LyophilizedPowderThisproductisfreezedried.Allwatermoleculeshavebeenremoved.
AntigenIncl.ThisantibodyisshippedwithitsantigenFREEofcharge!
- PeptideCHSEDEKLSFEAVR,correspondingtoaminoacidresidues56-69ofhumanSTIM1(Accession Q13586). STIM1expressedontheplasmamembrane:Extracellular,N-terminus.STIM1expressedontheER:Luminal,N-terminus.

Expression ofSTIM1inratpancreasImmunohistochemicalstainingofratparaffin-embeddedpancreassectionsusingAnti-STIM1(extracellular)-ATTO-550 Antibody(#ACC-063-AO),(1:20),(red).A.STIM1stainingishighlyspecificforendocrinecellsintheIsleofLangerhans.B.Hoechst33342(blue)isusedasthecounterstain.C.MergedimageofpanelsAandB.
ExpressionofSTIM1inRBLcellsCellsurfacedetectionofSTIM1inliveRBLcells.A.ExtracellularstainingofcellswithAnti-STIM1(extracellular)-ATTO-550 Antibody(#ACC-063-AO),(1:20).B.Nuclearstainingofcellsusingthecell-permeabledyeHoechst33342.C.MergedimageofpanelsAandB.
- 1.Eisner,D.A.etal.(2005)Exp.Physiol.90,3.
- 2.Chakrabarti,R.andChakrabarti,R.(2006)J.Cell.Biochem.99,1503.
- 3.Feske,S.etal.(2006)Nature441,179.
- 4.Pickett,J.(2006)NatureReviewsMol.CellBiol.7,794.
- 5.Dziadek,M.A.andJohnstone,L.S.(2007)CellCalcium42,123.
- 6.Yuan,J.P.etal.(2007)Nat.CellBiol.9,636.
- 7.Muik,M.etal.(2008)J.Biol.Chem.283,8014.
- 8.Li,Z.etal(2007)J.Biol.Chem.282,29448.
- 9.Hewavitharana,T.etal.(2007)CellCalcium42,173.
CytosolicCa2+ haslongbeenknowntoactasakeysecondmessengerinmanyintracellularpathwaysincludingsynaptictransmission,musclecontraction,hormonalsecretion,andcellgrowthandproliferation.1,2 ThemechanismcontrollingtheinfluxofintracellularCa2+ eitherbycalcium-release-activatedCa2+ channels(CRAC)orfromintracellularstoreshaslatelybecomeofgreatinterest.
Recently,severalkeyplayersofthestore-operatedcomplexhavebeenidentified.3 TheOraifamilyconsistsofthreemembers,Orai1-3,andtheSTIMfamily,whichconsistsoftwomembers,STIM1andSTIM2.Orai1(alsoknownasCRACM1)actsasthestore-operatedcalciumchannel(SOC)andSTIM1astheendoplasmicreticulum(ER)Ca2+ sensor.3,4 ThemajorityofSTIM1appearstobelocalizedintracellularlyattheERmembranewhilelowexpressionofSTIM1hasbeendetectedonthecellsurfaceofseveralcelltypes.5 STIM1hasanamino-terminalEFhandCa2+ bindingdomainfacingthelumenoftheER.6 UponCa2+ storedepletion,STIM1moleculesareredistributedinpunctaeunderneaththeplasmamembraneandactivateSOCs.
SeveralpossIBLeinteractionsbetweenSTIM1andOrai1havebeensuggested.ThemostsimpleandcitedisadynamicinteractionbetweenthecytosolicC-terminusofSTIM1andthecytoplasmicdomainoftheOrai1channel.7-9 STIM1isassumedtoregulatetheactivityofallknownSOCs,includingnativeSOCs.5 ConsistentwiththeirimportantroleascalciumsensorswithintheER,STIM1proteinsareubiquitouslyexpressed.
Anti-STIM1(extracellular)Antibody(#ACC-063)isahighlyspecificantibodydirectedagainstanextracellularepitopeofthehumanStromalinteractionmolecule1.Theantibodycanbeusedinwesternblot,immunocytochemistry,immunohistochemistry,andindirectflowcytometryapplications.IthasbeendesignedtorecognizeSTIM1fromhuman,rat,andmousesamples.
Anti-STIM1(extracellular)-ATTO-550Antibody(#ACC-063-AO)isdirectlylabeledwithanATTO-550fluorescentdye.ATTOdyesarecharacterizedbystrongabsorption(highextinctioncoefficient),highfluorescencequantumyield,andhighphoto-stABIlity.TheATTO-550fluorescentlabelisrelatedtothewellknowndyeRhodamine6GandcanbeusedwithfilterstypicallyusedtodetectRhodamine.Anti-STIM1(extracellular)-ATTO-550AntibodyhasbeentestedinimmunocytochemistryandimmunohistochemistryapplicationsandisespeciallysuitedforexperimentsrequiringsimultaneouslabelingofdifferentMarkers.
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比如你用anti-MHC-II,mouse IgG1-FITC,那同型就用非特异性的mouse IgG1-FITC,同样浓度
请问:同型对照应该怎么设?
不买专门的IgG2Bisotypecontrol,而用其他的抗体,
比如mouseanti-humanCD117,Igclass也是IgG2B,可以吗?
阴性对照(Isotype Control):非特异荧光的强弱取决于抗体浓度、单克隆荧光抗体特异性和纯度,应与试验管抗体相对应。在多色分析时,同型对照应与其它抗体同时使用,以避免补偿造成的误差
血小板体外活化试验:使用正常人活化标本作为阳性质控;使用正常人未活化标本作为阴性质控
血小板自身抗体检测:使用含有已知血小板抗体的血清与血小板孵育,作为阳性质控;使用不含血小板抗体的血清与血小板孵育,作为阴性质控
血小板表面抗原缺失:如巨血小板症血小板表面CD42a/CD42b缺失,血小板无力症血小板表面gpIIb/IIIa,即CD41/CD61缺失或异常。使用正常人标本做阳性对照,抗体的同型对照做阴性对照
英 ['aɪsətaɪp]美 ['aɪsəˌtaɪp]
n. 同型动物(或植物),图形文字,象征性图像;同位型;同模;同号模式
英 ['aisəutaip]
美 ['aisəutaip]
n. 同形像;
同形像统计图

