| Clone | B-A38 |
| Isotype | IgG1 |
| ProductType | MonoclonalAntibodyPrimaryAntibodies |
| Units | 5ml |
| Host | Mouse |
| Speciesreactivity | Human |
| Application | Immunohistochemistry(frozen)Immunohistochemistry(paraffin) |
BackgroundTheantibodyB-A38(likeB-B4)stainsallplasmacellsandallplasmacelllines,whichhavebeentestedsofar.Nootherleukocytesarestainedinperipheralbloodorbonemarrow.However,someALLcelllinesexpressCD138weekly,andsomeepitheliaandendotheliamaybestainedinimmunohistochemistry.Syndecan1,CD138,isaheparan-richglycoprotein(MW200kDa),whichisexpressedonthecellmembraneofplasmacells.CD138functionsasareceptorforextracellularmatrixproteinslikecollagen,fibronectinandthrombospondin.HumanCD138,Syndecan1.
Source
Immunogen:U266cellline
ProductAntibodysolutioninstABIlizingphosphatebufferpH7.3.ContainsBSAand0.09%sodiumazide**.Thevolumeissufficientforatleast50immunohistochemicaltests(100µlworkingsolution/test).Useappropriateantibodydiluente.g.BIOLOGOArt.No.PU002,iffurtherdilutionisrequired.
PurificationMethod:AntibodysolutioninstabilizingphosphatebufferpH7.3.ContainsBSAand0.09%sodiumazide**.Thevolumeissufficientforatleast50immunohistochemicaltests(100µlworkingsolution/test).Useappropriateantibodydiluente.g.BIOLOGOArt.No.PU002,iffurtherdilutionisrequired.
SecondaryReagents:WerecommendtheuseofBIOLOGO"sUniversalStainingSystemDAB(Art.No.DA005)orAEC(Art.-No.AE005).
Specificity
SpeciesReactivity:Human,othersnottested
ApplicationsIHC(C,P)
IncubationTime:60minatRT
WorkingConcentration:(RTU)neat
Pre-Treatment:NoproteasenecessaryforIHC,butheatpre-treatmentwithcitratebuffer(Art.No.DE000)orEDTAbufferpH9(Art.No.DE006)mayenhancestainingintensity.
PositiveControl:Tonsil
Storage2-8°C
CautionThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Itmaycontainhazardousingredients. PleaserefertotheSafetyDataSheets(SDS)foradditionalinformationandproperhandlingprocedures.Disposeproductremaindersaccordingtolocalregulations.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-Mubioacceptsnoliabilityforanyinaccuraciesoromissionsinthisinformation.
References1.BorsetM.,etal.(1993)LackofIL-1secretionfromhumanmyelomacellshighlypurifiedbyimmunomagneticseparation.Br.J.Haem.85;446ff.2.WijdenesJ.,etal.(1996)Aplasmocyteselectivemonoclonalantibody(B-B4)recognisesSyndecan-1.Br.J.Haem.94;318-323.3.Inki.P.andJalkanenM.(1996)Theroleofsyndecan-1inmalignancies.Ann.Med.28(1);63-67.4.AnttonenA.,etal.(1999)Syndecan-1expressionhasprognosticsignificanceinheadandneckcarcinoma.BritJ.Cancer79;558-564.5.YangY,MacLeodV,DaiY,Khotskaya-SampleY,ShriverZ,VenkataramanG,SasisekharanR,NaggiA,TorriG,CasuB,VlodavskyI,SuvaLJ,EpsteinJ,YaccobyS,ShaughnessyJDJr,BarlogieB,SandersonRD.(2007)Thesyndecan-1heparansulfateproteoglycan
SafetyDatasheet(s)forthisproduct:NM_SodiumAzide/wp-content/uploads/SDS/AntibodySDSwithSodiumAzideNoridic-MUbio.pdf
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
比如你用anti-MHC-II，mouse IgG1-FITC，那同型就用非特异性的mouse IgG1-FITC，同样浓度
英 ['aɪsətaɪp]美 ['aɪsəˌtaɪp]
n.
同型动物（或植物），图形文字，象征性图像;同位型;同模;同号模式
网络
同型;同种型;模式标本
双语例句
Study on the Action of Decreasing level of Isotype Cysteine for High Blood Viscosity
降低同型半胱氨酸水平对高黏血症作用的研究
英 ['aisəutaip]
美 ['aisəutaip]
n. 同形像；
同形像统计图
阴性对照(Isotype Control)：非特异荧光的强弱取决于抗体浓度、单克隆荧光抗体特异性和纯度，应与试验管抗体相对应。在多色分析时，同型对照应与其它抗体同时使用，以避免补偿造成的误差
血小板体外活化试验：使用正常人活化标本作为阳性质控；使用正常人未活化标本作为阴性质控
血小板自身抗体检测：使用含有已知血小板抗体的血清与血小板孵育，作为阳性质控；使用不含血小板抗体的血清与血小板孵育，作为阴性质控
血小板表面抗原缺失：如巨血小板症血小板表面CD42a/CD42b缺失，血小板无力症血小板表面gpIIb/IIIa，即CD41/CD61缺失或异常。使用正常人标本做阳性对照，抗体的同型对照做阴性对照
选用和特异性抗体同种亚型的，同样荧光素标记的抗体，采用同样的染色步骤染同样浓度的细胞。然后上机检测。如果细胞有非特异性的结合，那同型对照的荧光强度可能会比未染色的阴性对照要高一些。
同型对照只用于那种阳性和阴性之间没有明确分解的情况。如果阳性细胞群非常清楚，是不需要同型对照的。另外，在多色同染的情况下，也不采用同型对照，而是采用FMO（fluorensence minus one）对照。
另外，对于同型对照的使用，也有很多反对意见。就算是同型抗体，也可能会有不同的亲和力，并不一定就能真实反应非特异性的结合。
英 ['aɪsətaɪp]美 ['aɪsəˌtaɪp]
n. 同型动物（或植物），图形文字，象征性图像;同位型;同模;同号模式
