Clone | B-A38 |
Isotype | IgG1 |
ProductType | MonoclonalAntibodyPrimaryAntibodies |
Units | 5ml |
Host | Mouse |
Speciesreactivity | Human |
Application | Immunohistochemistry(frozen)Immunohistochemistry(paraffin) |
BackgroundTheantibodyB-A38(likeB-B4)stainsallplasmacellsandallplasmacelllines,whichhavebeentestedsofar.Nootherleukocytesarestainedinperipheralbloodorbonemarrow.However,someALLcelllinesexpressCD138weekly,andsomeepitheliaandendotheliamaybestainedinimmunohistochemistry.Syndecan1,CD138,isaheparan-richglycoprotein(MW200kDa),whichisexpressedonthecellmembraneofplasmacells.CD138functionsasareceptorforextracellularmatrixproteinslikecollagen,fibronectinandthrombospondin.HumanCD138,Syndecan1.
Source
Immunogen:U266cellline
ProductAntibodysolutioninstABIlizingphosphatebufferpH7.3.ContainsBSAand0.09%sodiumazide**.Thevolumeissufficientforatleast50immunohistochemicaltests(100µlworkingsolution/test).Useappropriateantibodydiluente.g.BIOLOGOArt.No.PU002,iffurtherdilutionisrequired.
PurificationMethod:AntibodysolutioninstabilizingphosphatebufferpH7.3.ContainsBSAand0.09%sodiumazide**.Thevolumeissufficientforatleast50immunohistochemicaltests(100µlworkingsolution/test).Useappropriateantibodydiluente.g.BIOLOGOArt.No.PU002,iffurtherdilutionisrequired.
SecondaryReagents:WerecommendtheuseofBIOLOGO"sUniversalStainingSystemDAB(Art.No.DA005)orAEC(Art.-No.AE005).
Specificity
SpeciesReactivity:Human,othersnottested
ApplicationsIHC(C,P)
IncubationTime:60minatRT
WorkingConcentration:(RTU)neat
Pre-Treatment:NoproteasenecessaryforIHC,butheatpre-treatmentwithcitratebuffer(Art.No.DE000)orEDTAbufferpH9(Art.No.DE006)mayenhancestainingintensity.
PositiveControl:Tonsil
Storage2-8°C
CautionThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Itmaycontainhazardousingredients. PleaserefertotheSafetyDataSheets(SDS)foradditionalinformationandproperhandlingprocedures.Disposeproductremaindersaccordingtolocalregulations.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-Mubioacceptsnoliabilityforanyinaccuraciesoromissionsinthisinformation.
References1.BorsetM.,etal.(1993)LackofIL-1secretionfromhumanmyelomacellshighlypurifiedbyimmunomagneticseparation.Br.J.Haem.85;446ff.2.WijdenesJ.,etal.(1996)Aplasmocyteselectivemonoclonalantibody(B-B4)recognisesSyndecan-1.Br.J.Haem.94;318-323.3.Inki.P.andJalkanenM.(1996)Theroleofsyndecan-1inmalignancies.Ann.Med.28(1);63-67.4.AnttonenA.,etal.(1999)Syndecan-1expressionhasprognosticsignificanceinheadandneckcarcinoma.BritJ.Cancer79;558-564.5.YangY,MacLeodV,DaiY,Khotskaya-SampleY,ShriverZ,VenkataramanG,SasisekharanR,NaggiA,TorriG,CasuB,VlodavskyI,SuvaLJ,EpsteinJ,YaccobyS,ShaughnessyJDJr,BarlogieB,SandersonRD.(2007)Thesyndecan-1heparansulfateproteoglycan
SafetyDatasheet(s)forthisproduct:NM_SodiumAzide/wp-content/uploads/SDS/AntibodySDSwithSodiumAzideNoridic-MUbio.pdf
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二抗
抗体组合
免疫组化
免疫沉淀
ELISA
ELISPOT
流式
同型对照
酪胺信号放大
免疫对照/血清/阻断/封闭
Western Blot免疫印迹
免疫缓冲剂/固定/透化
细胞/组织裂解物
细胞核裂解物
细胞裂解物
组织抽提物
转染细胞裂解物
CBA蛋白定量
链霉亲和素
抗体纯化
英 ['aisəutaip]
美 ['aisəutaip]
n. 同形像;
同形像统计图
阴性对照(Isotype Control):非特异荧光的强弱取决于抗体浓度、单克隆荧光抗体特异性和纯度,应与试验管抗体相对应。在多色分析时,同型对照应与其它抗体同时使用,以避免补偿造成的误差
血小板体外活化试验:使用正常人活化标本作为阳性质控;使用正常人未活化标本作为阴性质控
血小板自身抗体检测:使用含有已知血小板抗体的血清与血小板孵育,作为阳性质控;使用不含血小板抗体的血清与血小板孵育,作为阴性质控
血小板表面抗原缺失:如巨血小板症血小板表面CD42a/CD42b缺失,血小板无力症血小板表面gpIIb/IIIa,即CD41/CD61缺失或异常。使用正常人标本做阳性对照,抗体的同型对照做阴性对照