DescriptionViPrimePLUS Lyophilized qPCR Master Mix is next generation first choice for a fast and easy real-time PCR reaction set up. The master mix is in freeze-dried form which produces stable preparation at room temperature. The freeze-dried master mix contains pure Taq DNA polymerases, MgCl2 and buffers components at optimal concentrations. ViPrimePLUS Lyophilized qPCR Master Mix has same optimal performance as ViPrimePLUS qPCR Master Mix and ViPrimePLUS qPCR Green Master Mix. The lyophilized qPCR master mix has highest sensitivity and specificity to detect the extremely low abundance transcripts with high efficiency. ViPrimePLUS Lyophilized qPCR Master Mix is used with supplied re-suspension buffer and ROX dye; hence hardware platforms that need ROX as a passive reference dye can be used in standard cycling conditions.
Features
- Convenient and rapid real time PCR reaction set up
- Lyophilized form – room temperature stable preparation
- Reliable quantification of low copy number targets
- High PCR sensitivity and specificity
- Compatible with most of the real-time PCR platforms
Kit Component
- 3 x Lyophilized qPCR MasterMix
- 1 x Lyophilized ROX
- 4 x Re-suspension buffer
Master Mixes Selection Guidelines for System CompatibilityTo calibrate a real-time PCR reaction, the various formulations of Master Mixes are available for most of the platforms.
Real-time PCR platforms | ROX re-suspension volume | ROX addition per tube of master mix |
Applied Biosystems 7000, 7700, 7900, 7300 StepOne, StepOnePLUS and ViA7 platforms, Roche capillary Lightcyclers. | 100µl | 20µl |
All Stratagene platforms | 200µl | 15µl |
Applied Biosystems 7500 platform | 700µl | 10µl |
All other machines | Not Required | Not Required |
Ordering Information
Catalog No | Description | Pack Size |
QLMM05 | ViPrimePLUS Lyophilized qPCR Master Mix | 150 reactions |
DownloadManual
ViPrimePLUS Lyophilized qPCR Master Mix
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二抗
抗体组合
免疫组化
免疫沉淀
ELISA
ELISPOT
流式
同型对照
酪胺信号放大
免疫对照/血清/阻断/封闭
Western Blot免疫印迹
免疫缓冲剂/固定/透化
细胞/组织裂解物
细胞核裂解物
细胞裂解物
组织抽提物
转染细胞裂解物
CBA蛋白定量
链霉亲和素
抗体纯化
英 ['aisəutaip]
美 ['aisəutaip]
n. 同形像;
同形像统计图
阴性对照(Isotype Control):非特异荧光的强弱取决于抗体浓度、单克隆荧光抗体特异性和纯度,应与试验管抗体相对应。在多色分析时,同型对照应与其它抗体同时使用,以避免补偿造成的误差
血小板体外活化试验:使用正常人活化标本作为阳性质控;使用正常人未活化标本作为阴性质控
血小板自身抗体检测:使用含有已知血小板抗体的血清与血小板孵育,作为阳性质控;使用不含血小板抗体的血清与血小板孵育,作为阴性质控
血小板表面抗原缺失:如巨血小板症血小板表面CD42a/CD42b缺失,血小板无力症血小板表面gpIIb/IIIa,即CD41/CD61缺失或异常。使用正常人标本做阳性对照,抗体的同型对照做阴性对照