ProductType | ControlLysate |
Units | 100µl |
Application | WesternBlotting |
BackgroundEndothelialcelldifferentiationgene-2(EDG-2)receptorisahighaffinityreceptorforlysophosphatidicacid(LPA).EDG-2,alsoknownaslysophospholipidreceptorA1,Vzg-1,mrec1.3orGPCR26,likeallotherEDGreceptors,couplemultiple(3ormore)typesofGproteinsandtransducedecreasesinc[AMP]ithroughGiandincreasesin[Ca2+]ibyaugmentingphospholipaseCthroughGq/11andbeta/gammadimersandbyinductionofPI3kinase,p125FAK,phospholipaseDbyactivatingrhothroughG12/13.HumanEDG-2ispresentinhighlevelsonoligodendrocytesandcertainhumanmalignantTcelllines.EDG-2receptors(withEDG-4)mayplayaroleinprotectingcardiomyocytesfromapoptosisinducedbyhypoxiaandandrenergicstimulation.
Synonyms:EDG-2transfectedcelllysate
Product
ProductForm:Unconjugated
Formulation:Providedin10%glycerol,0.063MTris-HCl(pH6.8),2%SDSand0.002%bromophenolblue,5%2-mercaptoethanol
Concentration:Seevialforconcentration
ApplicationsForuseasapositivecontrolwithExalpha"sEDG2(LPA1)polyclonalantibody(Cat.No.C174P)forWesternblotanalysis.
FunctionalAnalysis:WesternBlotting
StorageProductshouldbestoredat-20ºC.Aliquottoavoidfreeze/thawcycles
ProductStABIlity:Productsarestableforoneyearfrompurchasewhenstoredproperly
ShippingConditions:Shipatambienttemperature,freezeuponarrival
CautionThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Itmaycontainhazardousingredients. PleaserefertotheSafetyDataSheets(SDS)foradditionalinformationandproperhandlingprocedures.Disposeproductremaindersaccordingtolocalregulations.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-Mubioacceptsnoliabilityforanyinaccuraciesoromissionsinthisinformation.
ProteinReference(s)
DatabaseName:UniProt
Accessionnumber:Q92633
SafetyDatasheet(s)forthisproduct:NM_Glycerol/wp-content/uploads/SDS/SDSAntibodieswithglycerolNordic-MUbio.pdf
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二抗
抗体组合
免疫组化
免疫沉淀
ELISA
ELISPOT
流式
同型对照
酪胺信号放大
免疫对照/血清/阻断/封闭
Western Blot免疫印迹
免疫缓冲剂/固定/透化
细胞/组织裂解物
细胞核裂解物
细胞裂解物
组织抽提物
转染细胞裂解物
CBA蛋白定量
链霉亲和素
抗体纯化
英 ['aisəutaip]
美 ['aisəutaip]
n. 同形像;
同形像统计图
阴性对照(Isotype Control):非特异荧光的强弱取决于抗体浓度、单克隆荧光抗体特异性和纯度,应与试验管抗体相对应。在多色分析时,同型对照应与其它抗体同时使用,以避免补偿造成的误差
血小板体外活化试验:使用正常人活化标本作为阳性质控;使用正常人未活化标本作为阴性质控
血小板自身抗体检测:使用含有已知血小板抗体的血清与血小板孵育,作为阳性质控;使用不含血小板抗体的血清与血小板孵育,作为阴性质控
血小板表面抗原缺失:如巨血小板症血小板表面CD42a/CD42b缺失,血小板无力症血小板表面gpIIb/IIIa,即CD41/CD61缺失或异常。使用正常人标本做阳性对照,抗体的同型对照做阴性对照