ELISA Kit for Anti-Interferon Gamma Antibody (Anti-IFNg)
IFN-G; IFG; IFI; INFr; IFN, Immune Interferon
- Product No.AEA049Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Test MethodIndirect ELISA
- Assay Length2h, 30min
- Detection Range3.12-200ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 1.14ng/mL.
- Sample TypeSerum
- DownloadInstruction Manual
- UOM48T96T96T*596T*1096T*100
- FOBUS$ 630 For more details, please contact local distributors!US$ 900 For more details, please contact local distributors!US$ 4050 For more details, please contact local distributors!US$ 7650 For more details, please contact local distributors!US$ 63000 For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- Results demonstration
- Typical Standard Curve
- ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the ELISA Kit for Anti-Interferon Gamma Antibody (Anti-IFNg)
This assay has high sensitivity and excellent specificity for detection of Anti-Interferon Gamma Antibody (Anti-IFNg).No significant cross-reactivity or interference between Anti-Interferon Gamma Antibody (Anti-IFNg) and analogues was observed.
Recovery of the ELISA Kit for Anti-Interferon Gamma Antibody (Anti-IFNg)
Matrices listed below were spiked with certain level ofAnti-Interferon Gamma Antibody (Anti-IFNg) and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-Interferon Gamma Antibody (Anti-IFNg) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 78-93 | 87 |
Precision of the ELISA Kit for Anti-Interferon Gamma Antibody (Anti-IFNg)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Interferon Gamma Antibody (Anti-IFNg) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Interferon Gamma Antibody (Anti-IFNg) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>
Linearity of the ELISA Kit for Anti-Interferon Gamma Antibody (Anti-IFNg)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-Interferon Gamma Antibody (Anti-IFNg) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 92-101% | 78-98% | 85-97% | 78-98% |
Stability of the ELISA Kit for Anti-Interferon Gamma Antibody (Anti-IFNg)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the ELISA Kit for Anti-Interferon Gamma Antibody (Anti-IFNg)
1. Prepare all reagents, samples and standards;2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;4. Aspirate and wash 5 times;5. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;6. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle of the ELISA Kit for Anti-Interferon Gamma Antibody (Anti-IFNg)
The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Interferon Gamma Antibody (Anti-IFNg) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Interferon Gamma Antibody (Anti-IFNg) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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比如你用anti-MHC-II，mouse IgG1-FITC，那同型就用非特异性的mouse IgG1-FITC，同样浓度
请问：同型对照应该怎么设？
不买专门的IgG2Bisotypecontrol，而用其他的抗体，
比如mouseanti-humanCD117，Igclass也是IgG2B，可以吗？
阴性对照(Isotype Control)：非特异荧光的强弱取决于抗体浓度、单克隆荧光抗体特异性和纯度，应与试验管抗体相对应。在多色分析时，同型对照应与其它抗体同时使用，以避免补偿造成的误差
血小板体外活化试验：使用正常人活化标本作为阳性质控；使用正常人未活化标本作为阴性质控
血小板自身抗体检测：使用含有已知血小板抗体的血清与血小板孵育，作为阳性质控；使用不含血小板抗体的血清与血小板孵育，作为阴性质控
血小板表面抗原缺失：如巨血小板症血小板表面CD42a/CD42b缺失，血小板无力症血小板表面gpIIb/IIIa，即CD41/CD61缺失或异常。使用正常人标本做阳性对照，抗体的同型对照做阴性对照
英 ['aɪsətaɪp]美 ['aɪsəˌtaɪp]
n. 同型动物（或植物），图形文字，象征性图像;同位型;同模;同号模式
英 ['aisəutaip]
美 ['aisəutaip]
n. 同形像；
同形像统计图
