
About InVivoMAb anti-mouse B220
The RA3.3A1/6.1 monoclonal antibody reacts with mouse B220 also known as CD45R. B220 is a 220 kDa transmembrane protein tyrosine phosphatase expressed on B cells and some subsets of T and NK cells. B220 plays a critical role in TCR and BCR signaling and is commonly used as a B cell marker. The RA3.3A1/6.1 antibody is commonly used for in vivo B cell depletion.
InVivoMAb anti-mouse B220 Specifications
Isotype | Rat IgM |
Recommended Isotype Control(s) | InVivoMAb polyclonal rat IgG(BE0094) |
Recommended InVivoPure Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer(IP0070) |
Immunogen | Mouse RAW 112 lymphosarcoma cells |
Reported Applications |
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Endotoxin |
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Purity |
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Formulation |
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Sterility | 0.2 μM filtered |
Production | Purified from tissue culture supernatant in an animal free facility |
Purification | Protein A |
Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
RRID | AB_1107651 |
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英 ['aɪsətaɪp]美 ['aɪsəˌtaɪp]
n.
同型动物(或植物),图形文字,象征性图像;同位型;同模;同号模式
网络
同型;同种型;模式标本
双语例句
Study on the Action of Decreasing level of Isotype Cysteine for High Blood Viscosity
降低同型半胱氨酸水平对高黏血症作用的研究
比如你用anti-MHC-II,mouse IgG1-FITC,那同型就用非特异性的mouse IgG1-FITC,同样浓度
选用和特异性抗体同种亚型的,同样荧光素标记的抗体,采用同样的染色步骤染同样浓度的细胞。然后上机检测。如果细胞有非特异性的结合,那同型对照的荧光强度可能会比未染色的阴性对照要高一些。
同型对照只用于那种阳性和阴性之间没有明确分解的情况。如果阳性细胞群非常清楚,是不需要同型对照的。另外,在多色同染的情况下,也不采用同型对照,而是采用FMO(fluorensence minus one)对照。
另外,对于同型对照的使用,也有很多反对意见。就算是同型抗体,也可能会有不同的亲和力,并不一定就能真实反应非特异性的结合。

