| ProductType | ControlLysate |
| Units | 100µl |
| Application | WesternBlotting |
BackgroundEndothelialcelldifferentiationgene-2(EDG-2)receptorisahighaffinityreceptorforlysophosphatidicacid(LPA).EDG-2,alsoknownaslysophospholipidreceptorA1,Vzg-1,mrec1.3orGPCR26,likeallotherEDGreceptors,couplemultiple(3ormore)typesofGproteinsandtransducedecreasesinc[AMP]ithroughGiandincreasesin[Ca2+]ibyaugmentingphospholipaseCthroughGq/11andbeta/gammadimersandbyinductionofPI3kinase,p125FAK,phospholipaseDbyactivatingrhothroughG12/13.HumanEDG-2ispresentinhighlevelsonoligodendrocytesandcertainhumanmalignantTcelllines.EDG-2receptors(withEDG-4)mayplayaroleinprotectingcardiomyocytesfromapoptosisinducedbyhypoxiaandandrenergicstimulation.
Synonyms:EDG-2transfectedcelllysate
Product
ProductForm:Unconjugated
Formulation:Providedin10%glycerol,0.063MTris-HCl(pH6.8),2%SDSand0.002%bromophenolblue,5%2-mercaptoethanol
Concentration:Seevialforconcentration
ApplicationsForuseasapositivecontrolwithExalpha"sEDG2(LPA1)polyclonalantibody(Cat.No.C174P)forWesternblotanalysis.
FunctionalAnalysis:WesternBlotting
StorageProductshouldbestoredat-20ºC.Aliquottoavoidfreeze/thawcycles
ProductStABIlity:Productsarestableforoneyearfrompurchasewhenstoredproperly
ShippingConditions:Shipatambienttemperature,freezeuponarrival
CautionThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Itmaycontainhazardousingredients. PleaserefertotheSafetyDataSheets(SDS)foradditionalinformationandproperhandlingprocedures.Disposeproductremaindersaccordingtolocalregulations.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-Mubioacceptsnoliabilityforanyinaccuraciesoromissionsinthisinformation.
ProteinReference(s)
DatabaseName:UniProt
Accessionnumber:Q92633
SafetyDatasheet(s)forthisproduct:NM_Glycerol/wp-content/uploads/SDS/SDSAntibodieswithglycerolNordic-MUbio.pdf
ebiomall.com
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比如你用anti-MHC-II，mouse IgG1-FITC，那同型就用非特异性的mouse IgG1-FITC，同样浓度
请问：同型对照应该怎么设？
不买专门的IgG2Bisotypecontrol，而用其他的抗体，
比如mouseanti-humanCD117，Igclass也是IgG2B，可以吗？
阴性对照(Isotype Control)：非特异荧光的强弱取决于抗体浓度、单克隆荧光抗体特异性和纯度，应与试验管抗体相对应。在多色分析时，同型对照应与其它抗体同时使用，以避免补偿造成的误差
血小板体外活化试验：使用正常人活化标本作为阳性质控；使用正常人未活化标本作为阴性质控
血小板自身抗体检测：使用含有已知血小板抗体的血清与血小板孵育，作为阳性质控；使用不含血小板抗体的血清与血小板孵育，作为阴性质控
血小板表面抗原缺失：如巨血小板症血小板表面CD42a/CD42b缺失，血小板无力症血小板表面gpIIb/IIIa，即CD41/CD61缺失或异常。使用正常人标本做阳性对照，抗体的同型对照做阴性对照
英 ['aɪsətaɪp]美 ['aɪsəˌtaɪp]
n. 同型动物（或植物），图形文字，象征性图像;同位型;同模;同号模式
英 ['aisəutaip]
美 ['aisəutaip]
n. 同形像；
同形像统计图
