BackgroundLeukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFand bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NF B dependent signalling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations

Synonyms: CD62 antigen-line family member E; Endothelial leukocyte adhesion molecule 1

Source

Immunogen: Hybridoma produced by the fusion of splenocytes from Lewis rat immunized with LPS-activated mlEND1 cells expressing E-selectin at their cell surface and mouse Sp2/0 myeloma cells.

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Protein A/G Chromatography

Concentration: See vial for concentration

ApplicationsAntibody suitable for immunoblotting, ELISA, immunoprecipitation, immunohistochemistry on frozen sections and flow cytometry (1:200 dilution).Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: This antibody recognizes a conformational epitope in the CR1 region in murine E-selectin.Also partially inhibits leukocyte adhesion to murine activated endothelial cells.

StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Stability: Products are stable for one year from purchase when stored properly

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.

References1.Hallmann, R., et al. (1995). Adhesion of leukocytes to the inflammed endothelium, Scand J Rheumatol Suppl 101, 107-9.2.Hammel, M., et al. (2001). Species-specific and conserved epitopes on mouse and human E-selectin important for leukocyte adhesion, Exp Cell Res 269, 266-74.

Protein Reference(s)

Database Name: UniProt

Accession number: Q00690

Species Accession: Human

Safety Datasheet(s) for this product:EA_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium AzideV2.pdf