BackgroundThe Kv1.4 potassium channel is a voltage-gated channel protein which belongs to the delayed rectifier class and to the Shaker potassium channel subfamily which includes Kv1.1, Kv1.2, Kv1.3 and Kv1.5.Potassium channels are mainly found in plasma membranes but are not generally distributed over the cell surface. Potassium channels catalyze the rapid permeation of potassium ions while rejecting biologically abundant potential competitors such as sodium, calcium and magnesium.Ion selectivity and high through put rate of potassium channels is accomplished by precise co-ordination of dehydrated potassiumby the protein and multiple ion occupancy within the permeation pathway.All potassium channels carry out the formation of a transmembrane leak specific for potassium ions.Since cells almost universally maintain cytoplasmic potassium concentrations higher than those extracellularly, the opening of a potassium channel implies a negative ongoing change in electrical voltage across the cell membrane. This may result in termination of the action potential of electrically excitable cells including nerve, muscle and pancreatic beta cells. In nonÐexcitable cells, potassium channels play important roles in the cellular potassium recycling required for electrolyte balance effected by the renal epithelium.

Synonyms: Voltage-Gated Potassium Channel, Kv1.4 isoform

Source

Immunogen: Synthetic peptide derived from the N-terminus of the rat Kv1.4 potassium channel conjugated to KLH

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Ammonium Sulfate Precipitation

Concentration: See vial for concentration

ApplicationsThis antibody can be used for Western blotting (5-10 µg/ml).Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Stability: Products are stable for one year from purchase when stored properly

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.

References1. Matsubara, H., et al. "Shaker-related potassium channel, Kv1.4, mRNA regulation in cultured rat heart monocytes and differential expression of Kv1.4 and Kv1.5 genes in myocardial development and hypertrophy." J. Clin. Invest. 1993, 92 1659-16662. Rasmusson, R.L., et al. "C-type inactivation control recovery in a fast inactivating cardiac K+ channel (Kv1.4) expressed in Xenopus oocytes." J. Physiol. 1995, 489, 709-7213. Guo, W., et al. "Regulation of Kv4.2 and Kv1.4 K+ channel expression by myocardial hypertrophic factors in cultured newborn rat ventricular cells." J. Mol. Cell. Cardiol. 1998, 30, 1449-14554. Chanda, B., et al. "Transplanting the N-terminus from Kv1.4 to Kv1.1 generates an inwardly rectifying K+ channel." Neuroreport 1999, 10, 237-241

Protein Reference(s)

Database Name: SwissProt

Accession number: P22459

Species Accession: Human

Safety Datasheet(s) for this product:EA_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium AzideV2.pdf