Background5T4isaN-glycosylatedtransmembrane72kDa(420AA)glycoproteincontainingsevenleucine-richrepeatregions.5T4isoftenreferredtoasanoncofetalantigenduetoitsexpressioninfetaltrophoblastwhereitwasfirstdiscoveredortrophoblastglycoprotein(TPBG).5T4oncofetalantigenisdefinedbyamonoclonalantibodyoriginallyraisedagainsthumanplacentaltrophoblastandwhichrecognizesa72kDglycoproteinexpressedinavarietyofdifferentcarcinomasbutdetectedonlyatverylowlevelsinnormalepithelia.Patternsofexpressionof5T4oncofetalantigenincolorectalcarcinomasindicateasignificantassociationwithtumormetastasis.ByIHC,71%ofthecarcinomasdemonstratepositive5T4immunoreactivityinadenocarcinomacellsand/orassociatedstromaltissue.5T4isfoundintumorsincludingthecolorectal,ovarian,andgastric.Itsexpressionisusedasaprognosticaidinthesecases.5T4iswidespreadinmalignanttumorsthroughoutthevariousstagesoftheirdevelopment.Inassessingthe5T4phenotypeithasbeendeterminedthatthereisasignificantcorrelationbetween5T4expressionandadvancedstageofdisease(FIGOstagesIIIandIV)(P<0.001)=""and=""with=""poorly=""differentiated=""tumors=""(p="0.036)"compared=""to=""well=""or=""moderately=""differentiated=""tumors.=""patients=""with=""tumors=""expressing=""5t4=""were=""are=""likely=""to=""respond=""well=""to=""adjuvant=""therapy=""and=""have=""a=""significantly=""worse=""outlook=""in=""terms=""of=""survival=""and=""disease-free=""interval.="">

Synonyms:Trophoblastglycoprotein,TPBG,5T4oncofetalantigen,5T4oncotrophoblastglycoprotein,5T4oncofetaltrophoblastglycoprotein,M6P1

Source

Immunogen:Syntheticpeptidederivedfromhuman5T4oncofetalantigenprotein

Product

ProductForm:AffinityPurified

Formulation:Providedassolutioninphosphatebufferedsalinewith0.08%sodiumazide

PurificationMethod:AntigenImmunoaffinityPurification

Concentration:Seevialforconcentration

ApplicationsThisantibodycanbeusedforimmunohistochemistryonformalin/paraffinsections(5-10µg/ml).Optimalconcentrationshouldbeevaluatedbyserialdilutions.

FunctionalAnalysis:Immunohistochemistry

PositiveControl:OvarianCancer,gastriccancer,colorectalcancer.

StorageProductshouldbestoredat-20ºC.Aliquottoavoidfreeze/thawcycles

ProductStABIlity:Productsarestableforoneyearfrompurchasewhenstoredproperly

ShippingConditions:Shipatambienttemperature,freezeuponarrival

CautionThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Itmaycontainhazardousingredients. PleaserefertotheSafetyDataSheets(SDS)foradditionalinformationandproperhandlingprocedures.Disposeproductremaindersaccordingtolocalregulations.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-Mubio​acceptsnoliabilityforanyinaccuraciesoromissionsinthisinformation.

References1.Yoshihara,S.,etal."5T4glycoproteinregulatesthesensoryinput-dependentdevelopmentofaspecificsubtypeofnewborninterneuronsinthemouseolfactorybulb."J.Neurosci.2012,32,2217-2226.2.Southgate,T.D.,etal."CXCR4mediatedchemotaxisisregulatedby5T4oncofetalglycoproteininmouseembryoniccells."PLoSOne2010,5,e9982.3.Shingler,W.H.,etal."IdentificationandfunctionalvalidationofMHCclassIepitopesinthetumor-associatedantigen5T4."Int.Immunol.2008,20,1057-1066.

ProteinReference(s)

DatabaseName:SwissProt

Accessionnumber:Q13641

SpeciesAccession:Human

SafetyDatasheet(s)forthisproduct:NM_SodiumAzide/wp-content/uploads/SDS/AntibodySDSwithSodiumAzideNoridic-MUbio.pdf