| Isotype | IgG |
| Product Type | Polyclonal Antibody |
| Units | 100 µg |
| Host | Rabbit |
| Species reactivity | Rat |
| Application | ImmunofluorescenceImmunoprecipitationWestern BlotWestern Blotting |
BackgroundPore-forming (alpha) subunit of voltage-gated rapidly inactivating A-type potassium channels. May contribute to Icurrent in heart and current in neurons. Channel properties are modulated by interactions with other alpha subunits and with regulatory subunits In response to membrane depolarization, Kv4.2 channels accumulate in the closed-inactivated state(s), from which they directly recover, bypassing the open state.Kv4.2 is a substrate for ERK in vitro and in vivo, and suggest that ERK may regulate potassium-channel function by direct phosphorylation of the pore-forming alpha subunit.
Synonyms: RK5, Shal1, Kcnd2, Potassium voltage-gated channel subfamily D member 2
Source
Immunogen: Peptide made to a.a. 453-470 of rat Kv4.2 conjugated to KLH
Product
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Ammonium Sulfate Precipitation
Concentration: See vial for concentration
ApplicationsOptimal concentration should be evaluated by serial dilutions. Suggested Western Blot dilution 1:1000.Other applications:Immunoprecipitation, immunofluorescence.
Functional Analysis: Western Blotting
Positive Control: Highly expressed in heart and throughout the brain, with similar levels in cortex and hypothalamus, and much higher levels in hippocampus, dentate gyrus and the habenular nucleus of the thalamus. Detected at similar levels in heart atrium and ventricle. Detected in aorta, cardiac and smooth muscle.
StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Product Stability: Products are stable for one year from purchase when stored properly
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.
References1. Roberds,S.L. et.al, Cloning and tissue-specific expression of five voltage-gated potassium channel cDNAs expressed in rat heart. Proc. Natl. Acad. Sci. U.S.A. 88 (5), 1798-1802 (1991)2. Bahring, K.et.al. Kinetic analysis of open- and closed-state inactivation transitions in human Kv4.2 A-type potassium channels. J Physiol ;535(Pt 1):65-8120013. Adams, T. et.al,The A-type potassium channel Kv4.2 is a substrate for the mitogen-activated protein kinase ERK. J Neurochem 5(6):2277-87 2000
Protein Reference(s)
Database Name: UniProt
Accession number: Q63881
Species Accession: Rat
Safety Datasheet(s) for this product:EA_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium AzideV2.pdf
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说得简单点就是，细胞核是原封不动的，没有重组过，而细胞核外的东西是很多别的细胞中提取出来的并组合起来的。这样的混合体淋巴细胞产生的抗体具有很强的嵌合能力，针对性比一般的抗体强，但是它的来源没变，所以称之为混合单克隆抗体
制备抗体都需要进行纯化处理
清冷冻干燥后保存。
没有marker，怎么知道你做的蛋白大小？
没有参照物，怎么知道你跑的快不快？
没有尺子，怎么知道你的size大小？
凭嘴说吗？
单克隆抗体这项新技术从根本上解决了在抗体制备中长期存在的特异性和可重复性问题，可用于探讨: ①蛋白质的精细结构；②淋巴细胞亚群的表面新抗原；③组织相容性抗原；④激素和药物的放射免疫（或酶免疫）分析；⑤肿瘤的定位和分类；⑥纯化微生物和寄生虫抗原；⑦免疫治疗和与药物结合的免疫-化学疗法 (“导弹”疗法,利用单克隆抗体与靶细胞特异性结合，将药物带至病灶部位.。
因此，单克隆抗体可直接用于人类疾病的诊断、预防、治疗以及免疫机制的研究，为人类恶性肿瘤的免疫诊断与免疫治疗开辟了广阔前景。
