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AntigenIncl.ThisantibodyisshippedwithitsantigenFREEofcharge!
- PeptideMDEEEDGAGAEESGQPRSFTQL(C),correspondingtoaminoacidresidues1-22ofratCACNA1G (Accession O54898).Intracellular,N-terminus.

Westernblotanalysisofratbrainmembranes:1. Anti-CACNA1G(CaV3.1) Antibody(#ACC-021),(1:200).
2.Anti-CACNA1G(CaV3.1)Antibody,preincubatedwiththenegativecontrolantigen.
ExpressionofCACNA1G inratcerebellumImmunohistochemicalstainingofratcerebellumusing Anti-CACNA1G(CaV3.1) Antibody(#ACC-021).A.CACNA1G immunoreactivity(green)appearsinthemolecularlayer.B.NuclearstainingusingDAPIasthecounterstain(blue).C.MergedimagesAandB.Mol=molecularlayer.Humanmyometrium(1:200)(Blanks,A.M. etal. (2007) J.Physiol. 581, 915.).
- Humanmyometriumcells(1:200)(Blanks,A.M. etal. (2007) J.Physiol. 581, 915.).
- 1.Serrano,J.R.etal.(1999)J.Gen.Physiol.114,185.
- 2.Perez-Reyes,E.(2003)Physiol.Rev.83,117.
- 3.Wain,H.M.etal.(2002)NucleicAcidsRes.30,169.
- 4.Andreasen,D.etal.(2000)Am.J.Physiol.279,F997.
Voltage-dependentCa2+ channelsprovideapathwayforrapidinfluxofCa2+ intocells, whichplaysacrucialroleinbothelectricalandmetabolicsignaling.1
T-typecurrentsaretransducedviachannelproteinsencodedbythreegenesthat composeasubfamilywithintheCaVchannelfamily.2-3
TheactivityofT-typechannelscontributestoseveralknownphysiologicalandpathophysiological phenomenaincludingburstfiringinneurons,pacemakingactivityintheheartandsecretionfromendocrinetissues.2 TherearethreeclonedT-typechannelisoforms.
CACNA1G(CaV3.1)andCACNA1H(CaV3.2)arewidelydistributedwhereastheexpressionofCACNA1I(CaV3.3) isrestrictedtothecentralnervoussystem.2
CACNA1GandCACNA1H arealsoexpressedinthekidney,butlittleisknownabouttheirphysiologicalrolethere.

ExpressionofCaV3.1inmousepelviskidneyjunction.
Immunohistochemicalstainingofmousekidneysectionsusing Anti-CACNA1G(CaV3.1)Antibody (#ACC-021).A.CaV3.1(purple)isdetectedinthepelviskidneyjunction(PKJ).B.HCN3staining(green),aMarkerofpacemakercells,isdetectedinthePKJ.C.SamesectionstainedforCaV3.1(red).D.MergeofBandCpanelsshowshighdegreeofco-localizationbetweenCaV3.1andHCN3.
Adaptedfrom Hurtado,R.etal. (2014)withpermissionof FederationofAmericanSocietiesforExperimentalBIOLOGy.
Anti-CACNA1G(CaV3.1)Antibody(#ACC-021)isahighlyspecificantibodydirectedagainstanepitopeoftheratCaV3.1channel.Theantibody canbeusedinwesternblot,immunocytochemistry,andimmunohistochemistryapplications.IthasbeendesignedtorecognizeCACNA1G fromhuman,rat,andmousesamples.
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选用和特异性抗体同种亚型的,同样荧光素标记的抗体,采用同样的染色步骤染同样浓度的细胞。然后上机检测。如果细胞有非特异性的结合,那同型对照的荧光强度可能会比未染色的阴性对照要高一些。
同型对照只用于那种阳性和阴性之间没有明确分解的情况。如果阳性细胞群非常清楚,是不需要同型对照的。另外,在多色同染的情况下,也不采用同型对照,而是采用FMO(fluorensence minus one)对照。
另外,对于同型对照的使用,也有很多反对意见。就算是同型抗体,也可能会有不同的亲和力,并不一定就能真实反应非特异性的结合。
手头上,有eBioscience家的同型抗体,为PE标记(mouseIgG1,k),说明书写使用为2.5ug/test。
按照说明书的浓度加入后结果发现,同型对照抗体组比实验组和阴性对照组(不表达CD80)荧光强度要高,很明确我的同型对照加入的剂量多了,但正确的加法是多少?按照质量算吗?请高手指教,非常感谢!!

