


Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.

ThisantibodyisshippedwithitsantigenFREEofcharge!
- PeptideMDEEEDGAGAEESGQPRSFTQL(C),correspondingtoaminoacidresidues1-22ofratCACNA1G (Accession O54898).Intracellular,N-terminus.
- Westernblotanalysisofratbrainmembranes:1. Anti-CACNA1G(CaV3.1) Antibody(#ACC-021),(1:200).
2.Anti-CACNA1G(CaV3.1)Antibody,preincubatedwiththenegativecontrolantigen.
- ExpressionofCACNA1G inratcerebellumImmunohistochemicalstainingofratcerebellumusing Anti-CACNA1G(CaV3.1) Antibody(#ACC-021).A.CACNA1G immunoreactivity(green)appearsinthemolecularlayer.B.NuclearstainingusingDAPIasthecounterstain(blue).C.MergedimagesAandB.Mol=molecularlayer.Humanmyometrium(1:200)(Blanks,A.M. etal. (2007) J.Physiol. 581, 915.).
- Humanmyometriumcells(1:200)(Blanks,A.M. etal. (2007) J.Physiol. 581, 915.).
- 1.Serrano,J.R.etal.(1999)J.Gen.Physiol.114,185.
- 2.Perez-Reyes,E.(2003)Physiol.Rev.83,117.
- 3.Wain,H.M.etal.(2002)NucleicAcidsRes.30,169.
- 4.Andreasen,D.etal.(2000)Am.J.Physiol.279,F997.
Voltage-dependentCa2+ channelsprovideapathwayforrapidinfluxofCa2+ intocells, whichplaysacrucialroleinbothelectricalandmetabolicsignaling.1
T-typecurrentsaretransducedviachannelproteinsencodedbythreegenesthat composeasubfamilywithintheCaVchannelfamily.2-3
TheactivityofT-typechannelscontributestoseveralknownphysiologicalandpathophysiological phenomenaincludingburstfiringinneurons,pacemakingactivityintheheartandsecretionfromendocrinetissues.2 TherearethreeclonedT-typechannelisoforms.
CACNA1G(CaV3.1)andCACNA1H(CaV3.2)arewidelydistributedwhereastheexpressionofCACNA1I(CaV3.3) isrestrictedtothecentralnervoussystem.2
CACNA1GandCACNA1H arealsoexpressedinthekidney,butlittleisknownabouttheirphysiologicalrolethere.

ExpressionofCaV3.1inmousepelviskidneyjunction.
Immunohistochemicalstainingofmousekidneysectionsusing Anti-CACNA1G(CaV3.1)Antibody (#ACC-021).A.CaV3.1(purple)isdetectedinthepelviskidneyjunction(PKJ).B.HCN3staining(green),aMarkerofpacemakercells,isdetectedinthePKJ.C.SamesectionstainedforCaV3.1(red).D.MergeofBandCpanelsshowshighdegreeofco-localizationbetweenCaV3.1andHCN3.
Adaptedfrom Hurtado,R.etal. (2014)withpermissionof FederationofAmericanSocietiesforExperimentalBIOLOGy.
Anti-CACNA1G(CaV3.1)Antibody(#ACC-021)isahighlyspecificantibodydirectedagainstanepitopeoftheratCaV3.1channel.Theantibody canbeusedinwesternblot,immunocytochemistry,andimmunohistochemistryapplications.IthasbeendesignedtorecognizeCACNA1G fromhuman,rat,andmousesamples.
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请问:同型对照应该怎么设?
不买专门的IgG2Bisotypecontrol,而用其他的抗体,
比如mouseanti-humanCD117,Igclass也是IgG2B,可以吗?
阴性对照(Isotype Control):非特异荧光的强弱取决于抗体浓度、单克隆荧光抗体特异性和纯度,应与试验管抗体相对应。在多色分析时,同型对照应与其它抗体同时使用,以避免补偿造成的误差
血小板体外活化试验:使用正常人活化标本作为阳性质控;使用正常人未活化标本作为阴性质控
血小板自身抗体检测:使用含有已知血小板抗体的血清与血小板孵育,作为阳性质控;使用不含血小板抗体的血清与血小板孵育,作为阴性质控
血小板表面抗原缺失:如巨血小板症血小板表面CD42a/CD42b缺失,血小板无力症血小板表面gpIIb/IIIa,即CD41/CD61缺失或异常。使用正常人标本做阳性对照,抗体的同型对照做阴性对照
选用和特异性抗体同种亚型的,同样荧光素标记的抗体,采用同样的染色步骤染同样浓度的细胞。然后上机检测。如果细胞有非特异性的结合,那同型对照的荧光强度可能会比未染色的阴性对照要高一些。
同型对照只用于那种阳性和阴性之间没有明确分解的情况。如果阳性细胞群非常清楚,是不需要同型对照的。另外,在多色同染的情况下,也不采用同型对照,而是采用FMO(fluorensence minus one)对照。
另外,对于同型对照的使用,也有很多反对意见。就算是同型抗体,也可能会有不同的亲和力,并不一定就能真实反应非特异性的结合。
英 ['aɪsətaɪp]美 ['aɪsəˌtaɪp]
n. 同型动物(或植物),图形文字,象征性图像;同位型;同模;同号模式

