
Product Description | Mouse monoclonal antibody to IE 1/2 of Cytomegalovirus. This antibody originates from ascites fluids and is purified by protein G agarose affinity chromatography. |
Catalog No. | P1215 |
Unit Size | 500 µg |
Species/Class | Mouse IgG1(k) |
Clone | CH160 |
Protein Concentration | 1 mg/ml (A280) |
Buffer | Phosphate Buffered Saline (PBS) pH 7.4 |
Purity | Protein G agarose affinity chromatography |
Functional Activity | Reactive with immediate early protein 1 and 2 of Cytomegalovirus in immunofluorescence (IFA) and western blots assays at 10 µg/ml. |
Shipping & Storage | This product is supplied frozen on dry ice. Upon receipt, store at -20°C. Avoid multiple freeze-thaw cycles as product degradation may result. |
References | Dondero, D.V., and Pereira, L. (1990). Monoclonal antibody production. In Diagnostic Procedures for Viral, Rickettsial and Chlamydial Infections, R. Emmons, and N. Schmidt, eds. (Washington, D.C., American Public Health Association), pp. 101-124. J Virol. Nov 2011; 85(21): 11098–11110 |
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想做个蛋白的定位,可是没有好抗体,就想做个转基因小鼠,在基因后面加一个标签。但是之前没有相关经验,不知道哪一种tag更好呢,更适合在小鼠里表达呢?因为我蛋白比较小,加GFP那么大的话可能会影响表达。比较小的tag例如HA,Flag还有His等,不知道哪个更适合一些?求大神们给些经验。
但是His标签是目前最普及最方便的融合标签,所以His标签的抗体,不管是单抗还是多抗,鼠源还是兔源都是商品化程度非常高的的抗体。这样的话因为抗体原因失败的可能性就非常小了。
百度上的,,,,,搬运
义翘神州的标签抗体(myc)采用的免疫原是A synthetic Myc tag peptide conjugated to KLH 。参看下图的验证结果。
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此酶溶于水和50%饱和度以下的硫酸铵溶液。
求助:为什么用蛋白抗体仅出来一条带,而用Flag抗体则出来两条带?
谢谢大家啊
图一:蛋白抗体
图二Flag抗体
例如 GST (1-109):
•epitope corresponding to amino acids 1-109 mapping at the N-terminus of GST of Schistosoma japonicum origin
•recommended for detection of GST fusion proteins and glutathione-S-transferase (GST) of Schistosoma japonicum origin by WB, IP, IF and ELISA
还有Schistosoma japonicum origin 跟我载体pGEX4T-1表达的融合蛋白可用Western Blot吗?它们有什么关系?
十分感谢

