Description:
flashBACGOLD(cat#100200/100201/100202/100203) providessuperiorlevelsofexpressionforanyproteinthatisdestinedforsecretionortobeinsertedinthemembrane,orforanyproteinthatmightbeparticularlyliabletodegradation.flashBACGOLDisabaculovirusexpressionvectorthathasbeendesignedtoreduceproteolysis,maximiseproteinsecretionandimprovemembraneproteintargetingandisbasedonourpatentedflashBAC™system.ThedeletionofbothchiAandv-cathgenesfromflashBACGOLDhasimprovedtheefficacyofthesecretorypathwayandresultedinagreatlyenhancedyieldofrecombinantproteinsthataresecretedormembranetargeted(incomparisontorecombinantvirusesthatencodechiAandv-cath).
UsingtheflashBAC™systemiseasierandquickerthanotherbaculovirusexpressionsystemsbecausethereisnoneedtoseparaterecombinantvirusfromparentalvirusbyplaque-purificationoranyothermeans;onlyrecombinantvirusisproducedaftertheco-transfection. Becausetheproductionofrecombinantvirushasbeenreducedtoaonestepprocedureininsectcells,itamenabletohighthroughputandautomatedproductionsystems. However,itisalsoofbenefittothesmallresearchgroupjustrequiringoneorafewrecombinantbaculovirusespreparedinindividualdishesofcells.
Baculovirusgenomescontainseveralauxillarygenes,whicharenon-essentialforreplicationininsectcellculture.Oneoftheseischitinase(chiA),whichencodesanenzymewithexo-andendochitinaseactivity.Inaninfectedinsect,chitinasetogetherwiththecysteineproteasecathepsin,facilitateshostcuticlebreakdownandtissueliquefactionattheverylatestagesofinfection,soreleasingthevirustoinfectmorehosts.ConfocalandelectronmicroscopyobservationsofinsectcellsinfectedwithAcMNPVhaveshownthatchitinaseistargetedtotheendoplasmicreticulumwhereitisdenselypackedinaparacrystallinearray,severelycompromisingthefunctionandefficacyofthesecretorypathway.DeletionofchiAfromflashBAC™hasimprovedtheefficacyofthesecretorypathwayandresultedinagreatlyenhancedyieldofsecretedandmembranetargetedproteins. Deletionofv-cathreducesthechancesoftherecombinantproteinbeingdegraded.
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植物：抗虫、抗病、抗除草剂植物培育、改良作物品质（富含赖氨酸的玉米）
动物：提高生长速度、生产药物蛋白、器官移植、乳腺生物反应器
微生物：疫苗
基因治疗等等
各位业内前辈，我们正在考虑引进符合GMP认证标准的CHO细胞系，用于表达可做疫苗生产的重组蛋白类。目前已有符合标准信息的是Thermofisher的CHO-S悬浮培养细胞资料，希望能再多了解一些和这株细胞类似的其他公司符合GMP标准的生产株细胞做个比较。谢谢指教！
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考研细胞的题答案
生物合成人胰岛素注射液，比如诺和灵R笔芯。是单一品种、不是混合的胰岛素。是不含鱼精蛋白的短效胰岛素。
