| Product Type | Buffers and Reagents |
| Units | 250 ml |
| Application | Immunohistochemistry (paraffin) |
BackgroundOne of the most effective solutions for antigen unmasking is an unmasking fluid on glycol basis, one of which is our "Unmasking Fluid G" (Code No. DE007). Unmasking Fluid G makes antigens detectable after formaldehyde fixation of tissues, even after prolonged fixation times. Usage of this Unmasking Fluid G usually guarantees also a good preservation of cellular structures. In this respect it is superior to unmasking fluids on citrate or urea basis, which might have their own advantages in detection of certain antigens.Aldehyde fixation (Formaldehyde fixation) of tissues generally causes cross-linkage of protein chains and results in masking of antigens present in the treated tissue. This makes the antigen epitopes undetectable for many antibodies applied for immunohistochemistry. Mainly monoclonal antibodies, but also mono-specific polyclonal antibodies will react with reduced binding or no binding at all. Since Formaldehyde fixation is the most important fixation method in routine histology, high temperature unmasking methods for immunohistochemistry, as developed during recent years, are very important tools for cell and tissue research and pathology.
Product250 ml 4-times concentrated solution
Purification Method: 250 ml 4-times concentrated solution
ApplicationsIHC(P)
Working Concentration: (4 x concentrated solution) 1:4
Positive Control: Human: AMACR, ATF-2 (Thr71), B cells (CD20), BAX, bcl-2 oncoprotein, CD34 (QBEND10), CEA, Cytokeratins, c-erbB-2, Desmin, HEA125, Ki-67, (MAC387), MAP-Kinase (1/2), Melan-A (MART-1) neurofilaments, NSE, Estrogen Receptor, p53 protein, p63 protein, p170 glycoprotein, P504S, PSA, Synaptophysin, Thyroglobulin, TTF-1, T cells (CD3eand CD45RO), Vimentin (V9 and 3B4), von Willebrand-Factor etc. Rat: CD4 (W3/25), CD8 (OX-8), CD11b (OX-42), CD25 (OX-39), CD45RA (OX-33), Macrophages (ED1, ED2, MAC387)
Storage2-25°C
CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
References
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比如你用anti-MHC-II，mouse IgG1-FITC，那同型就用非特异性的mouse IgG1-FITC，同样浓度
请问：同型对照应该怎么设？
不买专门的IgG2Bisotypecontrol，而用其他的抗体，
比如mouseanti-humanCD117，Igclass也是IgG2B，可以吗？
阴性对照(Isotype Control)：非特异荧光的强弱取决于抗体浓度、单克隆荧光抗体特异性和纯度，应与试验管抗体相对应。在多色分析时，同型对照应与其它抗体同时使用，以避免补偿造成的误差
血小板体外活化试验：使用正常人活化标本作为阳性质控；使用正常人未活化标本作为阴性质控
血小板自身抗体检测：使用含有已知血小板抗体的血清与血小板孵育，作为阳性质控；使用不含血小板抗体的血清与血小板孵育，作为阴性质控
血小板表面抗原缺失：如巨血小板症血小板表面CD42a/CD42b缺失，血小板无力症血小板表面gpIIb/IIIa，即CD41/CD61缺失或异常。使用正常人标本做阳性对照，抗体的同型对照做阴性对照
英 ['aɪsətaɪp]美 ['aɪsəˌtaɪp]
n. 同型动物（或植物），图形文字，象征性图像;同位型;同模;同号模式
英 ['aisəutaip]
美 ['aisəutaip]
n. 同形像；
同形像统计图
