| Isotype | IgG |
| Product Type | Blocking Peptide |
| Units | 50 µg |
| Species reactivity | Human |
| Application | Antibody function blockingFunctional Inhibition |
BackgroundSphingosine 1-phosphate (S1P) is a highly bioactive lipid that has a myriad of biological effects both intracellularly as a second messenger and extracellularly by binding to the S1P(1-5)/G-protein-coupled receptors of the endothelial differentiation gene family.Intracellularly, at least two enzymes, sphingosine kinase (1 and 2) and S1P phosphatase, regulate the activity of S1P by governing the phosphorylation of S1P.Regulation of hS1Pase1 with the resultant changes in cellular and secreted S1P has important implications in cell proliferation, angiogenesis and apoptosis.
Product
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Concentration: See vial for concentration
ApplicationsFor use with Sphingosine 1-Phosphate Phosphatase 1 polyclonal antibody (Cat. No. X1636P).
StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Product Stability: Products are stable for one year from purchase when stored properly
CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.
References1. Mechtcheriakova D, et al. Sphingosine 1-phosphate phosphatase 2 is induced during inflammatory responses. Cell Signal. 2007 Apr;19(4):748-60. Epub 2006 Sep 30. Links 2. Grey, A., et al. "Osteoblastic Cells Express Phospholipid Receptors and Phosphatases and Proliferate in Response to Sphingosine 1-Phosphate."Calcid Tissue Int. 20043. Pettus, B.J., et al. "The sphingosine kinase 1/sphingosine 1-phosphate pathway mediates COX-2 induction and PGE2 production in response to TNF-alpha." FASEB J. 2003, 17, 1411-14214. Johnson, K.R., et al. "Role of human sphingosine 1-phosphate phosphatase 1 in the regulation of intra- and extracellular sphingosine 1-phosphate levels and cell viability." J. Biol. Chem. 2003, 278, 34541-345475. Cuvillier, O. "Sphingosine in apoptosis signalling." Biochim. Biophys. Acta 2002, 1585, 153-1626. Ogawa, C., et al. "Identification and characterization of a novel human sphingosine 1-phosphate phosphohydrolase, hSPP2." J. Biol. Chem. 2003, 278, 1268-12727. Le Stunff, H., et al. "Characterization of murine sphingosine 1-phosphate phosphohydrolase." J. Biol. Chem. 2002, 277, 8920-89278. Birchwood, C.J., et al. "Calcium influx and signaling in yeast stimulated by intracellular sphingosine 1-phosphate accumulation." J. Biol. Chem. 2001, 276, 11712-11718
Protein Reference(s)
Database Name: SwissProt
Accession number: Q9BX95
Species Accession: Human
Safety Datasheet(s) for this product:EA_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium AzideV2.pdf
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说得简单点就是，细胞核是原封不动的，没有重组过，而细胞核外的东西是很多别的细胞中提取出来的并组合起来的。这样的混合体淋巴细胞产生的抗体具有很强的嵌合能力，针对性比一般的抗体强，但是它的来源没变，所以称之为混合单克隆抗体
制备抗体都需要进行纯化处理
清冷冻干燥后保存。
没有marker，怎么知道你做的蛋白大小？
没有参照物，怎么知道你跑的快不快？
没有尺子，怎么知道你的size大小？
凭嘴说吗？
单克隆抗体这项新技术从根本上解决了在抗体制备中长期存在的特异性和可重复性问题，可用于探讨: ①蛋白质的精细结构；②淋巴细胞亚群的表面新抗原；③组织相容性抗原；④激素和药物的放射免疫（或酶免疫）分析；⑤肿瘤的定位和分类；⑥纯化微生物和寄生虫抗原；⑦免疫治疗和与药物结合的免疫-化学疗法 (“导弹”疗法,利用单克隆抗体与靶细胞特异性结合，将药物带至病灶部位.。
因此，单克隆抗体可直接用于人类疾病的诊断、预防、治疗以及免疫机制的研究，为人类恶性肿瘤的免疫诊断与免疫治疗开辟了广阔前景。
