Recombinant Human His6-PolyUb WT Chains (2-7,K63-linked), CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
UCH-330
| Formulation | X mg/ml in sterile, deionized water |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Poly-Ubiquitin
Poly-Ubiquitin chains are composed of Ubiquitin monomers that are covalently linked through isopeptide bonds, which typically form between a lysine residue of one Ubiquitin molecule and the C-terminal glycine residue of another Ubiquitin molecule (1). Each human Ubiquitin monomer is 76 amino acids (aa) in length and shares 96% and 100% aa identity with yeast and mouse Ubiquitin, respectively (2). Seven of the 76 aa in Ubiquitin are lysine residues that can participate in poly-Ubiquitin chain formation. Linkage through specific lysine residues is thought to serve as a signal that affects protein degradation, signaling, trafficking, and other cellular processes (3-8).
Linkage specific poly-Ubiquitin chains are used to investigate mechanisms of chain recognition, binding and hydrolysis by the proteasome, deubiquitinating enzymes, E3 ligases or other proteins that contain Ubiquitin-associated domains (UBAs) or ubiquitin-interacting motifs (UIMs). Lys63-linked poly-Ubiquitin has been implicated in several non-degradative processes such as receptor endocytosis and sorting, translation, DNA damage repair, the stress response and signaling in theNF kappa B pathway. This product is formed with wild-type human recombinant Ubiquitin and linkage-specific enzymes. This mixture of poly-Ubiquitin chains contains di-Ubiquitin and higher MW species; mono-Ubiquitin has been removed.
The His6-tag is convenient for metal chelate affinity purification and immuno-detection using His6-specific antibodies.
- Scheffner, M. et al. (1995) Nature 373:81.
- Sharp, P.M. & W.-H. Li (1987) Trends Ecol. Evol. 2:328.
- Behrends, C. & J.W. Harper (2011) Nat. Struct. Mol. Biol. 18:520.
- Greene, W. et al. (2012) PLoS Pathog. 8:e1002703.
- Henry, A.G. et al. (2012) Dev. Cell 23:519.
- Tong, X. et al. (2012) J. Biol. Chem. 287:25280.
- Wei, W. et al. (2004) Nature 428:194.
- Zhang, J. et al. (2012) J. Biol. Chem. 287:28646.
Citation for Recombinant Human His6-PolyUb WT Chains (2-7,K63-linked), CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
1Citation: Showing 1 - 1
- Cyclophilin J limits inflammation through the blockage of ubiquitin chain sensingAuthors: C Sheng, C Yao, Z Wang, H Chen, Y Zhao, D Xu, H Huang, W Huang, S ChenNat Commun, 2018;9(1):4381.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
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3-硝基邻苯二甲酸可作为鲁米诺的合成原料。3-硝基邻苯二甲酸与肼在高沸点溶剂（如二甘醇）中发生缩合反应，失去一分子水，生成3-硝基邻苯二甲酰肼。然后以保险粉还原3-硝基邻苯二甲酰肼中的硝基，得到3-氨基邻苯二甲酰肼，即是鲁米诺。
我们常说的鲁米诺试剂是鲁米诺与过氧化氢（双氧水的主要成分）的混合物，主要用于现代刑侦的的血液检测。鲁米诺与氢氧化物反应时生成了一个双负离子（Dianion），它可被过氧化氢分解出的氧气氧化，产物为一个有机过氧化物。该过氧化物很不稳定，立即分解出氮气（鲁米诺被有机氧化剂如二甲基亚砜氧化后不是生成氮气，而是生成含氮有机物），生成激发态的3-氨基邻苯二甲酸。激发态至基态转化中，释放的能量以光子的形式存在，波长位于可见光的蓝光部分。鲁米诺只有用氧化剂处理过才会发光。通常使用双氧水和一种氢氧化物碱的混合水溶液作为激发剂。在铁化合物催化下，双氧水分解为氧气和水：2H2O2→O2+2H2O实验室中常以铁氰化钾作为催化剂铁的来源，而法医学上的催化剂则恰好是血红蛋白中的铁。很多生物系统中的酶也可催化过氧化氢的分解反应。鲁米诺试剂使用识别血液的试剂，血迹即使被擦拭，血液中的血红素还是会残留下来，当鲁米诺试剂喷在血红素上，会与活性氧产生氧化作用，释放出蓝紫色荧光。被成为鲁米诺反应。是鉴定血液用的有机物质。
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有荧光为何还需要压片这么久呢？不知各位用的时候有无这种情况！
