
Product Name | SensoLyte ® AFC Caspase Substrate Sampler Kit *Fluorimetric* |
Size | 1 kit |
Catalog # | AS-71117 |
US$ | $563 |
Caspases play important roles in apoptosis and cell signaling. They are also identified as drug-screening targets. AFC-based substrates yield blue fluorescence upon protease cleavage (Ex/Em=380 nm/500 nm). They are widely used to monitor caspase activity. The SensoLyte® Caspase Substrate Kit contains a series of AFC-based peptide substrates as fluorogenic indicators for assaying caspase protease activities. It provides the best solution for identifying a suitable substrate for designing AFC-based caspase assays. All the components in the kit are available in bulk package. The kit contains: 8 AFC caspase substrates Assay buffer AMC (fluorescence reference standard for calibration) A detailed protocol Kit Size: 8 x 100 assays | |
Detailed Information | ![]() ![]() |
Storage | -20°C |
Product Citations | Twiner, MJ. et al. (2012). Induction of apoptosis pathways in several cell lines following exposure to the marine algal toxin Azaspiracid. Chem Res Toxicol 25, 1493.Gugliesi, F. et al. (2010). The proapoptotic activity of the Interferon-inducible gene IFI16 provides new insights into its etiopathogenetic role in autoimmunity. J Autoimmun doi:10.1016/j.jaut.2010.04.001. |
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如常用于缓冲的Tris、HEPES、MPOS等等,去垢剂如NP40,Tween-20,TritonX100,CHAPS,脱氧胆酸钠,SDS等等,
大家能说说这些试剂的使用区别吗(最好讲讲某些情况下使用一些会优于另一些的例子)
有时候某些裂解液含10%左右甘油,又是为何?
平时我们用习惯了一些配方,可能有时候不太清楚为何有些是可替代的,有些是不可替代的,借此机会想听听大家的见解?
去垢剂作为添加剂生长蛋白质晶体是一项很有用的技术。非离子和两性离子结晶剂在膜蛋白结晶中已经得到很好应用,目前已经变成常规的实验手段。去垢剂在一些可溶蛋白中也起到了一定的作用,提高了晶体的质量和结果的可重复性 ,促进了单晶的生长, 但具体的影响机制尚不明确。其可能原因是去垢剂的引入使结晶液滴中的蛋白分子更具有亲水性,使溶液中同质蛋白质分子增加。向左转|向右转
如要膜蛋白保持原来的结构应采用:非离子型去垢剂.

