BackgroundThe CD3 epitope is expressed on the epsilon chain of the CD3/T cell antigen receptor (TcR) complex. CD3 is present on 65-85% of thymocytes and has a mitogenic effect on peripheral blood T cells.CD3 indentifies human T cellsexpressing the 22-28,000 M.W. surface antigen.CD4 identifies human helper/inducer T cells expressing the 60,000 M. W. surface antigen (HLA class II reactive). CD4 is present in low density on monocytes.Identification of CD8on human cytotoxic/ suppressor T cells expressing the 32 and 43,000 M.W. surface antigens.

Synonyms: CD3/4/8

Source

Immunogen: CD3=Derived from the hybridization of mouse NS-1 myeloma celss with spleen cells from BALB/c mice immunized with human thymocytes. CD4=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes. CD8=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.

Product

Product Form: RPE-Cy-5

Formulation: Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein

Purification Method: Affinity Chromatography

Concentration: Titered for flow cytometry

ApplicationsPBMC: Add 15 µl of 3-ColorTM antibody reagent/10^6 PBMC in 100 µl PBS.Mix gently and incubate for 15 minutes at 2 ° to 8° C.Wash twice with PBS. Wash twice with PBS or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD:Add 15 µl of Exalpha Biological's 3-ColorTM antibody reagent MAB/100 µl of whole blood.Mix gently and incubate for 15 minutes at room temperature 20°C.Lyse the whole blood.Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescenceanalysis with a flow cytometer or microscope.

Functional Analysis: Flow Cytometry Staining

StorageProduct should be stored at 4-8ºC. DO NOT FREEZE

Product Stability: Reagents are stable for the period shown on the vial label when stored properly

Shipping Conditions: Room Temperature

CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References1. Orfao A, González de Buitrago JM La citometría de flujo en el laboratorio clínico. Sociedad española de bioquímica clínica y patología Molecular 1995.2. Stetler-Stevenson M. Flow cytometry analysis of lymphomas and lymphoproliferative disorders. Semin Hematol 2001 Apr;38(2):111-23.3. Menéndez P, et al. Comparison between a lyse-and-then-wash method and a lyse-non-wash technique for the enumeration of CD34+ hematopoietic progenitor cells. Cytometry (Comm. Clin. Cytometry) 34: 264-271 (1998)4. GratamaJW, Menéndez P, Kraan J, Orfao A. Loss of CD34+ hematopoietic progenitor cells due to washing can be reduced by the use of fixative-free erytrocyte lysing reagents. J Immunol. Methods 239: 13-23 (2000)5. Protection of Laboratory Workers from occupationally acquired infections. Second edition; approved guideline (2001). Villanova PA: National Committee for Clinical Laboratory Standards; Document M29-A2.6. Procedures for the collection of diagnostic blood specimens by venipuncture- approved standard; Fifth edition (2003). Wayne PA: National Committee for Clinical Laboratory Standards; Document H3-A5.7. Clinical applications of flow cytometry: Quality assurance and immunophenotyping of lymphocytes; approved guideline (1998). Wayne PA: National Committee for Clinical Laboratory Standards; Document H42-A.8. Lima M et al. TCRαβ+/CD4+ Large Granular Lymphocytosis. A new Clonal T-Cell Lymphoprolipherative Disorder. American Journal of Pathology, 163 (2):763-771 (2003)9. Gorczyza W. et al. An approach to diagnosis of T-cell lymphoprolipherative disorders by flow cytometry. Cytometry (Clinical cytometry) 50:177-190 (2002)10. Braylan RC, Orfao A, Borowitz MJ, Davis BH. Optimal number of reagents required to evaluate hematolymphoid neoplasias: results of an international consensus meeting. Cytometry 46: 23-7 (2001)11. Jennings CD, Foon KA. Recent advances in flow cytometry: application to the diagnosis of hematologic malignancy. Blood 90(8): 2863-2892 (1997)12. Reichert et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopathol 60:190-208 (1991) 13. Prince HK et al. Influence of racial background on the distribution of T-cell subsets and Leu-11 positive lymphocytes in healthyblood donors. Diagn Immunol. 3: 33-39 (1985) 14. Kotylo PK et al. Reference ranges for lymphocyte subsets in pediatric patients. Am J Clin Pathol 100:111-5 (1993)Cy-5Portions of this product is manufactured under license from Carnegie Mellon University, U.S. Patent Number 5,268,486.

Protein Reference(s)

Database Name: UniProt

Accession number: P07766, P01730, P01732

Species Accession: Human

Safety Datasheet(s) for this product:NM_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium Azide Noridic-MUbio.pdf