BackgroundThe calcium channel alpha2/delta subunit is a glycosylated structural subunit consisting of the alpha2 subunit and the delta peptide. There isdistinctive alpha2 subunit expression in rat spinal cord and dorsal root ganglia (DRG)There are two forms of the alpha2 subunit in DRG that are different from the alpha2 subunit in other tissues examined, at least at the glycosylation level. Thus, post-translational modification may be important in tissue specific and functional expression of the alpha2/delta subunit (2).alpha2/delta and beta1b interact with alpha1G to increase trafficking of, or stabilize, functional alpha1G channels expressed at the plasma membrane.(3)

Source

Immunogen: Synthetic peptide derived from the rat alpha2/delta calcium channel conjugated to KLH

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Ammonium Sulfate Precipitation

Concentration: See vial for concentration

ApplicationsThis antibody can be used for Western blotting(5-10 µg/ml).Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Rat brain lysate

StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Stability: Products are stable for one year from purchase when stored properly

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.

References1. Kim, H.L et.al. "Rat brain expresses an alternatively spliced form of the dihydropyridine-sensitive L-type calcium channel alpha 2 subunit." Proc. Natl. Acad. Sci. U.S.A. 89 (8), 3251-3255 (1992)2. Luo, Z.D. "Rat dorsal root ganglia express distinctive forms of the alpha2 calcium channel subunit." Neuroreport.;11(16):3449-52 (2000)3. Dolphin, AC et al. "The effect of alpha2-delta and other accessory subunits on expression and properties of the calcium channel alpha1G." J Physiol.;519 Pt 1:35-45.(1999)

Protein Reference(s)

Database Name: UniProt

Accession number: Q8VHS9

Species Accession: Rat

Safety Datasheet(s) for this product:EA_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium AzideV2.pdf