About InVivoMAb anti-mouse CD48
The HM48-1 monoclonal antibody reacts with mouse CD48 also known as BCM1, Blast-1 (human), and OX-45 (rat). CD48 is a 45 kDa GPI-anchored glycoprotein and a member of the SLAM family and Ig superfamily. CD48 is expressed on T and B lymphocytes as well as monocytes and macrophages. CD48 plays a critical role in adhesion and T cell activation. The primary ligands for CD48 are CD2 and CD244. The HM48-1 antibody is reported to block the CD48/CD2 and CD48/CD244 interactions in vivo as well as inhibit the proliferative response of mitogen-activated spleen cells, provide a co-stimulatory signal for activated T cells in vitro, and prolong cardiac allograft survival in vivo.
InVivoMAb anti-mouse CD48 Specifications
| Isotype | Armenian Hamster IgG |
| Recommended Isotype Control(s) | InVivoMAbpolyclonal Armenian hamster IgG(BE0091) |
| Recommended InVivoPure Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer(IP0070) |
| Immunogen | MBL2 mouse T lymphoma cells |
| Reported Applications |
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| Endotoxin |
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| Purity |
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| Formulation |
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| Sterility | 0.2 μM filtered |
| Production | Purified from tissue culture supernatant in an animal free facility |
| Purification | Protein G |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| RRID | AB_10949470 |
| Molecular Weight | 150 kDa |
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英 ['aɪsətaɪp]美 ['aɪsəˌtaɪp]
n. 同型动物(或植物),图形文字,象征性图像;同位型;同模;同号模式
手头上,有eBioscience家的同型抗体,为PE标记(mouseIgG1,k),说明书写使用为2.5ug/test。
按照说明书的浓度加入后结果发现,同型对照抗体组比实验组和阴性对照组(不表达CD80)荧光强度要高,很明确我的同型对照加入的剂量多了,但正确的加法是多少?按照质量算吗?请高手指教,非常感谢!!
其同型为;percp-cy5.5(25ug包装)浓度:0.2mg/ml,
那么如果CD45RA抗体如果加入20ul,那同型抗体应该加入多少ml:
我自己的初步计算是:1ul的3分之1.不知道我算对了吗?应该怎么加才好呢?

