ATXN3PolyclonalAntibody
CatalogNo.:AB10089-200
Price/Unit:EUR320.00
Source:Goat
Quantity/unitsize:600µg
Description:GoatpolyclonalantibodytoATXN3.Thisproteinappearstobeacomponentoftheubiquitinproteasomesystemandhasdeubiquitinaseactivity.Itmayalsohaverolesinneuroprotection,proteinhomeostasismaintenance,transcriptionalandCytoskeletonregulation,myogenesisanddegradationofmisfoldedchaperonesubstrates.TheproteincontainsQnrepeatsinthecodingregion,andtheexpansionoftheserepeatsfromthenormal13-36to68-79isonecauseofMachado-Josephdiseasealsoknownasspinocerebellarataxia-3,anautosomaldominantneurologicdisorder.Alternativenames:AT3,ATX3,ataxin-3,ataxin3varianth,autosomaldominantataxin3,ataxin3variantm,ataxin3variantref,JOS,josephin,Machado-Joseph,Machado-Josephdiseaseprotein1,MJD,MJD1,SCA3,spinocerebellarataxia3,olivopontocerebellarataxia3,spinocerebellarataxiatype3proteinantibody.
Form:Polyclonalantibodysuppliedasa200µl(3mg/ml)aliquotinPBS.Itdoesnotcontainpreservatives.GlycerolandazideFREE.Thisantibodyisepitope-affinitypurifiedfromgoatantiserum.
Immunogen:Purifiedrecombinantpeptidederivedfromwithinresidues120aato250aaofhumanATXN3producedinE.coli.
Specificity:DetectslevelsofATXN3proteinintransfectedcellswithGFP-ATXN3andendogenouslevelsinU118,SH-SY5Y,At-T20andhTERTcelllysatesbyWesternblot.ThisAbissimilartoAB0089.
Reactivity:Reactsagainsthuman,rat,mouse,monkeyandcanineproteins.
Usage:Westernblot 1:250-1:2,000Immunofluorescence NDImmunohistochemistry(paraffin) NDImmunohistochemistry(frozen) ND
Reactivitychart
| Sample | WesternBlot | Immuno-fluorescence | Immuno-Histochemistry(p) | Immuno-Histochemistry(f) | human | +++ | ND | ND | ND | rat | +++ | ND | ND | ND | mouse | +++ | ND | ND | ND | canine | +++ | ND | ND | ND | monkey | +++ | ND | ND | ND |
|---|
Storage:Storeat-20Cforlongtermstorage.Storeat2-8Cforuptoonemonth.
Specialinstructions:Avoidfreeze/thawcycles.

References
Forresearchuseonly,notfordiagnosticuse
ebiomall.com
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比如你用anti-MHC-II,mouse IgG1-FITC,那同型就用非特异性的mouse IgG1-FITC,同样浓度
请问:同型对照应该怎么设?
不买专门的IgG2Bisotypecontrol,而用其他的抗体,
比如mouseanti-humanCD117,Igclass也是IgG2B,可以吗?
阴性对照(Isotype Control):非特异荧光的强弱取决于抗体浓度、单克隆荧光抗体特异性和纯度,应与试验管抗体相对应。在多色分析时,同型对照应与其它抗体同时使用,以避免补偿造成的误差
血小板体外活化试验:使用正常人活化标本作为阳性质控;使用正常人未活化标本作为阴性质控
血小板自身抗体检测:使用含有已知血小板抗体的血清与血小板孵育,作为阳性质控;使用不含血小板抗体的血清与血小板孵育,作为阴性质控
血小板表面抗原缺失:如巨血小板症血小板表面CD42a/CD42b缺失,血小板无力症血小板表面gpIIb/IIIa,即CD41/CD61缺失或异常。使用正常人标本做阳性对照,抗体的同型对照做阴性对照
英 ['aɪsətaɪp]美 ['aɪsəˌtaɪp]
n. 同型动物(或植物),图形文字,象征性图像;同位型;同模;同号模式
英 ['aisəutaip]
美 ['aisəutaip]
n. 同形像;
同形像统计图

