ORDERINGINFORMATION
CatalogNo.:43037
Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.
BACKGROUND
Elk-1isamemberoftheEtsfamilyoftranscriptionfactorsandoftheternarycomplexfactor(TCF)subfamily.ProteinsoftheTCFsubfamilyformaternarycomplexbybindingtotheserumresponsefactorandtheserumreponseelementinthepromoterofthec-fosproto-oncogene.Theproteinencodedbythisgeneisanucleartargetfortheras-raf-MAPKsignalingcascade.
Alternativelysplicedtranscriptvariantsencodingthesameproteinhavebeenfoundforthisgene.
SPECIFICATIONSUMMARY
Antigen:Peptidesequencethatincludesphosphorylationsiteofserine389(P-R-S(p)-P-A)derivedfromhumanElk1andconjugatedtoKLH.
Accessionno.:P19419,NP_001107595.1
HostSpecies:Rabbit
Specificity:Thisantibodydetectsendogenoushuman,mouse,andratElk1onlywhenphosphorylatedatserine389.
APPLICATION
Immunoblotting:useatdilutionof1:500-1:1,000.Abandof~62kDaisdetected.
Immunohistochemistry:useatdilutionof1:50-1:100.
DetectionofElk1(phospho-Ser389)inextractsofHT29cellsuntreatedortreatedwithAnisomycin.
DetectionofElk1(phospho-Ser389)inparaffin-embeddedhumanbreastcarcinomatissue.
Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-termuse.
Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.
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比如你用anti-MHC-II，mouse IgG1-FITC，那同型就用非特异性的mouse IgG1-FITC，同样浓度
请问：同型对照应该怎么设？
不买专门的IgG2Bisotypecontrol，而用其他的抗体，
比如mouseanti-humanCD117，Igclass也是IgG2B，可以吗？
阴性对照(Isotype Control)：非特异荧光的强弱取决于抗体浓度、单克隆荧光抗体特异性和纯度，应与试验管抗体相对应。在多色分析时，同型对照应与其它抗体同时使用，以避免补偿造成的误差
血小板体外活化试验：使用正常人活化标本作为阳性质控；使用正常人未活化标本作为阴性质控
血小板自身抗体检测：使用含有已知血小板抗体的血清与血小板孵育，作为阳性质控；使用不含血小板抗体的血清与血小板孵育，作为阴性质控
血小板表面抗原缺失：如巨血小板症血小板表面CD42a/CD42b缺失，血小板无力症血小板表面gpIIb/IIIa，即CD41/CD61缺失或异常。使用正常人标本做阳性对照，抗体的同型对照做阴性对照
英 ['aɪsətaɪp]美 ['aɪsəˌtaɪp]
n. 同型动物（或植物），图形文字，象征性图像;同位型;同模;同号模式
英 ['aisəutaip]
美 ['aisəutaip]
n. 同形像；
同形像统计图
