂用来增加棉质和化纤面料的艳度
For principle,look at this site:
碘化丙啶(propiolium iodide,PI)能嵌入DNA双螺旋中,可使荧光强度增加约20倍,以488nm波长激发,DNA/PI复合物最大的发射波长约为615nm.
1.小鼠Lewis肺癌细胞DNA含量测定方法
(1).从C57BL/6小鼠上切除肿块,在培养皿内用PBS冲洗.
(2).去除结缔组织及脂肪,剪碎肿块.
(3).小碎片移入1.20×38mm注射针,加压使其通过,于4℃条件下重悬细胞于HBSS中.
(4).将200～300μL细胞悬液(5×105细胞/mL)中加入3mL PI(50μg/mL),染色3LL细胞,于4℃存放20～30分钟.
(5).测定580～750nm之间的发射荧光,以去除末结合PI产生的激发光与发射光谱线之间的重叠部分.
是欢快活泼的光辉色彩，是暖色系中最温暖的色，它使人联想到金色的秋天，丰硕的果实；
是一种富足、快乐而幸福的颜色。让人感觉成一种稳重、含蓄又明快的温暖；
橙色也会带来一种甜甜的感觉。
