Description:
(cat#100400/100401)
flashBAC™istheeasiestandthequickestbaculovirusproteinexpressionsystemavailable.TheflashBAC™expressionvectorsystemisanewplatformtechnologyfortheproductionofrecombinantbaculoviruses,geneticallyoptimizedforproducing100%recombinantvirusinasinglestepthuseliminatingtheneedforplaquepurificationorotherscreeningsystems.Thegeneticoptimizationnotonlypreventparentalvirusfromreplicating,butalsosignificantlyincreasesproteinyield.TheflashBAC™expressionsystemhasbeenspecificallydevelopedforoptimalproteinproductionininsectcells.TheflashBAC™DNAisabaculovirusgenomethathasbeengeneticallyoptimisedtofunctionasarecombinantproteinexpressionvector.Thesystemoffershighlevelsofproteinexpression,iseasytouseandisalsobackcompatIBLewithalltransfervectorsbasedonhomologousrecombination.InthiswaytheflashBAC™systemhasbecometheexpressionofchoiceforthoseneedingoptimalproteinexpressionininsectcells,andincreasinglyinmammaliancellsviatheBacMamsystem.
ThereareaseriesofflashBAC™expressionvectors,eachvaryinginthenumberofgenesdeletedfromthebaculovirusgenometosuitexpressionofdifferentproteins. TheseSelectionBoxesenableyoutotryoutdifferentversionsofflashBAC™inoneconvenientkit:SelectionBoxescontaineitherasingle3reactionkitof flashBAC™,flashBACGOLDandflashBACULTRA(Box1)orasingle3reactionkitof flashBAC,flashBACGOLD,flashBACULTRAandflashBACPRIME(Box2).TheoriginalflashBACexpressionvectorisagoodchoiceforprojectswherethegenetobeexpressedislikelytobesimpleandexpressedinthecytoplasmornucleus.flashBACGOLDisenhancedbythedeletionofthechitinaseandcathepsinproteasegenes.Itprovidessuperiorlevelsofexpressionforanyproteinthatisdestinedforsecretionortobeinsertedinthemembrane,orforanyproteinthatmightbeparticularlyliabletodegradation.flashBACULTRAcontainsdeletionsofthechitinaseandcathepsingenes,asabove,butalsodeletionsofthep10,p74andp26genesandprovidesthebestchoiceforthemostdifficulttoexpressproteins;uitableforcytoplasmic,nucleus,secretedandmembraneproteins.flashBACPRIMEcontainsnogenedeletionsandhasprovenusefulforthereleaseandsubsequentpurificationofintracellularVLPsandproteincomplexes. Ifitisastarterkityouareafter,baculoCOMPLETEistheperfectchoice.Thiskitcontainsallthecomponentsneededtogetstartedwithrecombinantproteinexpressionininsectcells.
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植物：抗虫、抗病、抗除草剂植物培育、改良作物品质（富含赖氨酸的玉米）
动物：提高生长速度、生产药物蛋白、器官移植、乳腺生物反应器
微生物：疫苗
基因治疗等等
各位业内前辈，我们正在考虑引进符合GMP认证标准的CHO细胞系，用于表达可做疫苗生产的重组蛋白类。目前已有符合标准信息的是Thermofisher的CHO-S悬浮培养细胞资料，希望能再多了解一些和这株细胞类似的其他公司符合GMP标准的生产株细胞做个比较。谢谢指教！
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考研细胞的题答案
生物合成人胰岛素注射液，比如诺和灵R笔芯。是单一品种、不是混合的胰岛素。是不含鱼精蛋白的短效胰岛素。
