ELISA Kit for Anti-Interferon Gamma Antibody (Anti-IFNg)
IFN-G; IFG; IFI; INFr; IFN, Immune Interferon
- Product No.AEA049Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Test MethodIndirect ELISA
- Assay Length2h, 30min
- Detection Range3.12-200ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 1.14ng/mL.
- Sample TypeSerum
- DownloadInstruction Manual
- UOM48T96T96T*596T*1096T*100
- FOBUS$ 630 For more details, please contact local distributors!US$ 900 For more details, please contact local distributors!US$ 4050 For more details, please contact local distributors!US$ 7650 For more details, please contact local distributors!US$ 63000 For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- Results demonstration
- Typical Standard Curve
- ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the ELISA Kit for Anti-Interferon Gamma Antibody (Anti-IFNg)
This assay has high sensitivity and excellent specificity for detection of Anti-Interferon Gamma Antibody (Anti-IFNg).No significant cross-reactivity or interference between Anti-Interferon Gamma Antibody (Anti-IFNg) and analogues was observed.
Recovery of the ELISA Kit for Anti-Interferon Gamma Antibody (Anti-IFNg)
Matrices listed below were spiked with certain level ofAnti-Interferon Gamma Antibody (Anti-IFNg) and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-Interferon Gamma Antibody (Anti-IFNg) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 78-93 | 87 |
Precision of the ELISA Kit for Anti-Interferon Gamma Antibody (Anti-IFNg)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Interferon Gamma Antibody (Anti-IFNg) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Interferon Gamma Antibody (Anti-IFNg) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>
Linearity of the ELISA Kit for Anti-Interferon Gamma Antibody (Anti-IFNg)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-Interferon Gamma Antibody (Anti-IFNg) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 92-101% | 78-98% | 85-97% | 78-98% |
Stability of the ELISA Kit for Anti-Interferon Gamma Antibody (Anti-IFNg)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the ELISA Kit for Anti-Interferon Gamma Antibody (Anti-IFNg)
1. Prepare all reagents, samples and standards;2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;4. Aspirate and wash 5 times;5. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;6. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle of the ELISA Kit for Anti-Interferon Gamma Antibody (Anti-IFNg)
The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Interferon Gamma Antibody (Anti-IFNg) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Interferon Gamma Antibody (Anti-IFNg) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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英 ['aɪsətaɪp]美 ['aɪsəˌtaɪp]
n.
同型动物（或植物），图形文字，象征性图像;同位型;同模;同号模式
网络
同型;同种型;模式标本
双语例句
Study on the Action of Decreasing level of Isotype Cysteine for High Blood Viscosity
降低同型半胱氨酸水平对高黏血症作用的研究
比如你用anti-MHC-II，mouse IgG1-FITC，那同型就用非特异性的mouse IgG1-FITC，同样浓度
选用和特异性抗体同种亚型的，同样荧光素标记的抗体，采用同样的染色步骤染同样浓度的细胞。然后上机检测。如果细胞有非特异性的结合，那同型对照的荧光强度可能会比未染色的阴性对照要高一些。
同型对照只用于那种阳性和阴性之间没有明确分解的情况。如果阳性细胞群非常清楚，是不需要同型对照的。另外，在多色同染的情况下，也不采用同型对照，而是采用FMO（fluorensence minus one）对照。
另外，对于同型对照的使用，也有很多反对意见。就算是同型抗体，也可能会有不同的亲和力，并不一定就能真实反应非特异性的结合。
