货号：	hz551Bo01
供应商：	上海沪震
数量：	100
英文名：	RecombinantFibroblastGrowthFactor2,Basic(FGF2)
保质期：	12个月
保存条件：	-20℃

碱性成纤维细胞生长因子(FGF2)重组蛋白 RecombinantFibroblastGrowthFactor2,Basic(FGF2)
FORINVITROANDRESEARCHUSEONLY,NOTFORUSEINCLINICALDIAGNOSTICPROCEDURES!
Organismspecies   Bostaurus;Bovine(Cattle)
ProductNo.   RPA551Bo01
Source   Prokaryoticexpression
Host   E.coli
Purity   >95%
UOM   50ug
PredictedMolecularMass   16.4kDa
Predictedisoelectricpoint   n/a
Applications   SDS-PAGE;WB;ELISA;IP.
EndotoxinLevel   <1.0EUper1µg(determinedbytheLALmethod)
SubcellularLocation   n/a
Residues   Phe26~Ser155(Accession#P03969)withN-terminalHis-Tag
碱性成纤维细胞生长因子(FGF2)重组蛋白Formulation   SuppliedaslyophilizedforminPBS,pH7.4,containing5%trehalose,0.01%sarcosyl.
SEQUENCES
ThetargetproteinisfusedwithN-terminalHis-Tag,itssequenceislistedbelow.
MGHHHHHHSGSEF-FKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYSSWYVALKRTGQYKLGPKTGPGQKAILFLPMSAKS
USAGE
ReconstituteinsterilePBS,pH7.2-pH7.4.
STORAGEANDSTABILITY
Storage:Avoidrepeatedfreeze/thawcycles.Storeat2-8oCforonemonth.Aliquotandstoreat-80oCfor12months.
StabilityTest:Thethermalstabilityisdescribedbythelossrateofthetargetprotein.Thelossratewasdeterminedbyacceleratedthermaldegradationtest,thatis,incubatetheproteinat37oCfor48h,andnoobviousdegradationandprecipitationwereobserved.(ReferringfromChinaBiologicalProductsStandard,whichwascalculatedbytheArrheniusequation.)Thelossofthisproteinislessthan5%withintheexpirationdateunderappropriatestoragecondition.
AbouttheMarker(complimentary)
EffectiveSizeRange:10kDato70kDa.
Proteinbands:10kDa,14kDa,18kDa,22kDa,26kDa,33kDa,44kDaand70kDa.
Doubleintensitybands:The26kDa,18kDa,10kDabandsareatdoubleintensitytomakelocationandsizeapproximationofproteinsofinterestquickandeasy.
碱性成纤维细胞生长因子(FGF2)重组蛋白Ready-to-use:Noneedtoheat,diluteoraddreducingagentsbeforeuse.