
Properties:
Related Categories | Aluminum TLC Plates: Silica matrix, Aluminum TLC Plates: fluorescent indicator, Analytical/Chromatography, LPLC/TLC/Paper Chromatography, TLC Plates, Aluminum foil backing, |
material | aluminum support |
silica gel matrix | |
quality | with fluorescent indicator 254 nm |
feature | binder Polymeric |
fluorescent indicator | |
mfr. no. | Sigma-Aldrich |
application(s) | thin layer chromatography (TLC): suitable |
Description:
Application
Silica gel on TLC Alu foils may be used to perform thin layer chromatography.
General description
Silica gel is a rigid 3D network of colloidal silica. It is exclusively used as a support material for the active titanium(III) centers in Ziegler-Natta catalysts. Silica gel allows a controlled fragmentation resulting in the formation of uniform polymer particles with narrow particle size distribution and high bulk density. Silica gel is widely used as layer material for TLC. Plaster of Paris is generally used as binding agent to hold silica gel firmly to the TLC plates. Silica gel is applied as sorbent to the aluminium foils.
Safety:
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如常用于缓冲的Tris、HEPES、MPOS等等,去垢剂如NP40,Tween-20,TritonX100,CHAPS,脱氧胆酸钠,SDS等等,
大家能说说这些试剂的使用区别吗(最好讲讲某些情况下使用一些会优于另一些的例子)
有时候某些裂解液含10%左右甘油,又是为何?
平时我们用习惯了一些配方,可能有时候不太清楚为何有些是可替代的,有些是不可替代的,借此机会想听听大家的见解?
去垢剂作为添加剂生长蛋白质晶体是一项很有用的技术。非离子和两性离子结晶剂在膜蛋白结晶中已经得到很好应用,目前已经变成常规的实验手段。去垢剂在一些可溶蛋白中也起到了一定的作用,提高了晶体的质量和结果的可重复性 ,促进了单晶的生长, 但具体的影响机制尚不明确。其可能原因是去垢剂的引入使结晶液滴中的蛋白分子更具有亲水性,使溶液中同质蛋白质分子增加。向左转|向右转
如要膜蛋白保持原来的结构应采用:非离子型去垢剂.


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