BackgroundAdipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines (reviewed in 1). One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-?-related proteins (CTRPs) 1-7 (2). Unlike the CTRPs, which are expressed in a wide variety of tissues, adiponectin is reported to be expressed exclusively by differentiated adipocytes (3). These proteins are thought to act mainly on liver and muscle tissue to control glucose and lipid metabolism. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene (4). It is present in high levels in normal human plasma, but is reduced in obese subjects and often in those with increased insulin resistance and type 2 diabetes, suggesting that adiponectin may be a useful pharmacological target in various metabolic diseases (5).

Synonyms: ACRP30, adipocyte complement-related protein 30 (NT)

Source

Immunogen: Rabbit polyclonal adiponectin antibody was raised against a 15 amino acid peptide from near the amino terminus of human adiponectin.

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.02% sodium azide

Purification Method: Antigen Immunoaffiinity Purification

Concentration: See vial for concentration

ApplicationsAdiponectin antibody can be used for the detection of adiponectin by Western blot at 1 ug/ml.

Functional Analysis: Western Blotting

Positive Control: Positive Control Rat brain cell lysateA secreted protein synthesized exclusively by adipocytes.6

StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Stability: Products are stable for one year from purchase when stored properly

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References1) Fantuzzi G.Adipose tissue, adipokines, andinflammation.J. Allergy Clin. Immunol. 2005; 115:911-9.2) Tsao T-S, Lodish HF, and Fruebis J.ACRP30, a newhormone controlling fat and glucose metabolism.Euro. J.Pharmacol. 2002; 440:213-21.3.) Wong GW, Wang J, Hug C, et al.A family of Acrp30/adiponectin structural and functional paralogs.Proc. Natl.Acad. Sci. USA 2004; 101:10302-7.4) Shapiro L and Scherer PE.The crystal structure of acomplement-1q family protein suggests an evolutionary linkto tumor necrosis factor.Curr. Biol. 1998; 8:335-8.5) Lihn AS, Pedersen SB, and Richelsen B.Adiponectin: action, regulation and association to insulin sensitivity. Obes. Rev. 2005; 6:13-21. 6) UniProtKB/Swiss-Prot entry Q15848, http://au.expasy.org/uniprot/Q15848, Accessed February 27, 2007.

Protein Reference(s)

Database Name: UniProt

Accession number: Q15848

Species Accession: Human

Safety Datasheet(s) for this product:NM_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium Azide Noridic-MUbio.pdf