| Clone | FG4R |
| Isotype | IgG2b |
| ProductType | MonoclonalAntibody |
| Units | 100µg |
| Host | Mouse |
| Application | DotblotELISAImmunoprecipitationWesternBlotting |
BackgroundEpitopetagsareausefulwaytoidentifyprotiensinavarietyofcelltypes,studythetopologyofproteinsandproteincomplexes,identifyassociatedproteins,andcharacterizenewlyidentified,lowabundanceorpoorlyimmunogenicproteinswhenproteinspecificantibodiesarenotavailable.TaggingwithExalpha"sDYKDDDDKFLAGantibodymaybedoneattheN-terminus,N-terminusprecededbyamethionineresidue,C-terminus,andininternalpositionsofthetargetprotein.Thesmallsizeofthisepitopetaganditshighhydrophilicitytendtodecreasethepossibilityofinterferencewithproteinexpression,proteolyticmaturation,antigenicityandfunction.Theenterokinasecleavagesiteallowsittobecompletelyremovedfromthepurifiedfusionproteins.ReactswithFLAGtaggedfusionproteins.
Source
Immunogen:DYKDDDDK(FLAG)syntheticpeptideconjugatedtoKLH
Product
ProductForm:Unconjugated
Formulation:10mMPBS,pH7.2,with0.05%SodiumAzide
PurificationMethod:ProteinA/GChromatography
Concentration:Seevialforconcentration
ApplicationsWB(withECL):1:1000-3000dilution(incubateforonehouratroomtemperature)IS:1:500-2000dilutionForbestresultswithotherassays(e.g.:Dot,ELISA,IP,etc),pleasedetermineoptimalworkingdilutionbytitrationtest
FunctionalAnalysis:WesternBlotting
PositiveControl:RecognizestheN-terminal,C-terminalorinternalDYKDDDDK-taggedfusionproteinsRecognizes1-5ngofFLAGtaggedproteinsinwBwithECLdetection
StorageProductshouldbestoredat-20ºC.Aliquottoavoidfreeze/thawcycles
ProductStABIlity:Productsarestableforoneyearfrompurchasewhenstoredproperly
ShippingConditions:Shipatambienttemperature,freezeuponarrival
CautionThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Itmaycontainhazardousingredients. PleaserefertotheSafetyDataSheets(SDS)foradditionalinformationandproperhandlingprocedures.Disposeproductremaindersaccordingtolocalregulations.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-Mubioacceptsnoliabilityforanyinaccuraciesoromissionsinthisinformation.
SafetyDatasheet(s)forthisproduct:NM_SodiumAzide/wp-content/uploads/SDS/AntibodySDSwithSodiumAzideNoridic-MUbio.pdf
ebiomall.com
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比如你用anti-MHC-II，mouse IgG1-FITC，那同型就用非特异性的mouse IgG1-FITC，同样浓度
请问：同型对照应该怎么设？
不买专门的IgG2Bisotypecontrol，而用其他的抗体，
比如mouseanti-humanCD117，Igclass也是IgG2B，可以吗？
阴性对照(Isotype Control)：非特异荧光的强弱取决于抗体浓度、单克隆荧光抗体特异性和纯度，应与试验管抗体相对应。在多色分析时，同型对照应与其它抗体同时使用，以避免补偿造成的误差
血小板体外活化试验：使用正常人活化标本作为阳性质控；使用正常人未活化标本作为阴性质控
血小板自身抗体检测：使用含有已知血小板抗体的血清与血小板孵育，作为阳性质控；使用不含血小板抗体的血清与血小板孵育，作为阴性质控
血小板表面抗原缺失：如巨血小板症血小板表面CD42a/CD42b缺失，血小板无力症血小板表面gpIIb/IIIa，即CD41/CD61缺失或异常。使用正常人标本做阳性对照，抗体的同型对照做阴性对照
英 ['aɪsətaɪp]美 ['aɪsəˌtaɪp]
n. 同型动物（或植物），图形文字，象征性图像;同位型;同模;同号模式
英 ['aisəutaip]
美 ['aisəutaip]
n. 同形像；
同形像统计图
