About InVivoMAb anti-mouse/human/rat v-H-Ras
The Y13-238 monoclonal antibody reacts with human, mouse, and rat v-H-ras (within amino acids 120-138) and does not react with v-K-ras. v-H-ras binds GTP/GDP and has intrinsic GTPase activity. Ras proteins alternate between an inactive form bound to GDP and an active form bound to GTP, activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP). Under normal conditions, Ras family members influence cell growth and differentiation events. Mutations in the Ras family of proto-oncogenes are very common, being found in 20% to 30% of all human tumors. Ras point mutations are the single most common abnormality of human proto-oncogenes. The Y13-238 antibody does not neutralize ras GTPase binding and hydrolysis in vivo or in vitro.
InVivoMAb anti-mouse/human/rat v-H-Ras Specifications
| Isotype | Rat IgG2a |
| Recommended Isotype Control(s) | InVivoMAb rat IgG2a isotype control, anti-trinitrophenol(BE0089) |
| Recommended InVivoPure Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer(IP0070) |
| Immunogen | Harvey murine sarcoma virus infected NRK cells |
| Reported Applications | Immunoprecipitation |
| Endotoxin |
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| Purity |
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| Formulation |
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| Sterility | 0.2 μM filtered |
| Production | Purified from tissue culture supernatant in an animal free facility |
| Purification | Protein G |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| RRID | AB_10951512 |
| Molecular Weight | 150 kDa |
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英 ['aɪsətaɪp]美 ['aɪsəˌtaɪp]
n. 同型动物(或植物),图形文字,象征性图像;同位型;同模;同号模式
手头上,有eBioscience家的同型抗体,为PE标记(mouseIgG1,k),说明书写使用为2.5ug/test。
按照说明书的浓度加入后结果发现,同型对照抗体组比实验组和阴性对照组(不表达CD80)荧光强度要高,很明确我的同型对照加入的剂量多了,但正确的加法是多少?按照质量算吗?请高手指教,非常感谢!!
其同型为;percp-cy5.5(25ug包装)浓度:0.2mg/ml,
那么如果CD45RA抗体如果加入20ul,那同型抗体应该加入多少ml:
我自己的初步计算是:1ul的3分之1.不知道我算对了吗?应该怎么加才好呢?

